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Registro Completo |
Biblioteca(s): |
Embrapa Trigo. |
Data corrente: |
30/09/2016 |
Data da última atualização: |
03/10/2016 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
TOMELERO, V. J.; SCHMIDT, F.; DE BONA, F. D. |
Afiliação: |
VALMOR JOSÉ TOMELERO; FABIANA SCHMIDT, EPAGRI; FABIANO DANIEL DE BONA, CNPT. |
Título: |
Nutrição e produtividade da soja em função da aplicação de calcário e gesso no sistema plantio direto com e sem revolvimento do solo. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: REUNIÃO SUL-BRASILEIRA DE CIÊNCIA DO SOLO, 11., 2016, Frederico Westphalen. Qualidade do solo & ambiente de produção: [anais]. Pelotas: Sociedade Brasileira de Ciência do Solo - Núcleo Regional Sul, 2016. |
Páginas: |
4 p. |
Idioma: |
Português |
Notas: |
Fertilidade do Solo e Nutrição de Plantas. |
Palavras-Chave: |
Folhas diagnósticas; Gessagem; Nutrição de Plantas; Sistemas de preparo do solo; Teores de nutrientes. |
Thesagro: |
Calagem; Fertilidade do Solo; Glycine Max; Nodulação. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148117/1/ID43778-2016RSBCStrab-4-7867-175.pdf
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Marc: |
LEADER 01002nam a2200253 a 4500 001 2053877 005 2016-10-03 008 2016 bl uuuu u00u1 u #d 100 1 $aTOMELERO, V. J. 245 $aNutrição e produtividade da soja em função da aplicação de calcário e gesso no sistema plantio direto com e sem revolvimento do solo.$h[electronic resource] 260 $aIn: REUNIÃO SUL-BRASILEIRA DE CIÊNCIA DO SOLO, 11., 2016, Frederico Westphalen. Qualidade do solo & ambiente de produção: [anais]. Pelotas: Sociedade Brasileira de Ciência do Solo - Núcleo Regional Sul$c2016 300 $a4 p. 500 $aFertilidade do Solo e Nutrição de Plantas. 650 $aCalagem 650 $aFertilidade do Solo 650 $aGlycine Max 650 $aNodulação 653 $aFolhas diagnósticas 653 $aGessagem 653 $aNutrição de Plantas 653 $aSistemas de preparo do solo 653 $aTeores de nutrientes 700 1 $aSCHMIDT, F. 700 1 $aDE BONA, F. D.
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Registro original: |
Embrapa Trigo (CNPT) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
14/01/2020 |
Data da última atualização: |
06/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
FREITAS, V. J. F.; CAMPELO, I. S.; SILVA, M. M. A. S.; CAVALCANTI, C. M.; TEIXEIRA, D. I. A.; CAMARGO, L. S. de A.; MELO L. M.; RÁDIS-BAPTISTA, G. |
Afiliação: |
Vicente J. F. Freitas; Iana S. Campelo; Mirelly M .A. S. Silva; Camila M. Cavalcanti; Dárcio I. A. Teixeira; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Luciana M. Melo; Gandhi Rádis-Baptista. |
Título: |
Disulphide-less crotamine is effective for formation of DNA-peptide complex but is unable to improve bovine embryo transfection. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Zygote, v. 28, n. 1, p. 72-79, 2020. |
DOI: |
https://doi.org/10.1017/s0967199419000716 |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to investigate the ability of disulphide-less crotamine (dLCr) to complex DNA and to evaluate whether the DNA-dLCr complex is capable of improving transfection in bovine embryos. Three experiments were performed to: (i) evaluate the formation and stability of the DNA-dLCr complex; (ii) assess the dLCr embryotoxicity by exposure of bovine embryos to dLCr; and (iii) assess the efficiency of bovine embryo transfection after microinjection of the DNA-dLCr complex or green fluorescent protein (GFP) plasmid alone (control). DNA complexation by dLCr after 30 min of incubation at 1:100 and 1:50 proportions presented higher efficiency (P < 0.05) than the two controls: native crotamine (NCr) 1:10 and lipofectamine. There was no difference between DNA-dLCr 1:25 and the controls. The DNA-dLCr complexation was evaluated at different proportions and times. In all, at least half of maximum complexation was achieved within the initial 30 min. No embryotoxicity of dLCr was verified after exposure of in vitro fertilized embryos to different concentrations of the peptide. The effectiveness of dLCr to improve exogenous gene expression was evaluated by microinjection of the DNA-dLCr complex into in vitro fertilized zygotes, followed by verification of both embryo development and GFP expression. From embryos microinjected with DNA only, 4.6% and 2.8% expressed the GFP transgene at day 5 and day 7, respectively. The DNA-dLCr complex did not increase the number of GFP-positive embryos. In conclusion, dLCr forms a complex with DNA and its application in in vitro culture is possible. However, the dLCr peptide sequence should be redesigned to improve GFP expression. MenosThis study aimed to investigate the ability of disulphide-less crotamine (dLCr) to complex DNA and to evaluate whether the DNA-dLCr complex is capable of improving transfection in bovine embryos. Three experiments were performed to: (i) evaluate the formation and stability of the DNA-dLCr complex; (ii) assess the dLCr embryotoxicity by exposure of bovine embryos to dLCr; and (iii) assess the efficiency of bovine embryo transfection after microinjection of the DNA-dLCr complex or green fluorescent protein (GFP) plasmid alone (control). DNA complexation by dLCr after 30 min of incubation at 1:100 and 1:50 proportions presented higher efficiency (P < 0.05) than the two controls: native crotamine (NCr) 1:10 and lipofectamine. There was no difference between DNA-dLCr 1:25 and the controls. The DNA-dLCr complexation was evaluated at different proportions and times. In all, at least half of maximum complexation was achieved within the initial 30 min. No embryotoxicity of dLCr was verified after exposure of in vitro fertilized embryos to different concentrations of the peptide. The effectiveness of dLCr to improve exogenous gene expression was evaluated by microinjection of the DNA-dLCr complex into in vitro fertilized zygotes, followed by verification of both embryo development and GFP expression. From embryos microinjected with DNA only, 4.6% and 2.8% expressed the GFP transgene at day 5 and day 7, respectively. The DNA-dLCr complex did not increase the number of GFP-positive embr... Mostrar Tudo |
Palavras-Chave: |
Gene delivery; Microinjection; Nucleic acid-peptide complex. |
Thesaurus NAL: |
Embryotoxicity; Transgenesis. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02565naa a2200277 a 4500 001 2118715 005 2024-02-06 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1017/s0967199419000716$2DOI 100 1 $aFREITAS, V. J. F. 245 $aDisulphide-less crotamine is effective for formation of DNA-peptide complex but is unable to improve bovine embryo transfection.$h[electronic resource] 260 $c2020 520 $aThis study aimed to investigate the ability of disulphide-less crotamine (dLCr) to complex DNA and to evaluate whether the DNA-dLCr complex is capable of improving transfection in bovine embryos. Three experiments were performed to: (i) evaluate the formation and stability of the DNA-dLCr complex; (ii) assess the dLCr embryotoxicity by exposure of bovine embryos to dLCr; and (iii) assess the efficiency of bovine embryo transfection after microinjection of the DNA-dLCr complex or green fluorescent protein (GFP) plasmid alone (control). DNA complexation by dLCr after 30 min of incubation at 1:100 and 1:50 proportions presented higher efficiency (P < 0.05) than the two controls: native crotamine (NCr) 1:10 and lipofectamine. There was no difference between DNA-dLCr 1:25 and the controls. The DNA-dLCr complexation was evaluated at different proportions and times. In all, at least half of maximum complexation was achieved within the initial 30 min. No embryotoxicity of dLCr was verified after exposure of in vitro fertilized embryos to different concentrations of the peptide. The effectiveness of dLCr to improve exogenous gene expression was evaluated by microinjection of the DNA-dLCr complex into in vitro fertilized zygotes, followed by verification of both embryo development and GFP expression. From embryos microinjected with DNA only, 4.6% and 2.8% expressed the GFP transgene at day 5 and day 7, respectively. The DNA-dLCr complex did not increase the number of GFP-positive embryos. In conclusion, dLCr forms a complex with DNA and its application in in vitro culture is possible. However, the dLCr peptide sequence should be redesigned to improve GFP expression. 650 $aEmbryotoxicity 650 $aTransgenesis 653 $aGene delivery 653 $aMicroinjection 653 $aNucleic acid-peptide complex 700 1 $aCAMPELO, I. S. 700 1 $aSILVA, M. M. A. S. 700 1 $aCAVALCANTI, C. M. 700 1 $aTEIXEIRA, D. I. A. 700 1 $aCAMARGO, L. S. de A. 700 1 $aMELO L. M. 700 1 $aRÁDIS-BAPTISTA, G. 773 $tZygote$gv. 28, n. 1, p. 72-79, 2020.
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