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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
18/02/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARASHIRO, E. K. N.; PALHÃO, M. P.; WOHLRES-VIANA, S.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; HENRY, M. R. J. M.; VIANA, J. H. M. |
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Afiliação: |
EDUARDO KENJI N. ARASHIRO, UFMG; MILLER PEREIRA PALHÃO, UNIFENAS; SABINE WOHLRES-VIANA, UFJF; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARC ROGER JEAN MARIE HENRY, UFMG; JOAO HENRIQUE MOREIRA VIANA, CNPGL. |
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Título: |
In vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Reproductive Biology and Endocrinology, v. 11, article 73, 2013. |
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DOI: |
https://doi.org/10.1186/1477-7827-11-73 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Most studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters. Methods: In the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle. Results: In Trial I, the plasma recovery efficiency was similar (P > 0.05) for the volumes expected for 12 and 10 mm follicles, but decreased (P < 0.05) for smaller follicles (45.7+/-4.0%, 12.4+/-4.3% and 0.0+/-0.0% for 8, 6, and 4 mm follicles, respectively). Using the adaptation, the losses intrinsic to the aspiration system were similar for all follicle diameters. In Trial II, the expected and recovered volumes of FF were correlated (r = 0.89) and the efficiency of recovery was similar among follicles <12 mm, while larger follicles had a progressive increase in FF losses that was not related to the tubing system. In Trial III, the number of GC and amount of RNA obtained were not affected (P > 0.05) by follicle size, but differed according to breed (615,054+/-58,122 vs 458,095+/-36,407 for Holstein and Gir, respectively; P < 0.05). Conclusions: The adapted-OPU system can be successfully used for the in vivo collection of FF and GC from follicles of different diameters. This will enable further endocrine, cellular, and gene expression analyses. MenosBackground: Most studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters. Methods: In the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle. Results: In Trial I, the plasma recovery efficiency was similar (P > 0.05) for the v... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Follicle aspiration; Follicle biopsy. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03310naa a2200241 a 4500
001 1980418
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/1477-7827-11-73$2DOI
100 1 $aARASHIRO, E. K. N.
245 $aIn vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system.$h[electronic resource]
260 $c2013
520 $aBackground: Most studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters. Methods: In the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle. Results: In Trial I, the plasma recovery efficiency was similar (P > 0.05) for the volumes expected for 12 and 10 mm follicles, but decreased (P < 0.05) for smaller follicles (45.7+/-4.0%, 12.4+/-4.3% and 0.0+/-0.0% for 8, 6, and 4 mm follicles, respectively). Using the adaptation, the losses intrinsic to the aspiration system were similar for all follicle diameters. In Trial II, the expected and recovered volumes of FF were correlated (r = 0.89) and the efficiency of recovery was similar among follicles <12 mm, while larger follicles had a progressive increase in FF losses that was not related to the tubing system. In Trial III, the number of GC and amount of RNA obtained were not affected (P > 0.05) by follicle size, but differed according to breed (615,054+/-58,122 vs 458,095+/-36,407 for Holstein and Gir, respectively; P < 0.05). Conclusions: The adapted-OPU system can be successfully used for the in vivo collection of FF and GC from follicles of different diameters. This will enable further endocrine, cellular, and gene expression analyses.
653 $aBovine
653 $aFollicle aspiration
653 $aFollicle biopsy
700 1 $aPALHÃO, M. P.
700 1 $aWOHLRES-VIANA, S.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aCAMARGO, L. S. de A.
700 1 $aHENRY, M. R. J. M.
700 1 $aVIANA, J. H. M.
773 $tReproductive Biology and Endocrinology$gv. 11, article 73, 2013.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
02/03/2011 |
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Data da última atualização: |
02/03/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
TEIXEIRA, A. H. de C. |
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Afiliação: |
ANTONIO HERIBERTO DE C TEIXEIRA, CPATSA. |
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Título: |
Determinação do requerimento hídrico de videiras em função de métodos agrometeorológicos no Semiárido brasileiro. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: SBF, 2010. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
O objetivo deste trabalho é a utilização de parâmetros agrometeorológicos para a obtenção do requerimento de água de videiras para mesa e para vinho cultivadas sob diferentes sistemas de condução e de irrigação, visando subsidiar o manejo de água destas culturas nas condições semiáridas do Brasil. |
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Palavras-Chave: |
Consumo hídrico. |
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Thesagro: |
Balanço de Energia; Irrigação; Uva. |
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Thesaurus NAL: |
Energy balance; Grapes; Irrigation. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/28928/1/heriberto.pdf
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Marc: |
LEADER 01000nam a2200205 a 4500
001 1879711
005 2023-03-02
008 2010 bl uuuu u00u1 u #d
100 1 $aTEIXEIRA, A. H. de C.
245 $aDeterminação do requerimento hídrico de videiras em função de métodos agrometeorológicos no Semiárido brasileiro.
260 $aIn: CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: SBF$c2010
300 $c1 CD-ROM.
520 $aO objetivo deste trabalho é a utilização de parâmetros agrometeorológicos para a obtenção do requerimento de água de videiras para mesa e para vinho cultivadas sob diferentes sistemas de condução e de irrigação, visando subsidiar o manejo de água destas culturas nas condições semiáridas do Brasil.
650 $aEnergy balance
650 $aGrapes
650 $aIrrigation
650 $aBalanço de Energia
650 $aIrrigação
650 $aUva
653 $aConsumo hídrico
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