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Registro Completo |
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
22/03/2016 |
Data da última atualização: |
22/03/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, C. R. C.; PONTES, R. G. M. S. de; LIMA, L. M. de; MARTINS, E. S.; MELO FILHO, P. A.; PINHEIRO, M. P. N.; SANTOS, R. C. dos. |
Afiliação: |
CARLIANE R. C. SILVA, UFRPE; ROSE GOMES MONNERAT SOLON DE PONTES, CENARGEN; LIZIANE MARIA DE LIMA, CNPA; ÉRICA S. MARTINS, CENARGEN; PÉRICLES A. MELO FIILHO, UFRPE; MORGANNA P. N. PINHEIRO, UFRPE; ROSEANE CAVALCANTI DOS SANTOS, CNPA. |
Título: |
Stable integration and expression of a cry1 la gene conferring resistance to fall armyworm and boll weevil in cotton plants. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Pest Management Science, 2015. |
Idioma: |
Inglês |
Conteúdo: |
BACKGROUND: Boll weevil is a serious pest of cotton crop. Effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by usingmolecular tools. RESULTS: Four Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines. Complete gene integration was found in only one line (T0-34) containing one copy of cry1Ia detected by Southern blot. Protein was expressed in high concentration at 45 days after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A binding of cry1Ia antibody was found in the midgut of boll weevils fed on T0-34 buds in an immunodetection assay. CONCLUSION: The gene introduced into plants confers resistance to boll weevil and fall armyworm. Transmission of the transgene occurred normally to T1 progeny.All plants showed phenotypically normal growth,with fertile flowers and abundant seeds. |
Palavras-Chave: |
Chemical inseticides. |
Thesagro: |
Anthonomus grandis; Gossypium hirsutum; Spodoptera frugiperda. |
Thesaurus Nal: |
cotton. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141527/1/Stable-integration-and-expression-of-a-cry1-...pdf
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Marc: |
LEADER 02151naa a2200253 a 4500 001 2041613 005 2016-03-22 008 2015 bl uuuu u00u1 u #d 100 1 $aSILVA, C. R. C. 245 $aStable integration and expression of a cry1 la gene conferring resistance to fall armyworm and boll weevil in cotton plants.$h[electronic resource] 260 $c2015 520 $aBACKGROUND: Boll weevil is a serious pest of cotton crop. Effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by usingmolecular tools. RESULTS: Four Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines. Complete gene integration was found in only one line (T0-34) containing one copy of cry1Ia detected by Southern blot. Protein was expressed in high concentration at 45 days after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A binding of cry1Ia antibody was found in the midgut of boll weevils fed on T0-34 buds in an immunodetection assay. CONCLUSION: The gene introduced into plants confers resistance to boll weevil and fall armyworm. Transmission of the transgene occurred normally to T1 progeny.All plants showed phenotypically normal growth,with fertile flowers and abundant seeds. 650 $acotton 650 $aAnthonomus grandis 650 $aGossypium hirsutum 650 $aSpodoptera frugiperda 653 $aChemical inseticides 700 1 $aPONTES, R. G. M. S. de 700 1 $aLIMA, L. M. de 700 1 $aMARTINS, E. S. 700 1 $aMELO FILHO, P. A. 700 1 $aPINHEIRO, M. P. N. 700 1 $aSANTOS, R. C. dos 773 $tPest Management Science, 2015.
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Registro original: |
Embrapa Algodão (CNPA) |
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Registro Completo
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
23/05/2001 |
Data da última atualização: |
02/09/2021 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
TAVARES, S. C. C. de H.; AMORIM, L. R.; LIMA, J. A. S. |
Afiliação: |
SELMA C. CRUZ DE HOLANDA TAVARES, CPATSA. |
Título: |
Doenças do aspargo em área irrigada do trópico semi-árido brasileiro. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
Horticultura Brasileira, Brasilia, DF, v. 15, 1997. |
Idioma: |
Português |
Notas: |
Suplemento. Edição dos Resumos do 37. Congresso Brasileiro de Olericultura, Manaus, 1997. Resumo 313. |
Conteúdo: |
Apesar de ser uma cultura rústica tem-se observado morte de algumas plantas que motivaram a investigação da causa. |
Palavras-Chave: |
Cultivo; Diseases; Regiao semi-arida. |
Thesagro: |
Asparagus Officinalis; Aspargo; Doença; Fusarium Oxysporum; Rhizoctonia Solani. |
Categoria do assunto: |
A Sistemas de Cultivo |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/131814/1/8827-1.pdf
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Marc: |
LEADER 00873nam a2200241 a 4500 001 1134174 005 2021-09-02 008 1997 bl uuuu u00u1 u #d 100 1 $aTAVARES, S. C. C. de H. 245 $aDoenças do aspargo em área irrigada do trópico semi-árido brasileiro. 260 $aHorticultura Brasileira, Brasilia, DF, v. 15$c1997 500 $aSuplemento. Edição dos Resumos do 37. Congresso Brasileiro de Olericultura, Manaus, 1997. Resumo 313. 520 $aApesar de ser uma cultura rústica tem-se observado morte de algumas plantas que motivaram a investigação da causa. 650 $aAsparagus Officinalis 650 $aAspargo 650 $aDoença 650 $aFusarium Oxysporum 650 $aRhizoctonia Solani 653 $aCultivo 653 $aDiseases 653 $aRegiao semi-arida 700 1 $aAMORIM, L. R. 700 1 $aLIMA, J. A. S.
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