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Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
17/03/2021 |
Data da última atualização: |
17/03/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MOTA, T. R.; SOUZA, W. R. DE; OLIVEIRA, D. M.; MARTINS, P. K.; SAMPAIO, B. L.; VINECKY, F.; RIBEIRO, ANA. P.; DUARTE, K. E.; PACHECO, T. F.; MONTEIRO, N. DE K. V.; CAMPANHA, R. B.; MARCHIOSI, R.; VIEIRA, D. S.; KOBAYASHI, A. K.; OLIVEIRA, P. A. de; FERRARESE-FILHO, O.; MITCHELL, R. A. C.; MOLINARI, H. B. C.; SANTOS, W. D. D. |
Afiliação: |
THATIANE R. MOTA, Universidade Estadual de Maringá; WAGNER R. DE SOUZA; DYONI M. OLIVEIRA, Universidade Estadual de Maringá; POLYANA K. MARTINS; BRUNO L. SAMPAIO; FELIPE VINECKY; ANA P. RIBEIRO; KAROLINE E. DUARTE; THALYTA FRAGA PACHECO, CNPAE; NORBERTO DE K. V. MONTEIRO, Universidade Federal do Rio Grande do Norte; RAQUEL BOMBARDA CAMPANHA, CNPAE; ROGÉRIO MARCHIOSI, Universidade Estadual de Maringá; DAVI S. VIEIRA, Universidade Federal do Ceará; ADILSON KENJI KOBAYASHI, CNPAE; PATRICIA ABRAO DE OLIVEIRA MOLINARI, CNPAE; OSVALDO FERRARESE-FILHO, Universidade Estadual de Maringá; ROWAN A. C. MITCHELL, Rothamsted Research; HUGO BRUNO CORREA MOLINARI, CNPAE; WANDERLEY D. DOS SANTOS, Universidade Estadual de Maringá. |
Título: |
Suppression of a BAHD acyltransferase decreases p-coumaroyl on arabinoxylan and improves biomass digestibility in the model grass Setaria viridis. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
The Plant Journal, n. 105, p. 136-150, 2021. |
DOI: |
https://doi.org/10.1111/tpj.15046 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Grass cell walls have hydroxycinnamic acids attached to arabinosyl residues of arabinoxylan (AX), and certain BAHD acyltransferases are involved in their addition. In this study, we characterized one of these BAHD genes in the cell wall of the model grass Setaria viridis. RNAi silenced lines of S. viridis (SvBAHD05) presented a decrease of up to 42% of ester-linked p-coumarate (pCA) and 50% of pCA-arabinofuranosyl, across three generations. Biomass from SvBAHD05 silenced plants exhibited up to 32% increase in biomass saccharification after acid pre-treatment, with no change in total lignin. Molecular dynamics simulations suggested that SvBAHD05 is a p-coumaroyl coenzyme A transferase (PAT) mainly involved in the addition of pCA to the arabinofuranosyl residues of AX in Setaria. Thus, our results provide evidence of p-coumaroylation of AX promoted by SvBAHD05 acyltransferase in the cell wall of the model grass S. viridis. Furthermore, SvBAHD05 is a promising biotechnological target to engineer crops for improved biomass digestibility for biofuels, biorefineries and animal feeding. |
Palavras-Chave: |
Cell wall acylation; Grass xylan; Hydroxycinnamic acids. |
Thesagro: |
Lignina. |
Thesaurus Nal: |
Lignin; Lignocellulose; P-coumaric acid; Saccharification. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02426naa a2200445 a 4500 001 2130767 005 2021-03-17 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1111/tpj.15046$2DOI 100 1 $aMOTA, T. R. 245 $aSuppression of a BAHD acyltransferase decreases p-coumaroyl on arabinoxylan and improves biomass digestibility in the model grass Setaria viridis.$h[electronic resource] 260 $c2021 520 $aAbstract: Grass cell walls have hydroxycinnamic acids attached to arabinosyl residues of arabinoxylan (AX), and certain BAHD acyltransferases are involved in their addition. In this study, we characterized one of these BAHD genes in the cell wall of the model grass Setaria viridis. RNAi silenced lines of S. viridis (SvBAHD05) presented a decrease of up to 42% of ester-linked p-coumarate (pCA) and 50% of pCA-arabinofuranosyl, across three generations. Biomass from SvBAHD05 silenced plants exhibited up to 32% increase in biomass saccharification after acid pre-treatment, with no change in total lignin. Molecular dynamics simulations suggested that SvBAHD05 is a p-coumaroyl coenzyme A transferase (PAT) mainly involved in the addition of pCA to the arabinofuranosyl residues of AX in Setaria. Thus, our results provide evidence of p-coumaroylation of AX promoted by SvBAHD05 acyltransferase in the cell wall of the model grass S. viridis. Furthermore, SvBAHD05 is a promising biotechnological target to engineer crops for improved biomass digestibility for biofuels, biorefineries and animal feeding. 650 $aLignin 650 $aLignocellulose 650 $aP-coumaric acid 650 $aSaccharification 650 $aLignina 653 $aCell wall acylation 653 $aGrass xylan 653 $aHydroxycinnamic acids 700 1 $aSOUZA, W. R. DE 700 1 $aOLIVEIRA, D. M. 700 1 $aMARTINS, P. K. 700 1 $aSAMPAIO, B. L. 700 1 $aVINECKY, F. 700 1 $aRIBEIRO, ANA. P. 700 1 $aDUARTE, K. E. 700 1 $aPACHECO, T. F. 700 1 $aMONTEIRO, N. DE K. V. 700 1 $aCAMPANHA, R. B. 700 1 $aMARCHIOSI, R. 700 1 $aVIEIRA, D. S. 700 1 $aKOBAYASHI, A. K. 700 1 $aOLIVEIRA, P. A. de 700 1 $aFERRARESE-FILHO, O. 700 1 $aMITCHELL, R. A. C. 700 1 $aMOLINARI, H. B. C. 700 1 $aSANTOS, W. D. D. 773 $tThe Plant Journal$gn. 105, p. 136-150, 2021.
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Embrapa Agroenergia (CNPAE) |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
21/06/2021 |
Data da última atualização: |
21/06/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
VINCIGUERRA, L. L.; BÖCK, F. C.; SCHNEIDER, M. P.; REIS, N. A. P. C.; SILVA, L. F. da; SOUZA, K. C. M de; GUERRA, C. C.; GOMES, A. de A.; BERGOLD, A. M.; FERRÂO, M. F. |
Afiliação: |
LAYANE LENARDON VINCIGUERRA, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; FERNANDA CARLA BÖCK, Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; MATEUS PIRES SCHNEIDER, Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; NATALIA ALEJANDRA PISONI CANEDO REIS, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; LETICIA FLORES DA SILVA, CNPUV; KELLY CHRISTINA MENDES DE SOUZA, Instituto de Ciˆencias Exatas-ICE. Universidade Federal do Sul e Sudeste do Para-Unifesspa; CELITO CRIVELLARO GUERRA, CNPUV; ADRIANO DE ARAÚJO GOMES, Instituto de Ciˆencias Exatas-ICE. Universidade Federal do Sul e Sudeste do Pará-Unifesspa; ANA MARIA BERGOLD, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; MARCO FLÔRES FERRÂO, Instituto Nacional de Ciˆencia e Tecnologia-Bioanalítca (INCT-Bioanalítica), Cidade Universit´aria, Zeferino Vaz s/n, Campinas, São Paulo, Brazil. |
Título: |
Geographical origin authentication of southern Brazilian red wines by means of EEM-pH four-way data modelling coupled with one class classification approach. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Food Chemistry, v. 362, n. 130087, 2021. |
Idioma: |
Inglês |
Conteúdo: |
EEM data recorded at different pH values was exploited by MCR-ALS in order to determine qualitative information about Brazilian red wines. In addition, the geographical traceability of wines produced in the Serra Gaúcha (Rio Grande do Sul) was carried out by DD-SIMCA considering 53 samples from the target class and 20 from other producing regions. The fluorescence signal corresponds to 9 EEMs recorded at different pH (3?11), generating four-way data. By MCR-ALS decomposition, eight factors were retrieved and related to typical chemical compounds found in red wine. In addition, the EEM pH data was used to build a one-class classification model, considering that MCR scores and all samples of the target class were properly recognised as belonging to the target class, with maximal sensitivity equal to 1. Samples of the non-target class were also adequately rejected by the model, and the specificity was found to be 0.97. |
Palavras-Chave: |
MCR-ALS; Multiway data; One-class classification; Red wine; Serra Gaúcha. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/223909/1/Layane.Vinciguerra.et.al-Food.Chemistry-2021.pdf
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Marc: |
LEADER 01815naa a2200289 a 4500 001 2132416 005 2021-06-21 008 2021 bl uuuu u00u1 u #d 100 1 $aVINCIGUERRA, L. L. 245 $aGeographical origin authentication of southern Brazilian red wines by means of EEM-pH four-way data modelling coupled with one class classification approach.$h[electronic resource] 260 $c2021 520 $aEEM data recorded at different pH values was exploited by MCR-ALS in order to determine qualitative information about Brazilian red wines. In addition, the geographical traceability of wines produced in the Serra Gaúcha (Rio Grande do Sul) was carried out by DD-SIMCA considering 53 samples from the target class and 20 from other producing regions. The fluorescence signal corresponds to 9 EEMs recorded at different pH (3?11), generating four-way data. By MCR-ALS decomposition, eight factors were retrieved and related to typical chemical compounds found in red wine. In addition, the EEM pH data was used to build a one-class classification model, considering that MCR scores and all samples of the target class were properly recognised as belonging to the target class, with maximal sensitivity equal to 1. Samples of the non-target class were also adequately rejected by the model, and the specificity was found to be 0.97. 653 $aMCR-ALS 653 $aMultiway data 653 $aOne-class classification 653 $aRed wine 653 $aSerra Gaúcha 700 1 $aBÖCK, F. C. 700 1 $aSCHNEIDER, M. P. 700 1 $aREIS, N. A. P. C. 700 1 $aSILVA, L. F. da 700 1 $aSOUZA, K. C. M de 700 1 $aGUERRA, C. C. 700 1 $aGOMES, A. de A. 700 1 $aBERGOLD, A. M. 700 1 $aFERRÂO, M. F. 773 $tFood Chemistry$gv. 362, n. 130087, 2021.
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