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| Registros recuperados : 15 | |
| 1. |  | STEINDORFF, A. S.; SOUZA, M. S.; ALMEIDA, J. R. M. de; FORMIGHIERI, E. F. Genomic and mitochondrial assembly of xylose-fermenting yeasts from Debaryomycetaceae family. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa, 2016. p. 48-54.| Biblioteca(s): Embrapa Agroenergia. |
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| 4. | | PORTO, B. N.; STEINDORFF, A. S.; BRITO, L. S. de; FORMIGHIERI, E. F. Specific Quality Control is essential for Next-Generation Sequencing data usage: case studies in Illumina data from algae, yeasts and plants. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa, 2016. p. 77-85.| Biblioteca(s): Embrapa Agroenergia. |
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| 5. | | STEINDORFF, A. S.; FORMIGHIERI, E. F.; FARIA, F. P. de; POÇAS-FONSECA, M. J.; ALMEIDA, J. R. M. de. Genome sequencing of thermofilic fungus Humicola grisea var. thermoidea and effect of pH in sugarcane biomass transformation. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 4.,2017, Brasília, DF. Anais... Brasília, DF: Embrapa, 2017. p. 53-59.| Biblioteca(s): Embrapa Agroenergia. |
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| 6. | | PASCOAL, P. V.; HADI, S. I. I. A.; STEINDORFF, A. S.; FORMIGHIERI, E. F.; BRASIL, B. dos S. A. F. Sequenciamento do DNA mitocondrial de Chlamydomonas biconvexa revela um mitogenoma compacto. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 4.,2017, Brasília, DF. Anais... Brasília, DF: Embrapa, 2017. p. 46-52.| Biblioteca(s): Embrapa Agroenergia. |
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| 7. | | TRICHEZ, D.; STEINDORFF, A. S.; SOARES, C. E. V. F.; FORMIGHIERI, E. F.; ALMEIDA, J. R. M. de. Xylitol production: physiological and genetic characterization of new yeast strains. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 2017, 39., San Francisco, US. [Proceedings ...]. Fairfax: Society for Industrial Microbiology and Biotechnology, 2017. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 8. | | TRICHEZ, D.; CARNEIRO, C. V. G. C.; SOARES, C. E. V. F.; STEINDORFF, A. S.; FORMIGHIERI, E. F.; ALMEIDA, J. R. M. de. Biodiversity bioprospecting for xylitol production: selection, physiological and genetic characterization of new yeast strains. In: INTERNATIONAL SPECIALISED SYMPOSIUM ON YEAST - ISSY, 33., 2017, Cork, Ireland. [Proceedings ...]. [S.l]: International Commission on Yeasts, 2017. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 9. | | SANTIAGO, T. R.; DUARTE, K. E.; SOUZA, W. R. de; STEINDORFF, A. S.; FORMIGHIERI, E. F.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Divergence of DNA methylation and gene expression in Setaria viridis accessions under drought stress. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 31.| Biblioteca(s): Embrapa Agroenergia. |
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| 10. | | TRICHEZ, D.; STEINDORFF, A. S.; MORAIS JÚNIOR, W. G. de; VILELA, N.; BERGMANN, J. C.; FORMIGHIERI, E. F.; GONCALVES, S. B.; ALMEIDA, J. R. M. de. Identification of traits to improve co-assimilation of glucose and xylose by adaptive evolution of Spathaspora passalidarum and Scheffersomyces stipitis yeasts. Applied Microbiology and Biotechnology, n. 107, p. 1143?1157, Feb. 2023.| Biblioteca(s): Embrapa Agroenergia. |
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| 11. | | STEINDORFF, A. S.; SERRA, L. A.; FORMIGHIERI, E. F.; FARIA, F. P. de; POÇAS-FONSECA, M. J.; ALMEIDA, J. R. M. de. Insights into the Lignocellulose-Degrading Enzyme System of Humicola grisea var. thermoidea Based on Genome and Transcriptome Analysis. Microbiology Spectrum, v. 9, n. 2, e01088-21, 2021. PDF: il. color.| Biblioteca(s): Embrapa Agroenergia. |
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| 12. | | LOPES, F. A. C.; STEINDORFF, A. S.; GERALDINE, A. M.; BRANDÃO, R. S.; MONTEIRO, V. N.; LOBO JUNIOR, M.; COELHO, A. S. G.; ULHOA, C. J.; SILVA, R. N. Biochemical and metabolic profiles of Trichoderma strains isolated from common bean crops in the Brazilian Cerrado, and potential antagonism against Sclerotinia sclerotiorum. Fungal Biology, Oxford, v. 116, p. 815-824, 2012.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 13. | | BLANCO, A. J. V.; COSTA, M. O.; SILVA, R. do N.; ALBUQUERQUE, F. S. de; MELO, A. T. de O.; LOPES, F. A. C.; STEINDORFF, A. S.; BARBOSA, E. T.; ULHOA, C.; LOBO JUNIOR, M. Diversity and pathogenicity of rhizoctonia species from the brazilian cerrado. Plant Disease, v. 102, n. 4, p. 773-781, Apr. 2018.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 14. | | SANTIAGO, T. R.; PEREIRA, V. M.; SOUZA, W. R.; STEINDORFF, A. S.; DIAS, B. B. A.; GASPAR, M.; FAVARO, L. C. de L.; FORMIGHIERI, E. F.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Genome-wide identification, characterization and expression profile analysis of expansins gene family in sugarcane (Saccharum spp.). PLoS One, v. 13, n. 1, artigo e0191081, 2018.| Biblioteca(s): Embrapa Agroenergia. |
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| 15. | | LEITÃO, V. O.; NORONHA, E. T.; CAMARGO, B. R.; HAMANN, P. R. V.; STEINDORFF, A. S.; QUIRINO, B. F.; SOUSA, M. V. de; ULHOA, C. J.; FELIX, C. R. Growth and expression of relevant metabolic genes of Clostridium thermocellum cultured on lignocellulosic residues. Journal of Industrial Microbiology and Biotechnology, v. 44, n. 6, p. 825-834, 2017.| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 15 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
18/01/2023 |
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Data da última atualização: |
26/10/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
TRICHEZ, D.; STEINDORFF, A. S.; MORAIS JÚNIOR, W. G. de; VILELA, N.; BERGMANN, J. C.; FORMIGHIERI, E. F.; GONCALVES, S. B.; ALMEIDA, J. R. M. de. |
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Afiliação: |
DÉBORA TRICHEZ, CNPAE; ANDREI S. STEINDORFF, CNPAE/ US DOE JOINT GENOME INSTITUTE; WILSON G. DE MORAIS JÚNIOR, CNPAE/ MILHOUSE INTERNATIONAL PTY; NATHÁLIA VILELA, CNPAE; JESSICA CARVALHO BERGMANN; EDUARDO FERNANDES FORMIGHIERI, CNPAE; SILVIA BELEM GONCALVES, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
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Título: |
Identification of traits to improve co-assimilation of glucose and xylose by adaptive evolution of Spathaspora passalidarum and Scheffersomyces stipitis yeasts. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Applied Microbiology and Biotechnology, n. 107, p. 1143?1157, Feb. 2023. |
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DOI: |
https://doi.org/10.1007/s00253-023-12362-1 |
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Idioma: |
Inglês |
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Conteúdo: |
Lignocellulosic biomass is a renewable raw material for producing several high-value-added chemicals and fuels. In general, xylose and glucose are the major sugars in biomass hydrolysates, and their efficient utilization by microorganisms is critical for an economical production process. Yeasts capable of co-consuming mixed sugars might lead to higher yields and productivities in industrial fermentation processes. Herein, we performed adaptive evolution assays with two xylose-fermenting yeasts, Spathaspora passalidarum and Scheffersomyces stipitis, to obtain derived clones with improved capabilities of glucose and xylose co-consumption. Adapted strains were obtained after successive growth selection using xylose and the non-metabolized glucose analog 2-deoxy-D-glucose as a selective pressure. The co-fermentation capacity of evolved and parental strains was evaluated on xylose-glucose mixtures. Our results revealed an improved co-assimilation capability by the evolved strains; however, xylose and glucose consumption were observed at slower rates than the parental yeasts. Genome resequencing of the evolved strains revealed genes affected by non-synonymous variants that might be involved with the co-consumption phenotype, including the HXT2.4 gene that encodes a putative glucose transporter in Sp. passalidarum. Expression of this mutant HXT2.4 in Saccharomyces cerevisiae improved the cells? co-assimilation of glucose and xylose. Therefore, our results demonstrated the successful improvement of co-fermentation through evolutionary engineering and the identification of potential targets for further genetic engineering of different yeast strains. MenosLignocellulosic biomass is a renewable raw material for producing several high-value-added chemicals and fuels. In general, xylose and glucose are the major sugars in biomass hydrolysates, and their efficient utilization by microorganisms is critical for an economical production process. Yeasts capable of co-consuming mixed sugars might lead to higher yields and productivities in industrial fermentation processes. Herein, we performed adaptive evolution assays with two xylose-fermenting yeasts, Spathaspora passalidarum and Scheffersomyces stipitis, to obtain derived clones with improved capabilities of glucose and xylose co-consumption. Adapted strains were obtained after successive growth selection using xylose and the non-metabolized glucose analog 2-deoxy-D-glucose as a selective pressure. The co-fermentation capacity of evolved and parental strains was evaluated on xylose-glucose mixtures. Our results revealed an improved co-assimilation capability by the evolved strains; however, xylose and glucose consumption were observed at slower rates than the parental yeasts. Genome resequencing of the evolved strains revealed genes affected by non-synonymous variants that might be involved with the co-consumption phenotype, including the HXT2.4 gene that encodes a putative glucose transporter in Sp. passalidarum. Expression of this mutant HXT2.4 in Saccharomyces cerevisiae improved the cells? co-assimilation of glucose and xylose. Therefore, our results demonstrated the successfu... Mostrar Tudo |
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Thesaurus NAL: |
Fermentation; Glucose; Sugar products. |
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Categoria do assunto: |
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Marc: |
LEADER 02532naa a2200253 a 4500
001 2151042
005 2023-10-26
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00253-023-12362-1$2DOI
100 1 $aTRICHEZ, D.
245 $aIdentification of traits to improve co-assimilation of glucose and xylose by adaptive evolution of Spathaspora passalidarum and Scheffersomyces stipitis yeasts.$h[electronic resource]
260 $c2023
520 $aLignocellulosic biomass is a renewable raw material for producing several high-value-added chemicals and fuels. In general, xylose and glucose are the major sugars in biomass hydrolysates, and their efficient utilization by microorganisms is critical for an economical production process. Yeasts capable of co-consuming mixed sugars might lead to higher yields and productivities in industrial fermentation processes. Herein, we performed adaptive evolution assays with two xylose-fermenting yeasts, Spathaspora passalidarum and Scheffersomyces stipitis, to obtain derived clones with improved capabilities of glucose and xylose co-consumption. Adapted strains were obtained after successive growth selection using xylose and the non-metabolized glucose analog 2-deoxy-D-glucose as a selective pressure. The co-fermentation capacity of evolved and parental strains was evaluated on xylose-glucose mixtures. Our results revealed an improved co-assimilation capability by the evolved strains; however, xylose and glucose consumption were observed at slower rates than the parental yeasts. Genome resequencing of the evolved strains revealed genes affected by non-synonymous variants that might be involved with the co-consumption phenotype, including the HXT2.4 gene that encodes a putative glucose transporter in Sp. passalidarum. Expression of this mutant HXT2.4 in Saccharomyces cerevisiae improved the cells? co-assimilation of glucose and xylose. Therefore, our results demonstrated the successful improvement of co-fermentation through evolutionary engineering and the identification of potential targets for further genetic engineering of different yeast strains.
650 $aFermentation
650 $aGlucose
650 $aSugar products
700 1 $aSTEINDORFF, A. S.
700 1 $aMORAIS JÚNIOR, W. G. de
700 1 $aVILELA, N.
700 1 $aBERGMANN, J. C.
700 1 $aFORMIGHIERI, E. F.
700 1 $aGONCALVES, S. B.
700 1 $aALMEIDA, J. R. M. de
773 $tApplied Microbiology and Biotechnology$gn. 107, p. 1143?1157, Feb. 2023.
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