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| Registros recuperados : 15 | |
| 1. |  | STEINDORFF, A. S.; SOUZA, M. S.; ALMEIDA, J. R. M. de; FORMIGHIERI, E. F. Genomic and mitochondrial assembly of xylose-fermenting yeasts from Debaryomycetaceae family. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa, 2016. p. 48-54.| Biblioteca(s): Embrapa Agroenergia. |
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| 4. | | PORTO, B. N.; STEINDORFF, A. S.; BRITO, L. S. de; FORMIGHIERI, E. F. Specific Quality Control is essential for Next-Generation Sequencing data usage: case studies in Illumina data from algae, yeasts and plants. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa, 2016. p. 77-85.| Biblioteca(s): Embrapa Agroenergia. |
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| 5. | | STEINDORFF, A. S.; FORMIGHIERI, E. F.; FARIA, F. P. de; POÇAS-FONSECA, M. J.; ALMEIDA, J. R. M. de. Genome sequencing of thermofilic fungus Humicola grisea var. thermoidea and effect of pH in sugarcane biomass transformation. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 4.,2017, Brasília, DF. Anais... Brasília, DF: Embrapa, 2017. p. 53-59.| Biblioteca(s): Embrapa Agroenergia. |
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| 6. | | PASCOAL, P. V.; HADI, S. I. I. A.; STEINDORFF, A. S.; FORMIGHIERI, E. F.; BRASIL, B. dos S. A. F. Sequenciamento do DNA mitocondrial de Chlamydomonas biconvexa revela um mitogenoma compacto. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 4.,2017, Brasília, DF. Anais... Brasília, DF: Embrapa, 2017. p. 46-52.| Biblioteca(s): Embrapa Agroenergia. |
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| 7. | | TRICHEZ, D.; STEINDORFF, A. S.; SOARES, C. E. V. F.; FORMIGHIERI, E. F.; ALMEIDA, J. R. M. de. Xylitol production: physiological and genetic characterization of new yeast strains. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 2017, 39., San Francisco, US. [Proceedings ...]. Fairfax: Society for Industrial Microbiology and Biotechnology, 2017. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 8. | | TRICHEZ, D.; CARNEIRO, C. V. G. C.; SOARES, C. E. V. F.; STEINDORFF, A. S.; FORMIGHIERI, E. F.; ALMEIDA, J. R. M. de. Biodiversity bioprospecting for xylitol production: selection, physiological and genetic characterization of new yeast strains. In: INTERNATIONAL SPECIALISED SYMPOSIUM ON YEAST - ISSY, 33., 2017, Cork, Ireland. [Proceedings ...]. [S.l]: International Commission on Yeasts, 2017. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 9. | | TRICHEZ, D.; STEINDORFF, A. S.; MORAIS JÚNIOR, W. G. de; VILELA, N.; BERGMANN, J. C.; FORMIGHIERI, E. F.; GONCALVES, S. B.; ALMEIDA, J. R. M. de. Identification of traits to improve co-assimilation of glucose and xylose by adaptive evolution of Spathaspora passalidarum and Scheffersomyces stipitis yeasts. Applied Microbiology and Biotechnology, n. 107, p. 1143?1157, Feb. 2023.| Biblioteca(s): Embrapa Agroenergia. |
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| 10. | | SANTIAGO, T. R.; DUARTE, K. E.; SOUZA, W. R. de; STEINDORFF, A. S.; FORMIGHIERI, E. F.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Divergence of DNA methylation and gene expression in Setaria viridis accessions under drought stress. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 31.| Biblioteca(s): Embrapa Agroenergia. |
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| 11. | | STEINDORFF, A. S.; SERRA, L. A.; FORMIGHIERI, E. F.; FARIA, F. P. de; POÇAS-FONSECA, M. J.; ALMEIDA, J. R. M. de. Insights into the Lignocellulose-Degrading Enzyme System of Humicola grisea var. thermoidea Based on Genome and Transcriptome Analysis. Microbiology Spectrum, v. 9, n. 2, e01088-21, 2021. PDF: il. color.| Biblioteca(s): Embrapa Agroenergia. |
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| 12. | | LOPES, F. A. C.; STEINDORFF, A. S.; GERALDINE, A. M.; BRANDÃO, R. S.; MONTEIRO, V. N.; LOBO JUNIOR, M.; COELHO, A. S. G.; ULHOA, C. J.; SILVA, R. N. Biochemical and metabolic profiles of Trichoderma strains isolated from common bean crops in the Brazilian Cerrado, and potential antagonism against Sclerotinia sclerotiorum. Fungal Biology, Oxford, v. 116, p. 815-824, 2012.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 13. | | LEITÃO, V. O.; NORONHA, E. T.; CAMARGO, B. R.; HAMANN, P. R. V.; STEINDORFF, A. S.; QUIRINO, B. F.; SOUSA, M. V. de; ULHOA, C. J.; FELIX, C. R. Growth and expression of relevant metabolic genes of Clostridium thermocellum cultured on lignocellulosic residues. Journal of Industrial Microbiology and Biotechnology, v. 44, n. 6, p. 825-834, 2017.| Biblioteca(s): Embrapa Agroenergia. |
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| 14. | | BLANCO, A. J. V.; COSTA, M. O.; SILVA, R. do N.; ALBUQUERQUE, F. S. de; MELO, A. T. de O.; LOPES, F. A. C.; STEINDORFF, A. S.; BARBOSA, E. T.; ULHOA, C.; LOBO JUNIOR, M. Diversity and pathogenicity of rhizoctonia species from the brazilian cerrado. Plant Disease, v. 102, n. 4, p. 773-781, Apr. 2018.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 15. | | SANTIAGO, T. R.; PEREIRA, V. M.; SOUZA, W. R.; STEINDORFF, A. S.; DIAS, B. B. A.; GASPAR, M.; FAVARO, L. C. de L.; FORMIGHIERI, E. F.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Genome-wide identification, characterization and expression profile analysis of expansins gene family in sugarcane (Saccharum spp.). PLoS One, v. 13, n. 1, artigo e0191081, 2018.| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 15 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
15/09/2021 |
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Data da última atualização: |
15/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
STEINDORFF, A. S.; SERRA, L. A.; FORMIGHIERI, E. F.; FARIA, F. P. de; POÇAS-FONSECA, M. J.; ALMEIDA, J. R. M. de. |
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Afiliação: |
ANDREI STECCA STEINDORFF; LUANA ASSIS SERRA, Universidade de Brasília; EDUARDO FERNANDES FORMIGHIERI, CNPAE; FABRÍCIA PAULA DE FARIA, Universidade Federal de Goiás; MARCIO JOSÉ POÇAS-FONSECA, Universidade de Brasília; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
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Título: |
Insights into the Lignocellulose-Degrading Enzyme System of Humicola grisea var. thermoidea Based on Genome and Transcriptome Analysis. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Microbiology Spectrum, v. 9, n. 2, e01088-21, 2021. |
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Descrição Física: |
PDF: il. color. |
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DOI: |
https://doi.org/10.1128/Spectrum .01088-21 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Humicola grisea var. thermoidea is a thermophilic ascomycete and important enzyme producer that has an efficient enzymatic system with a broad spectrum of thermostable carbohydrate-active (CAZy) enzymes. These enzymes can be employed in lignocellulose biomass deconstruction and other industrial applications. In this work, the genome of H. grisea var. thermoidea was sequenced. The acquired sequence reads were assembled into a total length of 28.75 Mbp. Genome features correlate with what was expected for thermophilic Sordariomycetes. The transcriptomic data showed that sugar-cane bagasse significantly upregulated genes related to primary metabolism and polysaccharide deconstruction, especially hydrolases, at both pH 5 and pH 8. However, a number of exclusive and shared genes between the pH values were found, especially at pH 8. H. grisea expresses an average of 211 CAZy enzymes (CAZymes), which are capable of acting in different substrates. The top upregulated genes at both pH values represent CAZyme-encoding genes from different classes, including acetylxylan esterase, endo-1,4-b-mannosidase, exoglucanase, and endoglucanase genes. For the first time, the arsenal that the thermophilic fungus H. grisea var. thermoidea possesses to degrade the lignocellulosic biomass is shown. Carbon source and pH are of pivotal importance in regulating gene expression in this organism, and alkaline pH is a key regulatory factor for sugarcane bagasse hydrolysis. This work paves the way for the genetic manipulation and robust biotechnological applications of this fungus. MenosAbstract: Humicola grisea var. thermoidea is a thermophilic ascomycete and important enzyme producer that has an efficient enzymatic system with a broad spectrum of thermostable carbohydrate-active (CAZy) enzymes. These enzymes can be employed in lignocellulose biomass deconstruction and other industrial applications. In this work, the genome of H. grisea var. thermoidea was sequenced. The acquired sequence reads were assembled into a total length of 28.75 Mbp. Genome features correlate with what was expected for thermophilic Sordariomycetes. The transcriptomic data showed that sugar-cane bagasse significantly upregulated genes related to primary metabolism and polysaccharide deconstruction, especially hydrolases, at both pH 5 and pH 8. However, a number of exclusive and shared genes between the pH values were found, especially at pH 8. H. grisea expresses an average of 211 CAZy enzymes (CAZymes), which are capable of acting in different substrates. The top upregulated genes at both pH values represent CAZyme-encoding genes from different classes, including acetylxylan esterase, endo-1,4-b-mannosidase, exoglucanase, and endoglucanase genes. For the first time, the arsenal that the thermophilic fungus H. grisea var. thermoidea possesses to degrade the lignocellulosic biomass is shown. Carbon source and pH are of pivotal importance in regulating gene expression in this organism, and alkaline pH is a key regulatory factor for sugarcane bagasse hydrolysis. This work paves the wa... Mostrar Tudo |
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Palavras-Chave: |
CAZy enzymes; Genome sequencing; Humicola grisea; PH regulation. |
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Thesagro: |
Bagaço; Cana de Açúcar; Variação Genética. |
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Thesaurus NAL: |
Genome; Humicola; Sugarcane bagasse; Transcriptome. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226063/1/Insights-into-the-Lignocellulose-Degrading-Enzyme-System-2021.pdf
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Marc: |
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024 7 $ahttps://doi.org/10.1128/Spectrum .01088-21$2DOI
100 1 $aSTEINDORFF, A. S.
245 $aInsights into the Lignocellulose-Degrading Enzyme System of Humicola grisea var. thermoidea Based on Genome and Transcriptome Analysis.$h[electronic resource]
260 $c2021
300 $cPDF: il. color.
520 $aAbstract: Humicola grisea var. thermoidea is a thermophilic ascomycete and important enzyme producer that has an efficient enzymatic system with a broad spectrum of thermostable carbohydrate-active (CAZy) enzymes. These enzymes can be employed in lignocellulose biomass deconstruction and other industrial applications. In this work, the genome of H. grisea var. thermoidea was sequenced. The acquired sequence reads were assembled into a total length of 28.75 Mbp. Genome features correlate with what was expected for thermophilic Sordariomycetes. The transcriptomic data showed that sugar-cane bagasse significantly upregulated genes related to primary metabolism and polysaccharide deconstruction, especially hydrolases, at both pH 5 and pH 8. However, a number of exclusive and shared genes between the pH values were found, especially at pH 8. H. grisea expresses an average of 211 CAZy enzymes (CAZymes), which are capable of acting in different substrates. The top upregulated genes at both pH values represent CAZyme-encoding genes from different classes, including acetylxylan esterase, endo-1,4-b-mannosidase, exoglucanase, and endoglucanase genes. For the first time, the arsenal that the thermophilic fungus H. grisea var. thermoidea possesses to degrade the lignocellulosic biomass is shown. Carbon source and pH are of pivotal importance in regulating gene expression in this organism, and alkaline pH is a key regulatory factor for sugarcane bagasse hydrolysis. This work paves the way for the genetic manipulation and robust biotechnological applications of this fungus.
650 $aGenome
650 $aHumicola
650 $aSugarcane bagasse
650 $aTranscriptome
650 $aBagaço
650 $aCana de Açúcar
650 $aVariação Genética
653 $aCAZy enzymes
653 $aGenome sequencing
653 $aHumicola grisea
653 $aPH regulation
700 1 $aSERRA, L. A.
700 1 $aFORMIGHIERI, E. F.
700 1 $aFARIA, F. P. de
700 1 $aPOÇAS-FONSECA, M. J.
700 1 $aALMEIDA, J. R. M. de
773 $tMicrobiology Spectrum$gv. 9, n. 2, e01088-21, 2021.
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Embrapa Agroenergia (CNPAE) |
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