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| Registros recuperados : 142 | |
| 61. |  | FIGUEIRA, L. M.; ALVES, N. G.; SOUZA-FABJAN, J. M. G.; BATISTA, R. I. T. P.; SOUZA, L. C.; MAIA, A. L. R. S.; BRAIR, V. L.; FILGUEIRAS, M.; SOUZA, G. N. de; FONSECA, J. F. da. Superovulation and transcervical embryo recovery in Lacaune ewes raised under tropical conditions. Animal Reproduction, v. 15, n. 3, p. 402, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Biblioteca(s): Embrapa Gado de Leite. |
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| 62. | | FIGUEIRA, L. M.; ALVES, N. G.; SOUZA-FABJAN, J. M. G.; BATISTA, R. I. T. P.; SOUZA, L. C.; MAIA, A. L. R. S.; BRAIR, V. L.; FILQUEIRAS, M.; SOUZA, G. N. de; FONSECA, J. F. da. Superovulation and transcervical embryo recovery in Lacaune ewes raised under tropical conditions. Animal Reprodroduction, v. 15, n. 3, p. 402, Jul./Sept. 2018. Abstract A089. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 63. | | SOUZA-FABJAN, J. M. G.; BALARO, M. F. A.; BARBOSA, T. G. B.; FONSECA, J. F. da; FIGUEIRA, L. M.; TEIXEIRA, T. A.; CARVALHEIRA, L. R.; BRANDÃO, F. Z. GnRH potential to synchronize follicular emergence and ovulation prior to superovulatory day 0 protocol in sheep. Animal Reproduction, v. 12, n. 3, p. 712, Jul./Sept. 2015. Proceedings of the 29th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Gramado, RS, Brazil, August 20th to 23rd, 2015, and 31st Meeting of the European Embryo Transfer Association (AETE); Ghent, Belgium,
September 11th...| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 64. | | MAIA, A. L. R. S.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G.; VEIGA, M. O.; BALARO, M. F. A.; SIQUEIRA, L. G. B.; FACO, O.; FONSECA, J. F. da. Hydrometra in dairy goats: ultrasonic variables and therapeutic protocols evaluated during the reproductive season. Animal Reproduction Science, v. 197, p. 203-211, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 65. | | BRAIR, V. L.; MAIA, A. L. R. S.; CORREIA, L. F. L.; BARBOSA, N. O.; SANTOS, J. D. R.; BRANDÃO, F. Z.; FONSECA, J. F. da; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G. Gene expression patterns of in vivo-derived sheep blastocysts is more affected by vitrification than slow freezing technique. Cryobiology, v. 95, p. 110-115, 2020.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 66. | | BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA FABJAN, J. M. G. de; ALFRADIQUE, V. A. P.; CORTÊS, L. R.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. FSH dose and strategy of administration during ovarian stimulation alter the gene expression profile in ovine cumulus-oocyte complexes. Animal Reprodroduction, v. 14, n. 3, p. 746, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Cabo de Santo Agostinho, PE, Brazil, August 17 to 19, 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 67. | | VEIGA, M. de O.; MAIA, A. L. R. e S.; OLIVEIRA, V. M. de; PRATES, J. F.; BRAIR, V. L.; BARRETO, C. K.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G.; FERREIRA, M. I. C.; FONSECA, J. F. da. Indução de estro sincronizado com diferentes dispositivos vaginais em cabras da raça Saanen durante a estação de anestro. Revista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 496, jan./mar. 2017. Edição dos anais do XXII Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 68. | | TAIRA, A. R.; BRANDÃO, F. Z.; BRAIR, V. L.; COSENTINO, I. O.; LEAL, F. S. C.; RIBEIRO, A. C. S.; BALARO, M. F. A.; BATISTA, I. I. T. P.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; UNGERFELD, R. Biostimulation with the ram effect increases the follicle recruitment, ovulatory diameter, and embryo viability rate in superovulated ewes. Theriogenology, v. 181, p. 140-146, Mar. 2022.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 69. | | SOUZA-FABJAN, J. M. G. de; OLIVARES, C. C. S.; VELTRI, A. B. P.; BALARO, M. F. A.; FREITAS, V. J. de F.; FONSECA, J. F. da; BRANDÃO, F. Z. Avaliação da longevidade espermática após o uso de diferentes métodos de seleção de espermatozóides ovinos congelados. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 21., 2015, Belo Horizonte. Anais... Belo Horizonte: CBRA, 2015. p. 199.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 70. | | BRAGANÇA, G. M.; SOUZA-FABJAN, J. M. G.; BATISTA, R. I. T. P.; RIBEIRO, L. S.; BRAIR, V. L.; CÔRTES, L. R.; SOUZA, C. V.; TRAIRA, A. R.; FONSECA, J. F. da; MENCHACA, A.; BRANDÃO, F. Z. Different progestagens sources do not affect the follicular population and the morphological quality of oocytes during ovarian stimulation in Santa Inês ewes. Animal Reprodroduction, v. 15, n. 3, p. 451, Jul./Sept. 2018. Abstracts A151. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 71. | | PRELLWITZ, L.; ZAMBRINI, F. N.; GUIMARÃES, J. D.; SOUSA, M. A. P. de; ANDRADE, L. F. A. de; SOUZA-FABJAN, J. M. G.; OLIVEIRA, M. E. F. de; FONSECA, J. F. da. Donor evaluation and scoring for non-surgical embryo transfer in Santa Inês sheep. Revista Brasileira de Reprodução Animal, v. 43, n. 2, p. 484, abr./jun. 2019. Edição dos resumos do XXIII Congresso Brasileiro de Reprodução Animal, Gramado, RS, Brasil, 15 a 17 de maio 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 72. | | BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; ALFRADIQUE, V. A. P.; ARASHIRO, E. K. N.; COSENTINO, I. O.; PINTO, P. H. N.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. Dose and administration protocol for FSH used for ovarian stimulation affect gene expression in sheep cumulus-oocyte complexes. Reproduction, Fertility and Development, v. 30, n. 9, p. 1234-1244, 2018.| Biblioteca(s): Embrapa Gado de Leite. |
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| 73. | | BRAGANÇA, G. M.; ALFRADIQUE, V. A. P.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G. de; ARASHIRO, E. K. N.; BALARO, M. F. A.; OLIVEIRA, I. C. de; BRANDÃO, F. Z.; CÔRTES, L. R.; FONSECA, J. F. da. Efeito de diferentes protocolos hormonais para superestimulação ovariana sobre o número e a qualidade de oócitos em ovelhas da raça Santa Inês. Revista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 470, jan./mar. 2017. Edição dos anais do XXII Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 74. | | BATISTA, R. I. T. P.; VERGANI, G. B.; LIMA, M. S. D.; DIAS, J. H.; TEIXEIRA, D. I. A.; SILVA, K. de M.; MONTEIRO, A. W. U.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Effect of estrus synchronization and superovulation protocol on the distribution of ovine embryos at different stages of development. Revista Brasileira de Reprodução Animal, v. 43, n. 2, p. 500, abr./jun. 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 75. | | CAMPELO, I. S.; PEREIRA, A. F.; ALCÂNTARA-NETO, A. S.; CANEL, N. G.; SOUZA-FABJAN, J. M. G.; TEIXEIRA, D. I. A.; CAMARGO, L. S. de A.; MELO, L. M.; RÁDIS-BAPTISTA, G.; SALAMONE, D. F.; FREITAS, V. J. F. Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos. Zygote, v. 24, 1, p. 48-57, 2014. 10 p.| Biblioteca(s): Embrapa Gado de Leite. |
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| 76. | | SOUZA-FABJAN, J. M. G.; ROSA, R. M. da; BALARO, M. F. A.; PINTO, P. H. N.; SANTOS, G. B. dos; ARASHIRO, E. K. N.; FONSECA, J. F. da; UNGERFELD, R.; BRANDÃO, F. Z. Effect of different hormonal combinations on follicular wave emergence and superovulatory response in sheep. Theriogenology, v. 103, p. 24-29, Nov. 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 77. | | SOUZA-FABJAN, J. M. G.; BRAGANÇA, G. M.; RIBEIRO, L. dos S.; PINTO, P. H. N.; SANTOS, G. B. dos; BRAIR, V. L.; OLIVEIRA, I. C.; BATISTA, R. I. T. P.; FONSECA, J. F. da; BRANDÃO, F. Z. Efeito de diferentes doses de protocolos hormonais para superestimulação ovariana na população folicular de ovelhas da raça Santa Inês. Revista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 468, jan./mar. 2017. Edição dos anais do XXII Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 78. | | OLIVEIRA, M. E. F.; FONSECA, J. F. da; VICENTE, W. R. R.; RODRIGUES, N. N.; VERGANI, G. B.; SOUZA-FABJAN, J. M. G.; JAMIESON, M.; CRISTESCU, A.; MURAWSKI, M.; BARTLEWSKI, P. M. Are the spectral Doppler indices of ovarian arteries indicative of antral follicular development and predictive of ovulatory responses and embryo yields in superovulated ewes?. Reproductive Biology, v. 19, p. 394-403, 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 79. | | MACHADO, V. de O.; LEAL, F. S. C.; BONATO, G. C.; DIAS, J. H.; ARRAIS, A. M.; MAIA, A. L. R. e S.; CÔRTES, L. R.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Administração de hCG após a inseminação artificial eleva a taxa de gestação em cabras Saanen acíclicas submetidas a indução de estro sincronizado. Revista Brasileira de Reprodução Animal, v. 43, n. 2, p. 545, abr./jun. 2019. Edição dos resumos do XXIII Congresso Brasileiro de Reprodução Animal, Gramado, RS, Brasil, 15 a 17 de maio 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 80. | | CORTÊS, L. R.; FRAGA, M. O.; SILVA, D. S.; PRATES, J. F.; MARTINS, B. B.; FIGUEIRA, L. M.; BONATO, G. C.; ARRAIS, A. M.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; FONSECA, J. F. da. Administration of HCG seven days after estrous onset increases the pregnancy rate in Toggenburg goats subjected to induction of synchronized estrus and natural mating. Animal Reprodroduction, v. 15, n. 3, p. 366, Jul./Sept. 2018. Abstract A051. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 142 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
23/08/2017 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA FABJAN, J. M. G. de; ALFRADIQUE, V. A. P.; CORTÊS, L. R.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
Gláucia Mota Bragança, Universidade Federal Fluminense (UFF) - Rio de Janeiro, RJ; Ribrio Ivan Tavares Pereira Batista; Joanna Maria Gonçalves de Souza Fabjan; Vivian Angélico Pereira Alfradique; Luana Rangel Côrtes; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandao. |
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Título: |
FSH dose and strategy of administration during ovarian stimulation alter the gene expression profile in ovine cumulus-oocyte complexes. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Animal Reprodroduction, v. 14, n. 3, p. 746, Jul./Sept. 2017. |
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Idioma: |
Inglês |
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Notas: |
Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Cabo de Santo Agostinho, PE, Brazil, August 17 to 19, 2017. |
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Conteúdo: |
Ovarian stimulation is an important tool to increase the number of oocytes obtained by laparoscopy for the in vitro production of embryos (IVP). In sheep, different concentrations of FSH administered in single dose (SD) or multiple doses (MD) have been adopted. In parallel, the oocyte quality is fundamental for IVP success, so strategies to produce more competent oocytes have been evaluated. The aim of this study was to evaluate the gene expression profile of BCB+ COC from different hormonal protocols of ovarian stimulation in Santa Inês ewes. To achieve that, a cross-over design was used, where 12 pluriparous ewes had their follicular wave synchronized (Balaro et al., Domest Anim Endocrinol, 54: 10-14, 2016). At 80 h after progestogen implant removal, all ewes received a new vaginal sponge and it started the stimulation by administration of: 80 (Group 1 - 80-SD) or 120 (Group 2 - 120-SD) mg FSH (Folltropin-V®, Bioniche Animal Health, Ontario, Canada) and 300 IU of eCG both in single dose, or 80 (Group 3 - 80-MD) or 120 (Group 4 - 120-MD) in decreasing doses (50/30/20%) every 12 h. The COCs were recovered by laparoscopy and classified morphologically in grade I / II (homogeneous ooplasm and more than 3 cumulus cells layers), III (homogeneous ooplasm and less than 3 cumulus cells layers or partially denuded) and IV (heterogeneous ooplasm or degenerate). For inference of the development competence GI, II and III COCs were stained with bright cresyl blue (BCB) and classified into: BCB+ (competent) and BCB- (non-competent). These variables were compared by ANOVA followed by Tukey test. The abundance of mRNA that encodes proteins associated with steroidogenesis (STAR, FSHr, LHr and ER?), oocyte quality (MATER, BMP15, GDF9 and ZAR1) and apoptosis (BAX and Bcl-2) was assessed by real-time qPCR normalized with GAPDH in BCB+ COCs. The abundance of gene transcripts associated with steroidogenesis was down-regulated (P <0.05) with increasing FSH concentration, when administration was performed in a single dose (80-SD and 120-SD). On the other hand, when the administration was performed in MD, only the LHr was down-regulated (80-MD and 120-MD). In the 80-MD group, FSHr and Er? were down-regulated (P <0.05) in comparison with 80-SD. For genes related with oocyte quality, 80-MD showed up-regulation (P <0.05) to MATER (when compared to 80-SD), ZAR1 and MATER (compared to 120-SD). Nonetheless, apoptosis genes were not affected. These data demonstrate that the FSH dose and strategy of administration affect the gene expression profile in ovine COCs. Subsequent studies are necessary to assess the effect of this change on maturation rate and developmental competence. MenosOvarian stimulation is an important tool to increase the number of oocytes obtained by laparoscopy for the in vitro production of embryos (IVP). In sheep, different concentrations of FSH administered in single dose (SD) or multiple doses (MD) have been adopted. In parallel, the oocyte quality is fundamental for IVP success, so strategies to produce more competent oocytes have been evaluated. The aim of this study was to evaluate the gene expression profile of BCB+ COC from different hormonal protocols of ovarian stimulation in Santa Inês ewes. To achieve that, a cross-over design was used, where 12 pluriparous ewes had their follicular wave synchronized (Balaro et al., Domest Anim Endocrinol, 54: 10-14, 2016). At 80 h after progestogen implant removal, all ewes received a new vaginal sponge and it started the stimulation by administration of: 80 (Group 1 - 80-SD) or 120 (Group 2 - 120-SD) mg FSH (Folltropin-V®, Bioniche Animal Health, Ontario, Canada) and 300 IU of eCG both in single dose, or 80 (Group 3 - 80-MD) or 120 (Group 4 - 120-MD) in decreasing doses (50/30/20%) every 12 h. The COCs were recovered by laparoscopy and classified morphologically in grade I / II (homogeneous ooplasm and more than 3 cumulus cells layers), III (homogeneous ooplasm and less than 3 cumulus cells layers or partially denuded) and IV (heterogeneous ooplasm or degenerate). For inference of the development competence GI, II and III COCs were stained with bright cresyl blue (BCB) and classified in... Mostrar Tudo |
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Palavras-Chave: |
Embryo Technology; Expressão gênica; FSH; In vitro embryo production; Produção de embriões in vitro. |
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Thesagro: |
Ovino; Reprodução animal; Transferência de embrião. |
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Thesaurus NAL: |
Gene expression; Ova; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162972/1/cnpc-2017-FSH.pdf
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Marc: |
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100 1 $aBRAGANÇA, G. M.
245 $aFSH dose and strategy of administration during ovarian stimulation alter the gene expression profile in ovine cumulus-oocyte complexes.$h[electronic resource]
260 $aAnimal Reprodroduction, v. 14, n. 3, p. 746, Jul./Sept. 2017.$c2017
500 $aProceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Cabo de Santo Agostinho, PE, Brazil, August 17 to 19, 2017.
520 $aOvarian stimulation is an important tool to increase the number of oocytes obtained by laparoscopy for the in vitro production of embryos (IVP). In sheep, different concentrations of FSH administered in single dose (SD) or multiple doses (MD) have been adopted. In parallel, the oocyte quality is fundamental for IVP success, so strategies to produce more competent oocytes have been evaluated. The aim of this study was to evaluate the gene expression profile of BCB+ COC from different hormonal protocols of ovarian stimulation in Santa Inês ewes. To achieve that, a cross-over design was used, where 12 pluriparous ewes had their follicular wave synchronized (Balaro et al., Domest Anim Endocrinol, 54: 10-14, 2016). At 80 h after progestogen implant removal, all ewes received a new vaginal sponge and it started the stimulation by administration of: 80 (Group 1 - 80-SD) or 120 (Group 2 - 120-SD) mg FSH (Folltropin-V®, Bioniche Animal Health, Ontario, Canada) and 300 IU of eCG both in single dose, or 80 (Group 3 - 80-MD) or 120 (Group 4 - 120-MD) in decreasing doses (50/30/20%) every 12 h. The COCs were recovered by laparoscopy and classified morphologically in grade I / II (homogeneous ooplasm and more than 3 cumulus cells layers), III (homogeneous ooplasm and less than 3 cumulus cells layers or partially denuded) and IV (heterogeneous ooplasm or degenerate). For inference of the development competence GI, II and III COCs were stained with bright cresyl blue (BCB) and classified into: BCB+ (competent) and BCB- (non-competent). These variables were compared by ANOVA followed by Tukey test. The abundance of mRNA that encodes proteins associated with steroidogenesis (STAR, FSHr, LHr and ER?), oocyte quality (MATER, BMP15, GDF9 and ZAR1) and apoptosis (BAX and Bcl-2) was assessed by real-time qPCR normalized with GAPDH in BCB+ COCs. The abundance of gene transcripts associated with steroidogenesis was down-regulated (P <0.05) with increasing FSH concentration, when administration was performed in a single dose (80-SD and 120-SD). On the other hand, when the administration was performed in MD, only the LHr was down-regulated (80-MD and 120-MD). In the 80-MD group, FSHr and Er? were down-regulated (P <0.05) in comparison with 80-SD. For genes related with oocyte quality, 80-MD showed up-regulation (P <0.05) to MATER (when compared to 80-SD), ZAR1 and MATER (compared to 120-SD). Nonetheless, apoptosis genes were not affected. These data demonstrate that the FSH dose and strategy of administration affect the gene expression profile in ovine COCs. Subsequent studies are necessary to assess the effect of this change on maturation rate and developmental competence.
650 $aGene expression
650 $aOva
650 $aReproduction
650 $aSheep
650 $aOvino
650 $aReprodução animal
650 $aTransferência de embrião
653 $aEmbryo Technology
653 $aExpressão gênica
653 $aFSH
653 $aIn vitro embryo production
653 $aProdução de embriões in vitro
700 1 $aBATISTA, R. I. T. P.
700 1 $aSOUZA FABJAN, J. M. G. de
700 1 $aALFRADIQUE, V. A. P.
700 1 $aCORTÊS, L. R.
700 1 $aCAMARGO, L. S. de A.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
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Embrapa Caprinos e Ovinos (CNPC) |
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