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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
17/03/2011 |
Data da última atualização: |
15/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
POLISSENI, J.; SÁ, W. F. de; GUERRA, M. O.; MACHADO, M. A.; SERAPIÃO, R. V.; CARVALHO, B. C. de; CAMARGO, L. S. de A.; PETERS, V. M. |
Afiliação: |
JULIANA POLISSENI, UFJF; WANDERLEI FERREIRA DE SÁ, Pesquisador aposentado do CNPGL; MARTHA DE OLIVEIRA GUERRA, UFJF; MARCO ANTONIO MACHADO, CNPGL; RAQUEL VARELLA SERAPIÃO, UFJF; BRUNO CAMPOS DE CARVALHO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; VERA MARIA PETERS, UFJF. |
Título: |
Post biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Fertility and Sterility, v. 93. n. 3, p. 783-788, 2010. |
DOI: |
https://doi.org/10.1016/j.fertnstert.2008.10.023 |
Idioma: |
Inglês |
Conteúdo: |
Objective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres.Objective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres. MenosObjective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres.Objective: To evaluate the effect of the biopsy of 8-cell t... Mostrar Tudo |
Palavras-Chave: |
Embryo quality; Hatching rate; PGD; Sexing. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03686naa a2200265 a 4500 001 1881353 005 2024-02-15 008 2010 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.fertnstert.2008.10.023$2DOI 100 1 $aPOLISSENI, J. 245 $aPost biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis.$h[electronic resource] 260 $c2010 520 $aObjective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres.Objective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres. 653 $aEmbryo quality 653 $aHatching rate 653 $aPGD 653 $aSexing 700 1 $aSÁ, W. F. de 700 1 $aGUERRA, M. O. 700 1 $aMACHADO, M. A. 700 1 $aSERAPIÃO, R. V. 700 1 $aCARVALHO, B. C. de 700 1 $aCAMARGO, L. S. de A. 700 1 $aPETERS, V. M. 773 $tFertility and Sterility$gv. 93. n. 3, p. 783-788, 2010.
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Embrapa Gado de Leite (CNPGL) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
17/03/2011 |
Data da última atualização: |
04/07/2022 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
SOUZA FILHO, A. P. da S. |
Afiliação: |
ANTONIO PEDRO DA SILVA SOUZA FILHO, CPATU. |
Título: |
A biodiversidade contra as pragas. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: GORAYEB, I. de S. (Coord.). Amazônia. Belém, PA: RM Graph, O Liberal, 2010. |
Páginas: |
p. 229-231. |
Idioma: |
Português |
Thesagro: |
Agricultura; Praga. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00434naa a2200145 a 4500 001 1881350 005 2022-07-04 008 2010 bl uuuu u00u1 u #d 100 1 $aSOUZA FILHO, A. P. da S. 245 $aA biodiversidade contra as pragas.$h[electronic resource] 260 $c2010 300 $ap. 229-231. 650 $aAgricultura 650 $aPraga 773 $tIn: GORAYEB, I. de S. (Coord.). Amazônia. Belém, PA: RM Graph, O Liberal, 2010.
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