| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Milho e Sorgo. |
|
Data corrente: |
13/02/2015 |
|
Data da última atualização: |
23/05/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
FERREIRA, D. A. F. da; COTTA, S. R.; VOLLÚ, R. E.; JURELEVICIUS, D. A. de; MARQUES, J. M.; MARRIEL, I. E.; SELDIN, L. |
|
Afiliação: |
IVANILDO EVODIO MARRIEL, CNPMS. |
|
Título: |
Endophytic microbial community in two transgenic maize genotypes and in their near-isogenic non-transgenic maize genotype. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
BMC Microbiology, v. 14, n. 332, p. 1-9, 2014. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Background: Despite all the benefits assigned to the genetically modified plants, there are still no sufficient data available in literature concerning the possible effects on the microbial communities associated with these plants. Therefore, this study was aimed at examining the effects of the genetic modifications of two transgenic maize genotypes (MON810–expressing the insecticidal Bt-toxin and TC1507–expressing the insecticidal Bt-toxin and the herbicide resistance PAT [phosphinothricin-N -acetyltransferase]) on their endophytic microbial communities, in comparison to the microbial community found in the near-isogenic non-transgenic maize (control). Results: The structure of the endophytic communities (Bacteria, Archaea and fungi) and their composition (Bacteria) were evaluated by denaturing gradient gel electrophoresis (DGGE) and the construction of clone libraries, respectively. DGGE analysis and the clone libraries of the bacterial community showed that genotype TC1507 slightly differed from the other two genotypes. Genotype TC1507 showed a higher diversity within its endophytic bacterial community when compared to the other genotypes. Although some bacterial genera were found in all genotypes, such as the genera Burkholderia, Achromobacer and Stenotrophomonas, some were unique to genotype TC1507. Moreover, OTUs associated with Enterobacter predominated only in TC1507 clone libraries. Conclusion: The endophytic bacterial community of the maize genotype TC1507 differed from the communities of the maize genotype MON810 and of their near-isogenic parental genotypes (non-Bt or control). The differences observed among the maize genotypes studied may be associated with insertion of the gene coding for the protein PAT present only in the transgenic genotype TC1507. MenosBackground: Despite all the benefits assigned to the genetically modified plants, there are still no sufficient data available in literature concerning the possible effects on the microbial communities associated with these plants. Therefore, this study was aimed at examining the effects of the genetic modifications of two transgenic maize genotypes (MON810–expressing the insecticidal Bt-toxin and TC1507–expressing the insecticidal Bt-toxin and the herbicide resistance PAT [phosphinothricin-N -acetyltransferase]) on their endophytic microbial communities, in comparison to the microbial community found in the near-isogenic non-transgenic maize (control). Results: The structure of the endophytic communities (Bacteria, Archaea and fungi) and their composition (Bacteria) were evaluated by denaturing gradient gel electrophoresis (DGGE) and the construction of clone libraries, respectively. DGGE analysis and the clone libraries of the bacterial community showed that genotype TC1507 slightly differed from the other two genotypes. Genotype TC1507 showed a higher diversity within its endophytic bacterial community when compared to the other genotypes. Although some bacterial genera were found in all genotypes, such as the genera Burkholderia, Achromobacer and Stenotrophomonas, some were unique to genotype TC1507. Moreover, OTUs associated with Enterobacter predominated only in TC1507 clone libraries. Conclusion: The endophytic bacterial community of the maize genotype TC1507 differed... Mostrar Tudo |
|
Palavras-Chave: |
Enzima PAT; Proteína Cry. |
|
Thesagro: |
Alimento transgênico; Milho. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02526naa a2200241 a 4500
001 2008692
005 2017-05-23
008 2014 bl uuuu u00u1 u #d
100 1 $aFERREIRA, D. A. F. da
245 $aEndophytic microbial community in two transgenic maize genotypes and in their near-isogenic non-transgenic maize genotype.$h[electronic resource]
260 $c2014
520 $aBackground: Despite all the benefits assigned to the genetically modified plants, there are still no sufficient data available in literature concerning the possible effects on the microbial communities associated with these plants. Therefore, this study was aimed at examining the effects of the genetic modifications of two transgenic maize genotypes (MON810–expressing the insecticidal Bt-toxin and TC1507–expressing the insecticidal Bt-toxin and the herbicide resistance PAT [phosphinothricin-N -acetyltransferase]) on their endophytic microbial communities, in comparison to the microbial community found in the near-isogenic non-transgenic maize (control). Results: The structure of the endophytic communities (Bacteria, Archaea and fungi) and their composition (Bacteria) were evaluated by denaturing gradient gel electrophoresis (DGGE) and the construction of clone libraries, respectively. DGGE analysis and the clone libraries of the bacterial community showed that genotype TC1507 slightly differed from the other two genotypes. Genotype TC1507 showed a higher diversity within its endophytic bacterial community when compared to the other genotypes. Although some bacterial genera were found in all genotypes, such as the genera Burkholderia, Achromobacer and Stenotrophomonas, some were unique to genotype TC1507. Moreover, OTUs associated with Enterobacter predominated only in TC1507 clone libraries. Conclusion: The endophytic bacterial community of the maize genotype TC1507 differed from the communities of the maize genotype MON810 and of their near-isogenic parental genotypes (non-Bt or control). The differences observed among the maize genotypes studied may be associated with insertion of the gene coding for the protein PAT present only in the transgenic genotype TC1507.
650 $aAlimento transgênico
650 $aMilho
653 $aEnzima PAT
653 $aProteína Cry
700 1 $aCOTTA, S. R.
700 1 $aVOLLÚ, R. E.
700 1 $aJURELEVICIUS, D. A. de
700 1 $aMARQUES, J. M.
700 1 $aMARRIEL, I. E.
700 1 $aSELDIN, L.
773 $tBMC Microbiology$gv. 14, n. 332, p. 1-9, 2014.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Arroz e Feijão. |
|
Data corrente: |
01/12/2023 |
|
Data da última atualização: |
01/12/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 4 |
|
Autoria: |
CAVALHEIRO, S. B.; MELO, L. C.; SOUZA, T. L. P. O. de; PEREIRA, H. S. |
|
Afiliação: |
SÂMELA BEUTINGER CAVALHEIRO, UNIVERSIDADE FEDERAL DE GOIÁS; LEONARDO CUNHA MELO, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; HELTON SANTOS PEREIRA, CNPAF. |
|
Título: |
Selection of parents and segregating populations of carioca common bean resistant to Fusarium wilt. |
|
Ano de publicação: |
2023 |
|
Fonte/Imprenta: |
Semina Ciências Agrárias, v. 44, n. 2, p. 499-514, mar./abr. 2023. |
|
ISSN: |
1679-0359 |
|
DOI: |
https://doi.org/10.5433/1679-0359.2023v44n2p499 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Fusarium wilt (Fusarium oxysporum f. sp. phaseoli) is one of the most common diseases in the common bean (Phaseolus vulgaris) crop, and the most viable alternative for its control is the use of resistant cultivars. However, the number of “carioca” cultivars, the most important commercial group in Brazil, which has a good level of resistance to this disease, is still low. To obtain populations with a higher degree of resistance, seven parents with different levels of resistance to Fusarium and which produce carioca grains were crossed in a complete diallel design. The 21 segregating populations obtained were evaluated in the years/generations 2012/F3, 2013/F4, and 2014/F5 in experiments in Santo Antônio de Goiás, GO, in the winter crop season (sowing in May), together with the check cultivars BRS Notável (resistant) and BRS Cometa (susceptible) in an area naturally infested with Fusarium oxysporum f. sp. phaseoli, under a center pivot irrigation system. Reaction to Fusarium wilt, yield, and 100-seed weight were evaluated. Additive and non-additive effects contributed to control of reaction to Fusarium wilt, yield, and 100-seed weight. The statistically negative general combining ability (ĝ) estimates for BRS Notável (-0.51), CNFC 15872 (-0.20), IPR Juriti (-0.18), and BRSMG Talismã (-0.13) indicate that these parents are recommended for obtaining populations more resistant to Fusarium wilt. Among them, cultivar BRS Notável also showed a statistically positive ĝ estimate for grain yield (87.90). Among the parents recommended for resistance to Fusarium wilt, cultivars IPR Juriti (0.99) and BRS Notável (0.23) are those that most contributed to obtaining populations with larger-sized beans. Cultivar BRS Notável is the most promising parent for participation in new crossing blocks, showing non-zero ĝi estimates for all traits, favoring an increase in resistance to Fusarium wilt, yield, and 100-seed weight. Populations BRS Notável / CNFC 15872, BRS Ametista / BRS Notável, BRS Notável / BRSMG Talismã, and BRS Ametista / BRSMG Talismã showed good mean values for reaction to Fusarium wilt, yield, and 100-seed weight and at least one parent with a good general combining ability estimate for resistance to Fusarium wilt. These four populations are promising for obtaining lines with superior performance for resistance to Fusarium wilt, yield, and larger-sized grains. MenosFusarium wilt (Fusarium oxysporum f. sp. phaseoli) is one of the most common diseases in the common bean (Phaseolus vulgaris) crop, and the most viable alternative for its control is the use of resistant cultivars. However, the number of “carioca” cultivars, the most important commercial group in Brazil, which has a good level of resistance to this disease, is still low. To obtain populations with a higher degree of resistance, seven parents with different levels of resistance to Fusarium and which produce carioca grains were crossed in a complete diallel design. The 21 segregating populations obtained were evaluated in the years/generations 2012/F3, 2013/F4, and 2014/F5 in experiments in Santo Antônio de Goiás, GO, in the winter crop season (sowing in May), together with the check cultivars BRS Notável (resistant) and BRS Cometa (susceptible) in an area naturally infested with Fusarium oxysporum f. sp. phaseoli, under a center pivot irrigation system. Reaction to Fusarium wilt, yield, and 100-seed weight were evaluated. Additive and non-additive effects contributed to control of reaction to Fusarium wilt, yield, and 100-seed weight. The statistically negative general combining ability (ĝ) estimates for BRS Notável (-0.51), CNFC 15872 (-0.20), IPR Juriti (-0.18), and BRSMG Talismã (-0.13) indicate that these parents are recommended for obtaining populations more resistant to Fusarium wilt. Among them, cultivar BRS Notável also showed a statistically positive ĝ estimate for g... Mostrar Tudo |
|
Palavras-Chave: |
Dialelo completo; Massa de 100 grãos. |
|
Thesagro: |
Feijão; Fusarium Oxysporum; Melhoramento Genético Vegetal; Murcha de Fusarium; Phaseolus Vulgaris; Produtividade. |
|
Thesaurus NAL: |
Beans; Plant breeding; Yields. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1159042/1/semina-2023.pdf
|
|
Marc: |
LEADER 03376naa a2200313 a 4500
001 2159042
005 2023-12-01
008 2023 bl uuuu u00u1 u #d
022 $a1679-0359
024 7 $ahttps://doi.org/10.5433/1679-0359.2023v44n2p499$2DOI
100 1 $aCAVALHEIRO, S. B.
245 $aSelection of parents and segregating populations of carioca common bean resistant to Fusarium wilt.$h[electronic resource]
260 $c2023
520 $aFusarium wilt (Fusarium oxysporum f. sp. phaseoli) is one of the most common diseases in the common bean (Phaseolus vulgaris) crop, and the most viable alternative for its control is the use of resistant cultivars. However, the number of “carioca” cultivars, the most important commercial group in Brazil, which has a good level of resistance to this disease, is still low. To obtain populations with a higher degree of resistance, seven parents with different levels of resistance to Fusarium and which produce carioca grains were crossed in a complete diallel design. The 21 segregating populations obtained were evaluated in the years/generations 2012/F3, 2013/F4, and 2014/F5 in experiments in Santo Antônio de Goiás, GO, in the winter crop season (sowing in May), together with the check cultivars BRS Notável (resistant) and BRS Cometa (susceptible) in an area naturally infested with Fusarium oxysporum f. sp. phaseoli, under a center pivot irrigation system. Reaction to Fusarium wilt, yield, and 100-seed weight were evaluated. Additive and non-additive effects contributed to control of reaction to Fusarium wilt, yield, and 100-seed weight. The statistically negative general combining ability (ĝ) estimates for BRS Notável (-0.51), CNFC 15872 (-0.20), IPR Juriti (-0.18), and BRSMG Talismã (-0.13) indicate that these parents are recommended for obtaining populations more resistant to Fusarium wilt. Among them, cultivar BRS Notável also showed a statistically positive ĝ estimate for grain yield (87.90). Among the parents recommended for resistance to Fusarium wilt, cultivars IPR Juriti (0.99) and BRS Notável (0.23) are those that most contributed to obtaining populations with larger-sized beans. Cultivar BRS Notável is the most promising parent for participation in new crossing blocks, showing non-zero ĝi estimates for all traits, favoring an increase in resistance to Fusarium wilt, yield, and 100-seed weight. Populations BRS Notável / CNFC 15872, BRS Ametista / BRS Notável, BRS Notável / BRSMG Talismã, and BRS Ametista / BRSMG Talismã showed good mean values for reaction to Fusarium wilt, yield, and 100-seed weight and at least one parent with a good general combining ability estimate for resistance to Fusarium wilt. These four populations are promising for obtaining lines with superior performance for resistance to Fusarium wilt, yield, and larger-sized grains.
650 $aBeans
650 $aPlant breeding
650 $aYields
650 $aFeijão
650 $aFusarium Oxysporum
650 $aMelhoramento Genético Vegetal
650 $aMurcha de Fusarium
650 $aPhaseolus Vulgaris
650 $aProdutividade
653 $aDialelo completo
653 $aMassa de 100 grãos
700 1 $aMELO, L. C.
700 1 $aSOUZA, T. L. P. O. de
700 1 $aPEREIRA, H. S.
773 $tSemina Ciências Agrárias$gv. 44, n. 2, p. 499-514, mar./abr. 2023.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
