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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
11/07/2014 |
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Data da última atualização: |
20/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CRISPINO, C. C.; LEMOS, S. G.; KANOGAWA, M. Y.; NOGUEIRA, A. R. de A. |
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Afiliação: |
CARLA CRIPA CRISPINO, CNTRO DE ENERGIA NUCLEAR/PIRACICABA; SHERLAN GUIMARÃES LEMOS, UNIVERSIDADE FEDERAL DA PARAÍBA, JOÃO PESSOA; MARCOS YASSUO KAMOGAWA, ESALQ/PIRACICABA; ANA RITA DE ARAUJO NOGUEIRA, CPPSE. |
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Título: |
Simultaneous determination of arsenic, antimony and selenium in agronomic samples by hydride generation and optical emission spectrometry. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Brazilian Journal of Analitical Chemistry, v. 10, p. 460-467, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
A continuous-fl ow hydride generation system was developed and used with inductively coupled plasma optical emission spectrometry (ICP OES) for simultaneous determination of As, Sb and Se in agronomic samples. A factorial design was applied in order to establish the best chemical and instrumental conditions. The factors chosen for evaluation were: reagent concentration, plasma power, carrier gas fl ow rate, and the viewing height of the plasma. The LODs of the method were 0.124, 0.168, and 0.141 ?g L-1 for As, Sb, and Se, respectively. The RSD (relative standard derivation) was lower than 1.8%, and the sampling throughput was 40 samples h-1 . To check the method accuracy, eight different kinds of agronomic samples - for- age, corn silage, mineral salt, and bovine-derived samples (blood, viscera, carcass, meat, and semen) - were spiked with different analyte concentrations (at the ?g L-1 level). Recoveries ranged from 90 to 102%. |
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Palavras-Chave: |
Agronomic sample; Factorial design; Hydride generation; Inductively coupled; Spectrometry. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/104956/1/PROCI-2014.00034.pdf
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Marc: |
LEADER 01661naa a2200217 a 4500
001 1990422
005 2023-03-20
008 2013 bl uuuu u00u1 u #d
100 1 $aCRISPINO, C. C.
245 $aSimultaneous determination of arsenic, antimony and selenium in agronomic samples by hydride generation and optical emission spectrometry.$h[electronic resource]
260 $c2013
520 $aA continuous-fl ow hydride generation system was developed and used with inductively coupled plasma optical emission spectrometry (ICP OES) for simultaneous determination of As, Sb and Se in agronomic samples. A factorial design was applied in order to establish the best chemical and instrumental conditions. The factors chosen for evaluation were: reagent concentration, plasma power, carrier gas fl ow rate, and the viewing height of the plasma. The LODs of the method were 0.124, 0.168, and 0.141 ?g L-1 for As, Sb, and Se, respectively. The RSD (relative standard derivation) was lower than 1.8%, and the sampling throughput was 40 samples h-1 . To check the method accuracy, eight different kinds of agronomic samples - for- age, corn silage, mineral salt, and bovine-derived samples (blood, viscera, carcass, meat, and semen) - were spiked with different analyte concentrations (at the ?g L-1 level). Recoveries ranged from 90 to 102%.
653 $aAgronomic sample
653 $aFactorial design
653 $aHydride generation
653 $aInductively coupled
653 $aSpectrometry
700 1 $aLEMOS, S. G.
700 1 $aKANOGAWA, M. Y.
700 1 $aNOGUEIRA, A. R. de A.
773 $tBrazilian Journal of Analitical Chemistry$gv. 10, p. 460-467, 2013.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
03/03/2010 |
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Data da última atualização: |
26/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CARVALHO, F. M.; SOUZA, R. C.; BARCELLOS, F. G.; HUNGRIA, M.; VASCONCELOS, A. T. R. |
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Afiliação: |
FABÍOLA M. CARVALHO, Laboratório Nacional de Computação Científica; RANGEL C. SOUZA, Laboratório Nacional de Computação Científica; FERNANDO G. BARCELLOS, INMETRO; MARIANGELA HUNGRIA DA CUNHA, CNPSo; ANA TEREZA R. VASCONCELOS, Laboratório Nacional de Bioinformática / INMETRO. |
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Título: |
Genomic and evolutionary comparisons of diazotrophic and pathogenic bacteria of the order Rhizobiales. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
BMC Microbiology, London, v. 10, n. 37, p. 1-15, Feb. 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Species belonging to the Rhizobiales are intriguing and extensively researched for including both bacteria with the ability to fix nitrogen when in symbiosis with leguminous plants and pathogenic bacteria to animals and plants. Similarities between the strategies adopted by pathogenic and symbiotic Rhizobiales have been described, as well as high variability related to events of horizontal gene transfer. Although it is well known that chromosomal rearrangements, mutations and horizontal gene transfer influence the dynamics of bacterial genomes, in Rhizobiales, the scenario that determine pathogenic or symbiotic lifestyle are not clear and there are very few studies of comparative genomic between these classes of prokaryotic microorganisms trying to delineate the evolutionary characterization of symbiosis and pathogenesis. Results: Non-symbiotic nitrogen-fixing bacteria and bacteria involved in bioremediation closer to symbionts and pathogens in study may assist in the origin and ancestry genes and the gene flow occurring in Rhizobiales. The genomic comparisons of 19 species of Rhizobiales, including nitrogen-fixing, bioremediators and pathogens resulted in 33 common clusters to biological nitrogen fixation and pathogenesis, 15 clusters exclusive to all nitrogen-fixing bacteria and bacteria involved in bioremediation, 13 clusters found in only some nitrogen-fixing and bioremediation bacteria, 01 cluster exclusive to some symbionts, and 01 cluster found only in some pathogens analyzed. In BBH performed to all strains studied, 77 common genes were obtained, 17 of which were related to biological nitrogen fixation and pathogenesis. Phylogenetic reconstructions for Fix, Nif, Nod, Vir, and Trb showed possible horizontal gene transfer events, grouping species of different phenotypes. Conclusions: The presence of symbiotic and virulence genes in both pathogens and symbionts does not seem to be the only determinant factor for lifestyle evolution in these microorganisms, although they may act in common stages of host infection. The phylogenetic analysis for many distinct operons involved in these processes emphasizes the relevance of horizontal gene transfer events in the symbiotic and pathogenic similarity. MenosBackground: Species belonging to the Rhizobiales are intriguing and extensively researched for including both bacteria with the ability to fix nitrogen when in symbiosis with leguminous plants and pathogenic bacteria to animals and plants. Similarities between the strategies adopted by pathogenic and symbiotic Rhizobiales have been described, as well as high variability related to events of horizontal gene transfer. Although it is well known that chromosomal rearrangements, mutations and horizontal gene transfer influence the dynamics of bacterial genomes, in Rhizobiales, the scenario that determine pathogenic or symbiotic lifestyle are not clear and there are very few studies of comparative genomic between these classes of prokaryotic microorganisms trying to delineate the evolutionary characterization of symbiosis and pathogenesis. Results: Non-symbiotic nitrogen-fixing bacteria and bacteria involved in bioremediation closer to symbionts and pathogens in study may assist in the origin and ancestry genes and the gene flow occurring in Rhizobiales. The genomic comparisons of 19 species of Rhizobiales, including nitrogen-fixing, bioremediators and pathogens resulted in 33 common clusters to biological nitrogen fixation and pathogenesis, 15 clusters exclusive to all nitrogen-fixing bacteria and bacteria involved in bioremediation, 13 clusters found in only some nitrogen-fixing and bioremediation bacteria, 01 cluster exclusive to some symbionts, and 01 cluster found only in som... Mostrar Tudo |
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Thesagro: |
Bacteria; Fixação de nitrogênio. |
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Thesaurus NAL: |
Nitrogen fixation; Nitrogen-fixing bacteria. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://www.biomedcentral.com/content/pdf/1471-2180-10-37.pdf
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Marc: |
LEADER 02905naa a2200217 a 4500
001 1659501
005 2017-07-26
008 2010 bl --- 0-- u #d
100 1 $aCARVALHO, F. M.
245 $aGenomic and evolutionary comparisons of diazotrophic and pathogenic bacteria of the order Rhizobiales.
260 $c2010
520 $aBackground: Species belonging to the Rhizobiales are intriguing and extensively researched for including both bacteria with the ability to fix nitrogen when in symbiosis with leguminous plants and pathogenic bacteria to animals and plants. Similarities between the strategies adopted by pathogenic and symbiotic Rhizobiales have been described, as well as high variability related to events of horizontal gene transfer. Although it is well known that chromosomal rearrangements, mutations and horizontal gene transfer influence the dynamics of bacterial genomes, in Rhizobiales, the scenario that determine pathogenic or symbiotic lifestyle are not clear and there are very few studies of comparative genomic between these classes of prokaryotic microorganisms trying to delineate the evolutionary characterization of symbiosis and pathogenesis. Results: Non-symbiotic nitrogen-fixing bacteria and bacteria involved in bioremediation closer to symbionts and pathogens in study may assist in the origin and ancestry genes and the gene flow occurring in Rhizobiales. The genomic comparisons of 19 species of Rhizobiales, including nitrogen-fixing, bioremediators and pathogens resulted in 33 common clusters to biological nitrogen fixation and pathogenesis, 15 clusters exclusive to all nitrogen-fixing bacteria and bacteria involved in bioremediation, 13 clusters found in only some nitrogen-fixing and bioremediation bacteria, 01 cluster exclusive to some symbionts, and 01 cluster found only in some pathogens analyzed. In BBH performed to all strains studied, 77 common genes were obtained, 17 of which were related to biological nitrogen fixation and pathogenesis. Phylogenetic reconstructions for Fix, Nif, Nod, Vir, and Trb showed possible horizontal gene transfer events, grouping species of different phenotypes. Conclusions: The presence of symbiotic and virulence genes in both pathogens and symbionts does not seem to be the only determinant factor for lifestyle evolution in these microorganisms, although they may act in common stages of host infection. The phylogenetic analysis for many distinct operons involved in these processes emphasizes the relevance of horizontal gene transfer events in the symbiotic and pathogenic similarity.
650 $aNitrogen fixation
650 $aNitrogen-fixing bacteria
650 $aBacteria
650 $aFixação de nitrogênio
700 1 $aSOUZA, R. C.
700 1 $aBARCELLOS, F. G.
700 1 $aHUNGRIA, M.
700 1 $aVASCONCELOS, A. T. R.
773 $tBMC Microbiology, London$gv. 10, n. 37, p. 1-15, Feb. 2010.
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