Data/hora: 02/06/2024 02:28:22

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 16/06/2016
Data da última atualização: | 29/12/2017
Autoria: | CASTRO, B. G.; SOUZA, M. M. S.; REGUA-MANGIA, A. H.; BITTENCOURT, A. J.
Afiliação: | BRUNO G. CASTRO, UFMT; MILIANE M. S. SOUZA, UFRRJ; ADRIANA H. REGUA-MANGIA, FIOCRUZ; AVELINO J. BITTENCOURT, UFRRJ.
Título: | Genetic relationship between Escherichia coli strains isolated from dairy mastitis and from the stable fly Stomoxys calcitrans.
Ano de publicação: | 2016
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 6, p. 479-484, junho. 2016.
Idioma: | Inglês
Conteúdo: | The stable fly Stomoxys calcitrans (Linnaeus, 1758) has been described as a potential spreader of infectious agents to cattle herds. Among the agents transmitted by this fly, Escherichia coli has attracted attention due to its potential to cause gastrointestinal disorders as well as environmental mastitis in dairy cows. Therefore, the aim of this study was to isolate and to assess the genetic diversity and the clonal relatedness among E. coli isolates from the milk of dairy mastitis and from stable flies anatomical sites by the Random Amplification of Polymorphic DNA (RAPD-PCR) technique. The molecular typing revealed a high degree of genetic polymorphism suggesting that these microorganisms have a non-clonal origin. Identical electrophoretic profiles were observed between E. coli isolates from different flies, different mammary quarters of the same cow and from cows on a single farm. These results reveal the circulation of the same bacterial lineages and suggest the role of the stable fly in bacterial dispersion. Considering the high pathogenic potential of this bacterial species, our findings alert to a more effective health surveillance.
Palavras-Chave: | Amplificação rondômica do DNA; Clonal species; Moscas dos estábulos; Random amplification of polymorphic DNA; RAPDA; Stable fly.
Thesagro: | Clone; Stomoxys Calcitrans.
Thesaurus Nal: | Milk.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/144465/1/Genetic-relationship.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (59639-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS