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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
11/09/2023 |
|
Data da última atualização: |
11/09/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 3 |
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Autoria: |
KLAFKE, K.; SANCHES, M. M.; SIHLER, W.; SOUZA, M. L. de; TONSO, A. |
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Afiliação: |
KARINA KLAFKE, UNIVERSIDADE DE SÃO PAULO; MARCIO MARTINELLO SANCHES, CNPGC; WILLIAM SIHLER, Cenargen; MARLINDA LOBO DE SOUZA, Cenargen; ALDO TONSO, UNIVERSIDADE DE SÃO PAULO. |
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Título: |
Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Pathogens, v. 12, article 1001, 2023. |
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DOI: |
https:// doi.org/10.3390/pathogens12081001 |
|
Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT - Spodoptera frugiperda (fall armyworm) is one of the most important maize pests in the world and the baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a natural pathogen of this pest, has been used as a biopesticide for its control. At present, in vivo strategies at the commercial scale are employed by multiplying the virus in the host insect in biofactory facilities; however, in vitro large-scale production is an interesting alternative to overcome the limitations of baculoviruses massal production. This study aimed to develop the process of the SfMNPV in vitro production by evaluating the effects of different multiplicities of infection (MOI) and nutritional supplements, morphological and molecular analysis of the infection on the growth of Sf9 cells and virus production. The Bioreactor Stirred Tank Reactor (STR) approach with glutamine-supplemented Sf-900 III serum free culture medium, combined with the MOI of 1.0, showed the best viral production performance, with a specific productivity above 300 occlusion bodies (OBs)/cell and volumetric productivity of 9.0 x 10¹¹ OBs/L. |
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Palavras-Chave: |
BIOPESTICIDA. |
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Thesagro: |
Controle Biológico; Spodoptera Frugiperda. |
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Thesaurus NAL: |
Biological control; Biopesticides; Spodoptera; Spodoptera frugiperda multiple nucleopolyhedrovirus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156592/1/Bioreactor-production-process-spodoptera-2023.pdf
|
|
Marc: |
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001 2156592
005 2023-09-11
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps:// doi.org/10.3390/pathogens12081001$2DOI
100 1 $aKLAFKE, K.
245 $aBioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide.$h[electronic resource]
260 $c2023
520 $aABSTRACT - Spodoptera frugiperda (fall armyworm) is one of the most important maize pests in the world and the baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a natural pathogen of this pest, has been used as a biopesticide for its control. At present, in vivo strategies at the commercial scale are employed by multiplying the virus in the host insect in biofactory facilities; however, in vitro large-scale production is an interesting alternative to overcome the limitations of baculoviruses massal production. This study aimed to develop the process of the SfMNPV in vitro production by evaluating the effects of different multiplicities of infection (MOI) and nutritional supplements, morphological and molecular analysis of the infection on the growth of Sf9 cells and virus production. The Bioreactor Stirred Tank Reactor (STR) approach with glutamine-supplemented Sf-900 III serum free culture medium, combined with the MOI of 1.0, showed the best viral production performance, with a specific productivity above 300 occlusion bodies (OBs)/cell and volumetric productivity of 9.0 x 10¹¹ OBs/L.
650 $aBiological control
650 $aBiopesticides
650 $aSpodoptera
650 $aSpodoptera frugiperda multiple nucleopolyhedrovirus
650 $aControle Biológico
650 $aSpodoptera Frugiperda
653 $aBIOPESTICIDA
700 1 $aSANCHES, M. M.
700 1 $aSIHLER, W.
700 1 $aSOUZA, M. L. de
700 1 $aTONSO, A.
773 $tPathogens$gv. 12, article 1001, 2023.
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Embrapa Gado de Corte (CNPGC) |
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