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| 1. |  | SOUZA, L. N. B. de; DIAS, N. D. S. C.; SANTANA, V. de O.; SILVEIRA, L. A.; MEIRA, M. R.; SANTOS, E. S. L. dos; FALEIRO, F. G.; CERQUEIRA-SILVA, C. B. M. Amplification test and selection of markers analogue to resistance genes in species and commercial varieties of Passiflora spp. Multi-Science Journal, v. 3, n. 1, 2020. p. 61-75| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 2 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
28/12/2020 |
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Data da última atualização: |
28/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
DIAS, N. D. S. C.; SOUZA, L. N. B. de; SANTANA, V. de O.; MEIRA, M. R.; SANTOS, E. S. L.; FALEIRO, F. G.; SILVA, C. B. M. C. |
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Afiliação: |
FABIO GELAPE FALEIRO, CPAC. |
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Título: |
Characterization and selection of ISSR molecular markers in species of Passiflora spp. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Multi-Science Journal, v. 3, n. 3.2020. |
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ISSN: |
2359-6902 |
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Idioma: |
Português |
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Conteúdo: |
The objective to be achieved with the search for molecular markers of low cost and good reproducibility for the selection and genetic characterization of passion fruit was to select ISSR (Inter Simple Sequence Repeat) primers for different unimproved varieties and cultivars of Passiflora, in order to subsidize molecular genetic studies. To achieve this goal, 23 ISSR starters were tested for eight unimproved varieties and 12 cultivars of Passiflora spp. The amplifications were obtained by electrophoresis on 2% agarose gel, photocumented under transilluminator with ultraviolet light. The ISSR primers showed satisfactory results. Among the 23 primers, 16 obtained values above 60% of efficiency when classified as good (presence of two marks or more). As a consequence of the results presented, it can be attested that such primers are suitable for studies of polymorphism, structure and genetic diversity in species of the Passiflora genus, in addition to assisting conservation and breeding programs. |
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Thesagro: |
Maracujá; Marcador Molecular; Passifloraceae; Polimorfismo Genético. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219727/1/FALEIRO-Characterization-and-selection-of-ISSR.pdf
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Marc: |
LEADER 01737naa a2200253 a 4500
001 2128773
005 2020-12-28
008 2020 bl uuuu u00u1 u #d
022 $a2359-6902
100 1 $aDIAS, N. D. S. C.
245 $aCharacterization and selection of ISSR molecular markers in species of Passiflora spp.$h[electronic resource]
260 $c2020
520 $aThe objective to be achieved with the search for molecular markers of low cost and good reproducibility for the selection and genetic characterization of passion fruit was to select ISSR (Inter Simple Sequence Repeat) primers for different unimproved varieties and cultivars of Passiflora, in order to subsidize molecular genetic studies. To achieve this goal, 23 ISSR starters were tested for eight unimproved varieties and 12 cultivars of Passiflora spp. The amplifications were obtained by electrophoresis on 2% agarose gel, photocumented under transilluminator with ultraviolet light. The ISSR primers showed satisfactory results. Among the 23 primers, 16 obtained values above 60% of efficiency when classified as good (presence of two marks or more). As a consequence of the results presented, it can be attested that such primers are suitable for studies of polymorphism, structure and genetic diversity in species of the Passiflora genus, in addition to assisting conservation and breeding programs.
650 $aMaracujá
650 $aMarcador Molecular
650 $aPassifloraceae
650 $aPolimorfismo Genético
700 1 $aSOUZA, L. N. B. de
700 1 $aSANTANA, V. de O.
700 1 $aMEIRA, M. R.
700 1 $aSANTOS, E. S. L.
700 1 $aFALEIRO, F. G.
700 1 $aSILVA, C. B. M. C.
773 $tMulti-Science Journal$gv. 3, n. 3.2020.
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Embrapa Cerrados (CPAC) |
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