Data/hora: 10/06/2024 02:27:13

Biblioteca(s): | Embrapa Rondônia.
Data corrente: | 30/12/2021
Data da última atualização: | 01/01/2022
Autoria: | SOUZA, J. A. de; SANTOS, M. R. A. dos.
Afiliação: | JAQUELINE ANDRUCHEVITZ DE SOUZA, FIMCA; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO.
Título: | In vitro propagation of Piper hispidum by somatic embryogenesis in leaf explants.
Ano de publicação: | 2021
Fonte/Imprenta: | International Journal of Development Research, v. 11, n. 12, p. 52392-52393, Dec. 2021.
ISSN: | 2230-9926
DOI: | https://doi.org/10.37118/ijdr.23567.12.2021
Idioma: | Inglês
Conteúdo: | A protocol for regeneration of P. hispidum through somatic embryogenesis in leaf explants is described. Under aseptic conditions, the leaves were cut into 1.0 cm2 explants. These explants were inoculated into test tubes with 10.0 mL of an Murashige and Skoog (MS) basal culture medium supplemented with 30.0 g L-1 sucrose, 6.0 g L-1 agar and a factorial combination of the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) and 6-Benzylaminopurine (BA), both at 0.0, 1.0, 2.0, 3.0 and 4.0 mg L-1 , totalizing 25 treatments. The pH was adjusted to 5.8 and the medium autoclaved at 121°C for 20 minutes. After 75 days, the average number of cotyledonary somatic embryos in each explant was recorded.Maximum number of 9.2 cotyledonary embryos per explant was observed with the combination of 2.0 mg L-1 BA + 2.0 mg L-1 2,4-D. The subculture of the cotyledonary embryos to a medium without growth regulators resulted in 95% conversion into plantlets, which were acclimatized with 100% survival.
Palavras-Chave: | Cotyledonary embryos; Embriogênese somática; Protocolo de regeneração.
Thesagro: | Cultura de Tecido; Cultura In Vitro; Explante; Folha; Propagação Vegetativa; Reprodução Vegetal.
Thesaurus Nal: | Explants; Genetic techniques and protocols; In vitro culture; Leaves; Piper hispidum; Plant reproduction; Somatic embryogenesis; Tissue culture; Vegetative propagation.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/229831/1/cpafro-18666.pdf
Registro original: | Embrapa Rondônia (CPAF-RO)
Exemplares: | CPAF-RO - (18666-1) - UPC - AP - DD -  -  -  - DIS