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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
25/08/2020 |
Data da última atualização: |
25/08/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LEASTRO, M. O.; CASTRO, D. Y. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. |
Afiliação: |
MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico; DEIBIS YORLENIS ORTEGA CASTRO, Universidad Politécnica de Valencia; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, Universidad Politécnica de Valencia; JESÚS ÁNGEL SÁNCHEZ-NAVARRO, Universidad Politécnica de Valencia. |
Título: |
Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Frontiers in Microbiology, June 2020. |
ISSN: |
1664-302X |
DOI: |
https://doi.org/10.3389/fmicb.2020.01231 |
Idioma: |
Inglês |
Conteúdo: |
Citrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only the trans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting that p61 could also activate a plant defense response mechanism. This is the first report describing the RSS activity for CiLV-C proteins and, moreover, for a member of the family Kitaviridae. MenosCitrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only the trans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting th... Mostrar Tudo |
Thesagro: |
Doença de Planta; Fruta Cítrica. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02381naa a2200229 a 4500 001 2124547 005 2020-08-25 008 2020 bl uuuu u00u1 u #d 022 $a1664-302X 024 7 $ahttps://doi.org/10.3389/fmicb.2020.01231$2DOI 100 1 $aLEASTRO, M. O. 245 $aCitrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity.$h[electronic resource] 260 $c2020 520 $aCitrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only the trans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting that p61 could also activate a plant defense response mechanism. This is the first report describing the RSS activity for CiLV-C proteins and, moreover, for a member of the family Kitaviridae. 650 $aDoença de Planta 650 $aFruta Cítrica 700 1 $aCASTRO, D. Y. O. 700 1 $aASTUA, J. de F. 700 1 $aKITAJIMA, E. W. 700 1 $aPALLÁS, V. 700 1 $aSÁNCHEZ-NAVARRO, J. Á. 773 $tFrontiers in Microbiology, June 2020.
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
09/11/2021 |
Data da última atualização: |
09/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 2 |
Autoria: |
SILVA, R. F. da; SILVA, R. M. da; LIMA, S. S. de; SOUZA, J. R. M. de; SOUZA, J. K. M. de; RIBEIRO, G. T.; CHAER, G. M. |
Afiliação: |
RAFAELA MARTINS DA SILVA, UFV; RAKIELY MARTINS DA SILVA, UENF; SANDRA SANTANA DE LIMA, UFRRJ; JIANNE RAFAELA MAZZINI DE SOUZA, UFV; JHENY KESLEY MAZZINI DE SOUZA, UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL REI; GILBERTO TERRA RIBEIRO, Sucupira Agroforests; GUILHERME MONTANDON CHAER, CNPAB. |
Título: |
Soil macrofauna as a bioindicator of soil quality in successional agroforestry systems. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Research, Society and Developmentv. 10, n. 10, e580101019144, 2021. |
ISSN: |
2525-3409 |
DOI: |
https://doi.org/10.33448/rsd-v10i10.19144 |
Idioma: |
Inglês |
Conteúdo: |
he objective of this study was to evaluate soil macrofauna as a bioindicator of soil quality in successional agroforestry systems and secondary forests. The study was conducted in the southern lower region of Bahia in Brazil, in two areas: a successional agroforestry system (AFS18) and native forest (NF). AFS18 consists of two species: mahogany (Khaya ivorensis and Khaya grandifoliola), açaí (Euterpe oleracea), cacao (Theobroma cacau) and banana (Musa spp.). Sampling was carried out in the dry (June) and rainy (October) seasons of 2019, and eight soil monoliths were collected in both areas. A total of 889 individuals from the soil macrofauna were sampled. The highest frequency (RF) of taxons occurred in NF in the rainy season, and the groups that stood out were: Oligochaeta with 42% FR in ASF18, Formicide with 33.9% in NF and Isoptera with 58% in AFS18. The macrofauna structure of the soil varied according to the time of collection. The density of macrofauna individuals differed between areas only in the dry season. The highest number of ind.m² was observed in the area NF (378) when compared to ASF18 (196). TOC, Mg2+, Al3+ and CTC were related to AF on both occasions of collection and AFS18 in the rainy season, K+, P and pH were associated with AFS18 in the dry season. The diversity, equitability and richness of the soil macrofauna was greater in AF area. HFA18 in the rainy season was similar to NF, favoring colonization of the area by soil macrofauna organisms. |
Palavras-Chave: |
Biological indicator; Soil recovery. |
Thesaurus NAL: |
Biodiversity; Soil fauna. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
Marc: |
LEADER 02297naa a2200265 a 4500 001 2135954 005 2021-11-09 008 2021 bl uuuu u00u1 u #d 022 $a2525-3409 024 7 $ahttps://doi.org/10.33448/rsd-v10i10.19144$2DOI 100 1 $aSILVA, R. F. da 245 $aSoil macrofauna as a bioindicator of soil quality in successional agroforestry systems.$h[electronic resource] 260 $c2021 520 $ahe objective of this study was to evaluate soil macrofauna as a bioindicator of soil quality in successional agroforestry systems and secondary forests. The study was conducted in the southern lower region of Bahia in Brazil, in two areas: a successional agroforestry system (AFS18) and native forest (NF). AFS18 consists of two species: mahogany (Khaya ivorensis and Khaya grandifoliola), açaí (Euterpe oleracea), cacao (Theobroma cacau) and banana (Musa spp.). Sampling was carried out in the dry (June) and rainy (October) seasons of 2019, and eight soil monoliths were collected in both areas. A total of 889 individuals from the soil macrofauna were sampled. The highest frequency (RF) of taxons occurred in NF in the rainy season, and the groups that stood out were: Oligochaeta with 42% FR in ASF18, Formicide with 33.9% in NF and Isoptera with 58% in AFS18. The macrofauna structure of the soil varied according to the time of collection. The density of macrofauna individuals differed between areas only in the dry season. The highest number of ind.m² was observed in the area NF (378) when compared to ASF18 (196). TOC, Mg2+, Al3+ and CTC were related to AF on both occasions of collection and AFS18 in the rainy season, K+, P and pH were associated with AFS18 in the dry season. The diversity, equitability and richness of the soil macrofauna was greater in AF area. HFA18 in the rainy season was similar to NF, favoring colonization of the area by soil macrofauna organisms. 650 $aBiodiversity 650 $aSoil fauna 653 $aBiological indicator 653 $aSoil recovery 700 1 $aSILVA, R. M. da 700 1 $aLIMA, S. S. de 700 1 $aSOUZA, J. R. M. de 700 1 $aSOUZA, J. K. M. de 700 1 $aRIBEIRO, G. T. 700 1 $aCHAER, G. M. 773 $tResearch, Society and Developmentv. 10$gn. 10, e580101019144, 2021.
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