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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
14/05/2004 |
Data da última atualização: |
14/05/2004 |
Autoria: |
SALLES, J. F.; SOUZA, F. A. de; VAN ELSAS, J. D. |
Título: |
Molecular method to assess the diversity of Burkholderia species in environmental. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 68, n.4, p. 1595-1603, 2002 |
Idioma: |
Inglês |
Conteúdo: |
In spite of the importance of many members of the genus Burkholderia in the soil microbial community, no direct method to assess the diversity of this genus has been developed so far. The aim of this work was the development of soil DNA-based PCR-denaturing gradient gel electrophoresis (DGGE), a powerful tool for studying the diversity of microbial communities, for detection and analysis of the Burkholderia diversity in soil samples. Primers specific for the genus Burkholderia were developed based on the 16S rDNA gene sequence and were evaluated in PCRs performed with genomic DNAs from Burkholderia and non-Burkholderia species as the templates. The primer system used exhibited good specificity and sensitivity for the majority of established species of the genus Burkholderia. DGGE analysis of the PCR products obtained showed that there were sufficient differences in migration behavior to distinguish the majority of the 14 Burkholderia species tested. Sequence analysis of amplicons generated with soil DNA exclusively revealed sequences affiliated with sequence of Burkholderia species, demonstrating that the PCR-DGGE method is suitable for studying the diversity of this genus in natural settings. A PCR-DGGE analysis of the Burkholderia communities en two grassland plots revealed differences in diversity mainly between bulk and rhizosphere soil samples; the communities in the latter samples produced more complex patterns. |
Thesaurus Nal: |
Burkholderia. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01923naa a2200157 a 4500 001 1625774 005 2004-05-14 008 2002 bl --- 0-- u #d 100 1 $aSALLES, J. F. 245 $aMolecular method to assess the diversity of Burkholderia species in environmental. 260 $c2002 520 $aIn spite of the importance of many members of the genus Burkholderia in the soil microbial community, no direct method to assess the diversity of this genus has been developed so far. The aim of this work was the development of soil DNA-based PCR-denaturing gradient gel electrophoresis (DGGE), a powerful tool for studying the diversity of microbial communities, for detection and analysis of the Burkholderia diversity in soil samples. Primers specific for the genus Burkholderia were developed based on the 16S rDNA gene sequence and were evaluated in PCRs performed with genomic DNAs from Burkholderia and non-Burkholderia species as the templates. The primer system used exhibited good specificity and sensitivity for the majority of established species of the genus Burkholderia. DGGE analysis of the PCR products obtained showed that there were sufficient differences in migration behavior to distinguish the majority of the 14 Burkholderia species tested. Sequence analysis of amplicons generated with soil DNA exclusively revealed sequences affiliated with sequence of Burkholderia species, demonstrating that the PCR-DGGE method is suitable for studying the diversity of this genus in natural settings. A PCR-DGGE analysis of the Burkholderia communities en two grassland plots revealed differences in diversity mainly between bulk and rhizosphere soil samples; the communities in the latter samples produced more complex patterns. 650 $aBurkholderia 700 1 $aSOUZA, F. A. de 700 1 $aVAN ELSAS, J. D. 773 $tApplied and Environmental Microbiology, Washington$gv. 68, n.4, p. 1595-1603, 2002
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
18/07/2007 |
Data da última atualização: |
28/06/2019 |
Tipo da produção científica: |
Folder/Folheto/Cartilha |
Autoria: |
DIAS, R. de C. S.; QUEIROZ, M. A. de; COSTA, N. D.; SOUZA, F. de F.; ALMEIDA, M. C. B. de; ARAUJO, H. M.; LIBERALINO FILHO, J.; PEREIRA, A. B.; BAHIA, J.; LIMA, R. N. dos S.; ANJOS, J. B. dos; PEREIRA, F. A.; ALVES, D. da C.; ARAÚJO, J. P. de. |
Afiliação: |
RITA DE CASSIA SOUZA DIAS, CPATSA; MANOEL ABÍLIO DE QUEIROZ, CPATSA; NIVALDO DUARTE COSTA, CPATSA; FLAVIO DE FRANCA SOUZA, CPATSA; MANOEL CICERO BARBOSA DE ALMEIDA, CPATSA; HELIO MACEDO ARAUJO, CPATSA; JOSE LIBERALINO FILHO, CPATSA; ANTONIO B. PEREIRA, CPATSA; JUSTINO BAHIA, CPATSA; RAIMUNDO N. DOS S. LIMA, CPATSA; JOSE BARBOSA DOS ANJOS, CPATSA; FRANCISCO ALVES PEREIRA, CPATSA; DINIZ DA CONCEICAO ALVES, CPATSA; JOSÉ PIRES DE ARAÚJO, CPATSA. |
Título: |
BRS Opara: melancia resistente ao oídio. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Petrolina: Embrapa Semi-Árido, 2007. |
Páginas: |
Np. |
Descrição Física: |
1 Folder. |
Idioma: |
Português |
Conteúdo: |
Sem doença e produtiva; Características; Recomendações técnica. |
Palavras-Chave: |
BRS Opara; Watermelon. |
Thesagro: |
Doença; Melancia; Oídio; Resistência; Variedade. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/175869/1/35732.pdf
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Marc: |
LEADER 00984nam a2200361 a 4500 001 1158637 005 2019-06-28 008 2007 bl uuuu u0uu1 u #d 100 1 $aDIAS, R. de C. S. 245 $aBRS Opara$bmelancia resistente ao oídio. 260 $aPetrolina: Embrapa Semi-Árido$c2007 300 $aNp.$c1 Folder. 520 $aSem doença e produtiva; Características; Recomendações técnica. 650 $aDoença 650 $aMelancia 650 $aOídio 650 $aResistência 650 $aVariedade 653 $aBRS Opara 653 $aWatermelon 700 1 $aQUEIROZ, M. A. de 700 1 $aCOSTA, N. D. 700 1 $aSOUZA, F. de F. 700 1 $aALMEIDA, M. C. B. de 700 1 $aARAUJO, H. M. 700 1 $aLIBERALINO FILHO, J. 700 1 $aPEREIRA, A. B. 700 1 $aBAHIA, J. 700 1 $aLIMA, R. N. dos S. 700 1 $aANJOS, J. B. dos 700 1 $aPEREIRA, F. A. 700 1 $aALVES, D. da C. 700 1 $aARAÚJO, J. P. de
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Embrapa Semiárido (CPATSA) |
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