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| Registros recuperados : 174 | |
| 82. |  | BAYARDO, R.; SOUZA, F. A. de; MEDICI, L.; ALVES, B. J. R.; BODDEY, R. M.; URQUIAGA, S. Assimilation of ammonium, and response to inoculation with the arbuscular mycorrhizal fungus of different maize genotypes grown in an acid tropical soil. In: INTERNATIONAL SYMPOSIUM ON PLANT-SOIL INTERACTIONS AT LOW pH, 4., 1996, Belo Horizonte. Abstracts/Programs. Sete Lagoas: EMBRAPA-CNPMS, 1996. p.115.| Biblioteca(s): Embrapa Cerrados. |
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| 83. | | MARTINS, A. P. B.; PEREIRA, J. P.; CORRÊA, P. A.; MELO, M. C. C. de; SOUZA, F. A. de. Inoculação de Microrganismos Eficientes (EM) e Fungos Micorrízicos Arbusculares (FMA) em alface: um trabalho de campo conduzido na horta comunitária (HC) Vapabuçu, município de Sete Lagoas- MG. In: CONGRESSO LATINO-AMERICANO DE AGROECOLOGIA, 6.; CONGRESSO BRASILEIRO DE AGROECOLOGIA, 10.; SEMINÁRIO DE AGROECOLOGIA DO DISTRITO FEDERAL E ENTORNO, 5., 2017, Brasília, DF. Agroecologia na transformação dos sistemas agroalimentares na América Latina: memórias, saberes e caminhos para o bem viver: anais. Brasília, DF: Associação Brasileira de Agroecologia, 2017.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 86. | | GOMES, E. A.; SOARES, F. A. de O.; LANA, U. G. de P.; SOUZA, F. A. de. Caracterização molecular de fungos micorrízicos arbusculares associados a genótipos de milho contrastantes para eficiência no uso de fósforo. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 13.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 11.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 8., 2010, Guarapari. Fontes de nutrientes e produção agrícola: modelando o futuro: anais. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2010. FertBio 2010.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 88. | | PARANHOS, B. A. G.; SOUZA, F. A. de; PACHECO, M. G.; VIRGÍNIO, J. F.; BRAVO, I. S. J. Moscas-das-frutas, suas plantas hospedeiras e parasitoides no estado de Pernambuco. In: ZUCCHI, R. A.; MALAVASI, A.; ADAIME, R.; NAIVA, D. E. Moscas-das-frutas no Brasil: conhecimento básico e aplicado. Piracicaba: Fealq, 2023. v. 2, cap. 44, p. 249-257.| Biblioteca(s): Embrapa Semiárido. |
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| 90. | | SILVA, I. R. da; SOUZA, F. A. de; SILVA, D. K. A. da; OEHL, F.; MAIA, L. C. Patterns of arbuscular mycorrhizal fungal distribution on mainland and island sandy coastal plain ecosystems in Brazil. Microbial Ecology, New York, v. 74, n. 3, p. 654-669, 2017. Publicado online em 11 abr. 2017.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 95. | | GARCIA, I. F. F.; ALMEIDA, A. K. de; COSTA, T. I. R.; LEOPOLDINO JÚNIOR, I.; RIBEIRO, J. do S.; SOUZA, F. A. de. Carcass characteristics and cuts of Santa Inês lambs fed different roughage proportions and fat source. Revista Brasileira de Zootecnia, Viçosa, MG, v. 39, n. 6, p. 1322-1327, 2010.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 96. | | SOUZA, I. R. P. de; GIOLITTI, F.; CARNEIRO, N. P.; LENARDON, S. L.; OLIVEIRA, E. de; GOMES, E. A.; NODA, R. W.; SOUZA, F. A. de. Sequence diversity in coat protein of SCMV infecting maize and sorghum in Brazil. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 11, n. 2, p. 120-136, 2012.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 97. | | GOMES, E. A.; OLIVEIRA-PAIVA, C. A.; LANA, U. G. de P.; NODA, R. W.; MARRIEL, I. E.; SOUZA, F. A. de. Influência do pH do solo na comunidade de fungos micorrízicos arbusculares associada a raízes de milho contrastantes para eficiência no uso de fósforo. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26., 2011, Foz do Iguaçu. Resumos. Foz do Iguaçu: Sociedade Brasileira de Microbiologia, 2011.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 98. | | SOUZA, F. A. de; STURMER, S. L.; MOREIRA, F. M.; SIQUEIRA, J. O.; CARVALHO, F. de; LEAL, P. L.; PEREIRA, C. D.; GOMES, E. A.; MARRIEL, I. E. Identificação molecular de Glomeromycetes no Brasil: DNA Barcode. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26., 2011, Foz do Iguaçu. Resumos. Foz do Iguaçu: Sociedade Brasileira de Microbiologia, 2011.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 99. | | CARNEIRO, A. A.; MOREIRA, G. A. dos S.; MEDEIROS, K. M. R.; OLIVEIRA, R. P. de; FERREIRA, C. F.; SOUZA, F. A. de. Indução de culturas de raízes por diferentes linhagens de Agrobacterium rhizogenes em plântulas de Cichorium intybus, Daucus carota, Macroptilium atropurpureum e Plantago sp. Sete Lagoas: Embrapa Milho e Sorgo, 2019. 26 p. (Embrapa Milho e Sorgo. Boletim de Pesquisa e Desenvolvimento, 197).| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 100. | | MARINS, M. S.; OLIVEIRA, R. S. de; ABREU, C. S. de; OLIVEIRA-PAIVA, C. A.; SOUZA, F. A. de; MARRIEL, I. E.; MOREIRA, F. A.; LAGO, R. M. Atividade de micro-organismos solubilizadores de fosfato de ferro em resíduo industrial. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: [anais]. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2016. p. 1000. FertBio 2016.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 174 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
05/07/2023 |
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Data da última atualização: |
10/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
HIMMEN, F. D. A.; SOUZA, F. A. de; SILVA-CARDOSO, I. M. de A.; SOUZA, A. L. X. de; PEREIRA, J. E. S. |
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Afiliação: |
FERNANDA DUARTE ARAÚJO HIMMEN, UNIVERSIDADE DE BRASILIA; FRANCISCO ADRIANO DE SOUZA, CNPMS; INAÊ MARIÊ DE ARAÚJO SILVA-CARDOSO; ANDRE LUIS XAVIER DE SOUZA, Cenargen; JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen. |
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Título: |
Micropropagation, estimation of DNA methylation during multiplication cycles and mycorrhization of seed-derived Dendrocalamus asper (Schultes f.) Backer ex Heyne. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v. 155, p. 41-56, 2023. |
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DOI: |
https://doi.org/10.1007/s11240-023-02547-x |
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Idioma: |
Inglês |
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Conteúdo: |
Considering the economic significance of Dendrocalamus asper, a woody bamboo species, the objective of this study was to establish an in vitro micropropagation and mycorrhization protocol, and to analyze the DNA methylation dynamics during the multiplication stage of plants of this species. For shoot proliferation, meta-topolin (mT), 6-benzylaminopurine (BAP) and kinetin (Kin) were evaluated at different concentrations (0, 3.3, 6.6 and 13.3 µM) during three monthly subcultures. For in vitro mycorrhization, Rhizoglomus clarum inoculated in liquid medium was used. Murashige and Skoog (MS) and Strullu and Romand (MSR) culture media were tested with different modifications, such as reduced phosphorus, sucrose, and salts. The evidence of root colonization (spores, hyphae, or vesicles) was assessed. For micropropagation, it was verified that mT was as efficient as BAP for the in vitro multiplication of D. asper shoots. There was also a substantial reduction in the number of shoots in the third subculture, which coincided with a significant decrease in DNA methylation evaluated via ELISA, unprecedented analysis carried out during bamboo micropropagation. As for mycorrhization, colonization was observed only in plants grown on MSR (16% of the plants) and MS/2 medium with 25% of the total amount of sucrose (25% of the plants). These results will support the development of efficient in vitro mycorrhization protocols for D. asper and, consequently, the optimization of the quality of the micropropagated plantlets. MenosConsidering the economic significance of Dendrocalamus asper, a woody bamboo species, the objective of this study was to establish an in vitro micropropagation and mycorrhization protocol, and to analyze the DNA methylation dynamics during the multiplication stage of plants of this species. For shoot proliferation, meta-topolin (mT), 6-benzylaminopurine (BAP) and kinetin (Kin) were evaluated at different concentrations (0, 3.3, 6.6 and 13.3 µM) during three monthly subcultures. For in vitro mycorrhization, Rhizoglomus clarum inoculated in liquid medium was used. Murashige and Skoog (MS) and Strullu and Romand (MSR) culture media were tested with different modifications, such as reduced phosphorus, sucrose, and salts. The evidence of root colonization (spores, hyphae, or vesicles) was assessed. For micropropagation, it was verified that mT was as efficient as BAP for the in vitro multiplication of D. asper shoots. There was also a substantial reduction in the number of shoots in the third subculture, which coincided with a significant decrease in DNA methylation evaluated via ELISA, unprecedented analysis carried out during bamboo micropropagation. As for mycorrhization, colonization was observed only in plants grown on MSR (16% of the plants) and MS/2 medium with 25% of the total amount of sucrose (25% of the plants). These results will support the development of efficient in vitro mycorrhization protocols for D. asper and, consequently, the optimization of the quality of th... Mostrar Tudo |
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Palavras-Chave: |
Arbuscular mycorrhizal fungi; Bambusoideae; Epigenética; Fungo micorrízico arbuscular; In vitro propagation; Propagação in vitro. |
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Thesagro: |
Bambu; DNA; Fungo; Micorriza; Semente. |
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Thesaurus NAL: |
Bamboos; Epigenetics. |
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Categoria do assunto: |
--
G Melhoramento Genético |
|
Marc: |
LEADER 02599naa a2200337 a 4500
001 2154816
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008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11240-023-02547-x$2DOI
100 1 $aHIMMEN, F. D. A.
245 $aMicropropagation, estimation of DNA methylation during multiplication cycles and mycorrhization of seed-derived Dendrocalamus asper (Schultes f.) Backer ex Heyne.$h[electronic resource]
260 $c2023
520 $aConsidering the economic significance of Dendrocalamus asper, a woody bamboo species, the objective of this study was to establish an in vitro micropropagation and mycorrhization protocol, and to analyze the DNA methylation dynamics during the multiplication stage of plants of this species. For shoot proliferation, meta-topolin (mT), 6-benzylaminopurine (BAP) and kinetin (Kin) were evaluated at different concentrations (0, 3.3, 6.6 and 13.3 µM) during three monthly subcultures. For in vitro mycorrhization, Rhizoglomus clarum inoculated in liquid medium was used. Murashige and Skoog (MS) and Strullu and Romand (MSR) culture media were tested with different modifications, such as reduced phosphorus, sucrose, and salts. The evidence of root colonization (spores, hyphae, or vesicles) was assessed. For micropropagation, it was verified that mT was as efficient as BAP for the in vitro multiplication of D. asper shoots. There was also a substantial reduction in the number of shoots in the third subculture, which coincided with a significant decrease in DNA methylation evaluated via ELISA, unprecedented analysis carried out during bamboo micropropagation. As for mycorrhization, colonization was observed only in plants grown on MSR (16% of the plants) and MS/2 medium with 25% of the total amount of sucrose (25% of the plants). These results will support the development of efficient in vitro mycorrhization protocols for D. asper and, consequently, the optimization of the quality of the micropropagated plantlets.
650 $aBamboos
650 $aEpigenetics
650 $aBambu
650 $aDNA
650 $aFungo
650 $aMicorriza
650 $aSemente
653 $aArbuscular mycorrhizal fungi
653 $aBambusoideae
653 $aEpigenética
653 $aFungo micorrízico arbuscular
653 $aIn vitro propagation
653 $aPropagação in vitro
700 1 $aSOUZA, F. A. de
700 1 $aSILVA-CARDOSO, I. M. de A.
700 1 $aSOUZA, A. L. X. de
700 1 $aPEREIRA, J. E. S.
773 $tPlant Cell, Tissue and Organ Culture$gv. 155, p. 41-56, 2023.
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