Data/hora: 15/06/2024 02:25:31

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 04/12/2017
Data da última atualização: | 04/12/2017
Autoria: | SILVA, R. L. da; SOUZA, E. H. de; VIEIRA, L. de J.; PELACANI, C. R.; SOUZA, F. V. D.
Afiliação: | RONILZE LEITE DA SILVA, EFS; EVERTON HILO DE SOUZA, CAPES; LÍVIA DE JESUS VIEIRA, CAPES; CLAUDINÉIA REGINA PELACANI, UEFS; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF.
Título: | Cryopreservation of pollen of wild pineapple accessions.
Ano de publicação: | 2017
Fonte/Imprenta: | Scientia Horticulturae, v.219, p.326-334, May 2017.
Idioma: | Inglês
Conteúdo: | Cryopreservation of pollen is an additional way to conserve many species. In particular, it can be used as an alternative strategy to preserve the genetic variation of the genus Ananas. The aim of this study was to cryopreserve pollen from wild pineapple accessions to conserve a gene pool of the genus Ananas, as well as to minimize problems of asynchronous flowering among accessions preserved in a germplasm bank. Accessions of all botanical varieties were used to investigate different protocols for freezing pollen, as well as to determine their viability in vitro/in vivo during the investigation and after freezing in liquid nitrogen. Anthers with pollen of the different accessions were dehydrated and then immersed in liquid nitrogen for periods of 24 h, 60 days and 120 days. After freezing, the viability percentage and pollen tube length were determined. In vivo viability tests were performed through 24 pollinations involving different accessions. The results showed that the cryopreservation of pollen was efficient, attaining in vitro germination rates ranging from 8.24% to 94.25%, with average of 62.67% achieved 120 days after cryopreservation and pollen tube lengths longer than 0.23 mm after this same period. In the in vivo pollinations with cryopreserved grains, the seed formation rate was 70.83% for the different combinations. It was possible to cryopreserve pollen from all the pineapple accessions investigated after dehydration for 6 h on silica gel, with significant in vitro germination percentages and production of viable seeds.
Thesagro: | Abacaxi.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (32079-1) - UPC - AP - DD - Publicação digital -  -  - DIS