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Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
30/11/2015 |
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Data da última atualização: |
25/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SILVA, K. J. D. e; SOUZA, E. A.; FREIRE, C. N. S.; ISHIKAWA, F. H. |
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Afiliação: |
KAESEL JACKSON DAMASCENO E SILVA, CPAMN; E.A. Souza, Universidade Federal de Lavras, Laboratório de Resistência de Plantas a Doenças e Genética Molecular, Departamento de Biologia, Lavras, MG.; C.N.S. Freire, Universidade Federal de Lavras, Laboratório de Resistência de Plantas a Doenças e Genética Molecular, Departamento de Biologia, Lavras, MG.; F.H. Ishikawa, Colegiado de Engenharia Agronômica, Universidade Federal do Vale do São Francisco, Petrolina, PE. |
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Título: |
Anastomosis groups and molecular variation in Pseudocercospora griseola. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 14, n. 3, p. 7436-7445, July 2015. |
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ISSN: |
1676-5680 |
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DOI: |
http://dx.doi.org/10.4238/2015.July.3.19 |
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Idioma: |
Inglês |
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Conteúdo: |
The fungus Pseudocercospora griseola is the causal agent of angular leaf spot, a common bean (Phaseolus vulgaris L.) disease. The successful development of angular leaf spot-resistant cultivars depends on understanding the levels of variability in pathogen populations. P. griseola shows great pathogenic and genetic variation using inoculation on differential bean cultivars and molecular markers. Nevertheless, how this variability develops is not clearly understood. Parasexuality has been presented as a mechanism used by asexual fungi to increase variation. Hyphal fusion is the first step for the parasexual cycle, and it can be considered an additional trait for population studies. The aim of this study was to identify hyphal fusion (anastomosis) among P. grisola isolates and to evaluate the variability of isolates using analyses of anastomosis groups and RAPD markers. Hyphal anastomosis was observed in all isolates. Three isolates showed 85.0% compatibility and were compatible with 17 isolates. This is the first report of the occurrence of anastomosis between P. griseola isolates. Sixteen anastomosis groups were observed and only one group was formed by five isolates (Pg-01, Pg-02, Pg-07, Pg-08, and Pg-12). There was a large number of anastomosis groups and absence of clustering among isolates for hyphal fusion, demonstrating the existence of genetic variability for loci involved in the control of this trait. High genotypic variability by RAPD markers was detected, but there was no relationship between genotype and anastomosis group. More studies are necessary to elucidate further the process of asexual recombination in this phytopathogen. MenosThe fungus Pseudocercospora griseola is the causal agent of angular leaf spot, a common bean (Phaseolus vulgaris L.) disease. The successful development of angular leaf spot-resistant cultivars depends on understanding the levels of variability in pathogen populations. P. griseola shows great pathogenic and genetic variation using inoculation on differential bean cultivars and molecular markers. Nevertheless, how this variability develops is not clearly understood. Parasexuality has been presented as a mechanism used by asexual fungi to increase variation. Hyphal fusion is the first step for the parasexual cycle, and it can be considered an additional trait for population studies. The aim of this study was to identify hyphal fusion (anastomosis) among P. grisola isolates and to evaluate the variability of isolates using analyses of anastomosis groups and RAPD markers. Hyphal anastomosis was observed in all isolates. Three isolates showed 85.0% compatibility and were compatible with 17 isolates. This is the first report of the occurrence of anastomosis between P. griseola isolates. Sixteen anastomosis groups were observed and only one group was formed by five isolates (Pg-01, Pg-02, Pg-07, Pg-08, and Pg-12). There was a large number of anastomosis groups and absence of clustering among isolates for hyphal fusion, demonstrating the existence of genetic variability for loci involved in the control of this trait. High genotypic variability by RAPD markers was detected, but there... Mostrar Tudo |
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Palavras-Chave: |
Angular leaf spot; Ciclo parassexual; Common bean; Feijoeiro comum; RAPD markers. |
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Thesagro: |
Mancha angular; Marcador molecular. |
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Thesaurus NAL: |
Parasexual cycle; Phaeoisariopsis griseola. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/134323/1/ArtigoKaeselGMR.pdf
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Marc: |
LEADER 02533naa a2200289 a 4500
001 2030017
005 2022-05-25
008 2015 bl uuuu u00u1 u #d
022 $a1676-5680
024 7 $ahttp://dx.doi.org/10.4238/2015.July.3.19$2DOI
100 1 $aSILVA, K. J. D. e
245 $aAnastomosis groups and molecular variation in Pseudocercospora griseola.
260 $c2015
520 $aThe fungus Pseudocercospora griseola is the causal agent of angular leaf spot, a common bean (Phaseolus vulgaris L.) disease. The successful development of angular leaf spot-resistant cultivars depends on understanding the levels of variability in pathogen populations. P. griseola shows great pathogenic and genetic variation using inoculation on differential bean cultivars and molecular markers. Nevertheless, how this variability develops is not clearly understood. Parasexuality has been presented as a mechanism used by asexual fungi to increase variation. Hyphal fusion is the first step for the parasexual cycle, and it can be considered an additional trait for population studies. The aim of this study was to identify hyphal fusion (anastomosis) among P. grisola isolates and to evaluate the variability of isolates using analyses of anastomosis groups and RAPD markers. Hyphal anastomosis was observed in all isolates. Three isolates showed 85.0% compatibility and were compatible with 17 isolates. This is the first report of the occurrence of anastomosis between P. griseola isolates. Sixteen anastomosis groups were observed and only one group was formed by five isolates (Pg-01, Pg-02, Pg-07, Pg-08, and Pg-12). There was a large number of anastomosis groups and absence of clustering among isolates for hyphal fusion, demonstrating the existence of genetic variability for loci involved in the control of this trait. High genotypic variability by RAPD markers was detected, but there was no relationship between genotype and anastomosis group. More studies are necessary to elucidate further the process of asexual recombination in this phytopathogen.
650 $aParasexual cycle
650 $aPhaeoisariopsis griseola
650 $aMancha angular
650 $aMarcador molecular
653 $aAngular leaf spot
653 $aCiclo parassexual
653 $aCommon bean
653 $aFeijoeiro comum
653 $aRAPD markers
700 1 $aSOUZA, E. A.
700 1 $aFREIRE, C. N. S.
700 1 $aISHIKAWA, F. H.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 14, n. 3, p. 7436-7445, July 2015.
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