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| Registros recuperados : 62 | |
| 13. |  | DIAMANTINO, E. P.; SOUZA, A. de M.; SANCHES, N. F. Indices de infestacao do acaro alaranjado Dolichotetranychus floridanus (Acari, tenuipalpidae) em abacaxizeiro sob regime de irrigacao Salvador, BA: Sociedade Entomologica do Brasil/EMBRAPA-CNPMF, 1997 p.42 In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 16, 1997, Salvador, BA.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 17. | | SOUZA, A. de M.; DIAMANTINO, E. P.; SANCHES, N. F. Incidencia e distribuicao do acaro alaranjado Dolichotetranychus floridanus (Acari, tenuipalpidae) em abacaxizeiro. Salvador, BA: Sociedade Entomologica do Brasil/EMBRAPA-CNPMF, 1997 p.43 In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 16, 1997, Salvador,BA| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 62 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
30/11/2021 |
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Data da última atualização: |
30/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MURARO, L. S.; NOGUEIRA, M. F.; BORGES, A. M. C. M.; SOUZA, A. de O.; VIEIRA, T. S. W. J.; AGUIAR, D. M. de. |
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Afiliação: |
LÍVIA SAAB MURARO, University of Cuiaba; MARCIA FURLAN NOGUEIRA T DE LIMA, CPAP; ALICE MAMEDE COSTA MARQUES BORGES, Federal University of Mato Grosso; ANELIZA DE OLIVEIRA SOUZA, University of Cuiaba; THALLITHA SAMIH WISCHRAL JAYME VIEIRA, Federal University of Parana; DANIEL MOURA DE AGUIAR, Federal University of Mato Grosso. |
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Título: |
Detection of Ehrlichia sp. in Amblyomma sculptum parasitizing horses from Brazilian Pantanal wetland. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Ticks and Tick-borne Diseases, v. 12, 101658, 2021. |
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Idioma: |
Português |
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Conteúdo: |
Bacteria of the genus Ehrlichia are transmitted by ticks and also are an important cause of infection in wild and domestic mammals. Infection with Ehrlichia spp. has been reported in horses, especially in the USA, Nicaragua and Brazil. In this study, we report the parasitism by Amblyomma sculptum, Rhipicephalus microplus and Dermacentor nitens ticks in horses from a ranch located in south Pantanal wetland. Molecular and serological analyzes to determine infection by Ehrlichia spp. in horses and their respective ticks were carried out. A total of 12 horses were submitted to blood collection to investigate antibodies by indirect immunofluorescence assay (IFA) using Ehrlichia canis crude antigens and to be tested by polymerase chain reaction (PCR) in order to amplify fragments of the 16S rRNA, dsb, groEL and sodB gene of Ehrlichia spp. A total of 164 tick specimens were removed from horses, stored in isopropanol and later identified as D. nitens, A. sculptum and R. microplus. DNA from ticks were extracted and subjected to the same PCR assays to detect Ehrlichia spp. Anti-Ehrlichia spp. antibodies were detected in five/12 (41.7 %) horses by IFA, with antibody titers ranging from 40 to 160. All horse DNA samples were negative for the 16S rRNA, dsb, groEL and sodB of Ehrlichia spp. One A. sculptum female was positive to all target genes of Ehrlichia. This tick was parasitizing an Ehrlichia-seropositive horse with antibody titer of 80. Nucleotide sequences of 16S rRNA, dsb, groEL and sodB genes showed close relationship with different strains of Ehrlichia detected in wild mammals, Amblyomma ticks and horses from Brazil and Argentina. Detection of antiEhrlichia sp. antibodies suggests that horses have been exposed to an ehrlichial agent in the Pantanal. Future studies on Ehrlichia infection should be carried out to better elucidate and to bring new information about equine ehrlichiosis, since these animals are important hosts of ticks in the Brazilian Pantanal wetlands. MenosBacteria of the genus Ehrlichia are transmitted by ticks and also are an important cause of infection in wild and domestic mammals. Infection with Ehrlichia spp. has been reported in horses, especially in the USA, Nicaragua and Brazil. In this study, we report the parasitism by Amblyomma sculptum, Rhipicephalus microplus and Dermacentor nitens ticks in horses from a ranch located in south Pantanal wetland. Molecular and serological analyzes to determine infection by Ehrlichia spp. in horses and their respective ticks were carried out. A total of 12 horses were submitted to blood collection to investigate antibodies by indirect immunofluorescence assay (IFA) using Ehrlichia canis crude antigens and to be tested by polymerase chain reaction (PCR) in order to amplify fragments of the 16S rRNA, dsb, groEL and sodB gene of Ehrlichia spp. A total of 164 tick specimens were removed from horses, stored in isopropanol and later identified as D. nitens, A. sculptum and R. microplus. DNA from ticks were extracted and subjected to the same PCR assays to detect Ehrlichia spp. Anti-Ehrlichia spp. antibodies were detected in five/12 (41.7 %) horses by IFA, with antibody titers ranging from 40 to 160. All horse DNA samples were negative for the 16S rRNA, dsb, groEL and sodB of Ehrlichia spp. One A. sculptum female was positive to all target genes of Ehrlichia. This tick was parasitizing an Ehrlichia-seropositive horse with antibody titer of 80. Nucleotide sequences of 16S rRNA, dsb, groEL a... Mostrar Tudo |
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Thesagro: |
Carrapato; Cavalo; Infecção. |
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Thesaurus NAL: |
Horse diseases; Infection; Tick control. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02726naa a2200253 a 4500
001 2136832
005 2021-11-30
008 2021 bl uuuu u00u1 u #d
100 1 $aMURARO, L. S.
245 $aDetection of Ehrlichia sp. in Amblyomma sculptum parasitizing horses from Brazilian Pantanal wetland.$h[electronic resource]
260 $c2021
520 $aBacteria of the genus Ehrlichia are transmitted by ticks and also are an important cause of infection in wild and domestic mammals. Infection with Ehrlichia spp. has been reported in horses, especially in the USA, Nicaragua and Brazil. In this study, we report the parasitism by Amblyomma sculptum, Rhipicephalus microplus and Dermacentor nitens ticks in horses from a ranch located in south Pantanal wetland. Molecular and serological analyzes to determine infection by Ehrlichia spp. in horses and their respective ticks were carried out. A total of 12 horses were submitted to blood collection to investigate antibodies by indirect immunofluorescence assay (IFA) using Ehrlichia canis crude antigens and to be tested by polymerase chain reaction (PCR) in order to amplify fragments of the 16S rRNA, dsb, groEL and sodB gene of Ehrlichia spp. A total of 164 tick specimens were removed from horses, stored in isopropanol and later identified as D. nitens, A. sculptum and R. microplus. DNA from ticks were extracted and subjected to the same PCR assays to detect Ehrlichia spp. Anti-Ehrlichia spp. antibodies were detected in five/12 (41.7 %) horses by IFA, with antibody titers ranging from 40 to 160. All horse DNA samples were negative for the 16S rRNA, dsb, groEL and sodB of Ehrlichia spp. One A. sculptum female was positive to all target genes of Ehrlichia. This tick was parasitizing an Ehrlichia-seropositive horse with antibody titer of 80. Nucleotide sequences of 16S rRNA, dsb, groEL and sodB genes showed close relationship with different strains of Ehrlichia detected in wild mammals, Amblyomma ticks and horses from Brazil and Argentina. Detection of antiEhrlichia sp. antibodies suggests that horses have been exposed to an ehrlichial agent in the Pantanal. Future studies on Ehrlichia infection should be carried out to better elucidate and to bring new information about equine ehrlichiosis, since these animals are important hosts of ticks in the Brazilian Pantanal wetlands.
650 $aHorse diseases
650 $aInfection
650 $aTick control
650 $aCarrapato
650 $aCavalo
650 $aInfecção
700 1 $aNOGUEIRA, M. F.
700 1 $aBORGES, A. M. C. M.
700 1 $aSOUZA, A. de O.
700 1 $aVIEIRA, T. S. W. J.
700 1 $aAGUIAR, D. M. de
773 $tTicks and Tick-borne Diseases$gv. 12, 101658, 2021.
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