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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
20/12/2019 |
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Data da última atualização: |
20/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
IARK, D.; BUZZO, A. J. dos R.; GARCIA, J. A. A.; CORREA, V. G.; HELM, C. V.; CORRÊA, R. C. G.; PERALTA, R. A.; MOREIRA, R. de F. P. M.; BRACHT, A.; PERALTA, R. M. |
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Afiliação: |
Daiane Iark, UEM; Ana Júlia dos Reis Buzzo, UEM; Jéssica Amanda Andrade Garcia, UEM; Vanesa Gesser Correa, UEM; CRISTIANE VIEIRA HELM, CNPF; Rúbia Carvalho Gomes Corrêa, UEM; Rosely A. Peralta, UFSC; Regina de Fátima Peralta Muniz Moreira, UFSC; Adelar Bracht, UEM; Rosane Marina Peralta, UEM. |
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Título: |
Enzymatic degradation and detoxification of azo dye Congo red by a new laccase from Oudemansiella canarii. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Bioresource Technology, 289, article 121655, Oct. 2019. 7 p. |
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DOI: |
10.1016/j.biortech.2019.121655 |
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Idioma: |
Inglês |
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Conteúdo: |
A single laccase with molecular weight of 41 kDa was produced by the white-rot fungus Oudemansiella canarii cultured on solid state fermentation using a mixture of sugarcane bagasse-wheat bran as substrate. The enzyme (5 U) was able to decolourize 80% of 50 mg/L Congo red within 24 h at 30 °C and pH 5.5. The relationship between the decolorization rate and dye concentration obeyed Michaelis?Menten kinetics, with KM and Vmax values of 46.180 ± 6.245 µM and 1.840 ± 0.101 µmol/min, respectively. Fourier transform infrared spectroscopy (FTIR) and mass spectrometry allowed to conclude that the laccase acts not only on the dye chromophore group, but also that it cleaves different covalent bonds, causing an effective fragmentation of the molecule. The action of the laccase caused a significant reduction in toxicity, as indicated by the Microtox test. In conclusion, O. canarii laccase could be useful in future biological strategies aiming at degrading azo dyes. |
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Palavras-Chave: |
Biorremediação; Dye degradation; Lacase. |
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Thesaurus Nal: |
Azo dyes; Laccase; White-rot fungi. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01891naa a2200313 a 4500
001 2117496
005 2019-12-20
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.biortech.2019.121655$2DOI
100 1 $aIARK, D.
245 $aEnzymatic degradation and detoxification of azo dye Congo red by a new laccase from Oudemansiella canarii.$h[electronic resource]
260 $c2019
520 $aA single laccase with molecular weight of 41 kDa was produced by the white-rot fungus Oudemansiella canarii cultured on solid state fermentation using a mixture of sugarcane bagasse-wheat bran as substrate. The enzyme (5 U) was able to decolourize 80% of 50 mg/L Congo red within 24 h at 30 °C and pH 5.5. The relationship between the decolorization rate and dye concentration obeyed Michaelis?Menten kinetics, with KM and Vmax values of 46.180 ± 6.245 µM and 1.840 ± 0.101 µmol/min, respectively. Fourier transform infrared spectroscopy (FTIR) and mass spectrometry allowed to conclude that the laccase acts not only on the dye chromophore group, but also that it cleaves different covalent bonds, causing an effective fragmentation of the molecule. The action of the laccase caused a significant reduction in toxicity, as indicated by the Microtox test. In conclusion, O. canarii laccase could be useful in future biological strategies aiming at degrading azo dyes.
650 $aAzo dyes
650 $aLaccase
650 $aWhite-rot fungi
653 $aBiorremediação
653 $aDye degradation
653 $aLacase
700 1 $aBUZZO, A. J. dos R.
700 1 $aGARCIA, J. A. A.
700 1 $aCORREA, V. G.
700 1 $aHELM, C. V.
700 1 $aCORRÊA, R. C. G.
700 1 $aPERALTA, R. A.
700 1 $aMOREIRA, R. de F. P. M.
700 1 $aBRACHT, A.
700 1 $aPERALTA, R. M.
773 $tBioresource Technology, 289, article 121655, Oct. 2019. 7 p.
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Registro original: |
Embrapa Florestas (CNPF) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
03/02/2012 |
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Data da última atualização: |
06/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SANTANA, J. R. F. de; PAIVA, R.; SOUZA, A. V. de; OLIVEIRA, L. M. de. |
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Afiliação: |
JOSÉ RANIERE FERREIRA DE SANTANA; RENATO PAIVA; ANA VALERIA DE SOUZA, CPATSA; LENALDO MUNIZ DE OLIVEIRA. |
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Título: |
Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Ciência e Agrotecnologia, v. 35, n. 5, p. 916-923, set./out. 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
The present work evaluated the effects of different types of culture flask seals and varying concentrations of sucrose and activated charcoal on the in vitro induction and growth of buds of Annona glabra L.; an edible fruit-producing species popularly known as “araticum”. Nodal segments obtained from A. glabra plants maintained in green houses were surface sterilized and inoculated into a WPM culture medium solidified with 7 g L-1 of agar and supplemented with sucrose (0.00; 29.21; 58.63 and 116.84 mM), activated charcoal (0.0 and 2.0 g L-1), and 250 mg L-1 benomyl. In addition to the varying concentrations of sucrose and activated charcoal, we evaluated the efficiency of two types of test tube seals: PVC film, and cotton plugs. All possible combinations of caps and nutrient media were tested with 4 repetitions with 5 tubes each, evaluating the number of buds, the percentage of explant responses, the number of expanded leaves per bud, the length of the largest leaves, leaf abscission, and the length and dry weight of the buds. The type of seal influenced organogenesis in nodal segments of A. glabra, and no bud induction was observed in the absence of sucrose. The largest number of expanded leaves were obtained when 58.42 mM of sucrose was used in tubes sealed with cotton plugs, and leaf abscission was halved in the presence of activated charcoal. The greatest bud length and dry weight were obtained in tubes sealed with cotton plugs and in the presence of activated charcoa |
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Palavras-Chave: |
Abscisão foliar; Cultivo in vitro; Tampão de algodão. |
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Thesagro: |
Annona glabra; Araticum; Fruta Tropical. |
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Thesaurus NAL: |
Fruits; Leaf abscission. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53397/1/Ana-Valeria-2011.pdf
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Marc: |
LEADER 02308naa a2200253 a 4500
001 1914333
005 2023-06-06
008 2011 bl uuuu u00u1 u #d
100 1 $aSANTANA, J. R. F. de
245 $aEffect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L.$h[electronic resource]
260 $c2011
520 $aThe present work evaluated the effects of different types of culture flask seals and varying concentrations of sucrose and activated charcoal on the in vitro induction and growth of buds of Annona glabra L.; an edible fruit-producing species popularly known as “araticum”. Nodal segments obtained from A. glabra plants maintained in green houses were surface sterilized and inoculated into a WPM culture medium solidified with 7 g L-1 of agar and supplemented with sucrose (0.00; 29.21; 58.63 and 116.84 mM), activated charcoal (0.0 and 2.0 g L-1), and 250 mg L-1 benomyl. In addition to the varying concentrations of sucrose and activated charcoal, we evaluated the efficiency of two types of test tube seals: PVC film, and cotton plugs. All possible combinations of caps and nutrient media were tested with 4 repetitions with 5 tubes each, evaluating the number of buds, the percentage of explant responses, the number of expanded leaves per bud, the length of the largest leaves, leaf abscission, and the length and dry weight of the buds. The type of seal influenced organogenesis in nodal segments of A. glabra, and no bud induction was observed in the absence of sucrose. The largest number of expanded leaves were obtained when 58.42 mM of sucrose was used in tubes sealed with cotton plugs, and leaf abscission was halved in the presence of activated charcoal. The greatest bud length and dry weight were obtained in tubes sealed with cotton plugs and in the presence of activated charcoa
650 $aFruits
650 $aLeaf abscission
650 $aAnnona glabra
650 $aAraticum
650 $aFruta Tropical
653 $aAbscisão foliar
653 $aCultivo in vitro
653 $aTampão de algodão
700 1 $aPAIVA, R.
700 1 $aSOUZA, A. V. de
700 1 $aOLIVEIRA, L. M. de
773 $tCiência e Agrotecnologia$gv. 35, n. 5, p. 916-923, set./out. 2011.
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