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| Registros recuperados : 12 | |
| 4. |  | FRUGERI, G. C.; NOGUEIRA, G. F.; SOUZA, A. L. X. de; PEREIRA, J. E. S. Designing ex situ conservation strategies for Butia capitata [Mart. (Becc.) Arecaceae], a threatened palm tree from Brazilian Savannah Biome, through zygotic embryo cryopreservation. International Journal Plant Biollogy, v. 14, n. 3, p. 612-624, 2023. Na publicação: Jonny Everson Scherwinski-Pereira.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | HIMMEN, F. D. A.; SOUZA, F. A. de; SILVA-CARDOSO, I. M. de A.; SOUZA, A. L. X. de; PEREIRA, J. E. S. Micropropagation, estimation of DNA methylation during multiplication cycles and mycorrhization of seed-derived Dendrocalamus asper (Schultes f.) Backer ex Heyne. Plant Cell, Tissue and Organ Culture, v. 155, p. 41-56, 2023.| Biblioteca(s): Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | SILVA-CARDOSO, I. M. de A.; MEIRA, R. O.; NEVES, J. dos S.; SOUZA, A. L. X. de; CUNHA, R. N. V. da; LOPES, R.; SCHERWINSKI-PEREIRA, J. E. Dinâmica da metilação durante a indução de calos em explantes foliares de Elaeis oleifera X E. Ghineensis durante a embriogênese somática. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 6., 2020. Anais... Fortaleza: Sociedade Brasileira de Recursos Genéticos, 2020. O evento foi realizado de 09 a 12 de novembro de 2020 em formato totalmente digital.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | CARDOSO, I. M. de A. S.; MEIRA, R. O.; NEVES, J. dos S.; SOUZA, A. L. X. de; CUNHA, R. N. V. da; LOPES, R.; PEREIRA, J. E. S. Dinâmica da metilação durante a indução de calos em explantes foliares de Elaeis oleifera x E. guineensis durante a embriogênese somática. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 6., 2020. Recursos genéticos e bioeconomia: inovação para um futuro sustentável: anais. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2020. Trabalho 202.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 11. | | GOMES, H. T.; MACHADO, L. G.; BARTOS, P. M. C.; BALZON, T. A.; COSTA, F. H. da S.; AZEVEDO, V. C. R.; CARDOSO, I. M. de A. S.; SOUZA, A. L. X. de; INGLIS, P. W.; PEREIRA, J. E. S. Somatic embryogenesis in oil palm from immature leaves with emphasis on leaf position, sequential callus re-collection, use of temporary immersion system, and assessment of genetic and epigenetic fidelity of the resulting clones. Plant Cell, Tissue and Organ Culture, v. 156, 32, 2024 Na publicação: Jonny Everson Scherwinski-Pereira.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | MEDEIROS, M. O.; SILVA-CARDOSO, I. M. de A.; COSTA, F. H. da S.; QUEIROZ, P. R. M.; ECKSTEIN, B.; SOUZA, A. L. X. de; PEREIRA, J. E. S. Unveiling innovations for enhancing in vitro propagation of Vanilla phaeantha Rchb.f. through the use of double-phase technique and temporary immersion bioreactor systems. Industrial Crops and Products, v. 216, 118726, 2024. Na publicação: Jonny Everson Scherwinski-Pereira.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 12 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
05/07/2023 |
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Data da última atualização: |
10/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
HIMMEN, F. D. A.; SOUZA, F. A. de; SILVA-CARDOSO, I. M. de A.; SOUZA, A. L. X. de; PEREIRA, J. E. S. |
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Afiliação: |
FERNANDA DUARTE ARAÚJO HIMMEN, UNIVERSIDADE DE BRASILIA; FRANCISCO ADRIANO DE SOUZA, CNPMS; INAÊ MARIÊ DE ARAÚJO SILVA-CARDOSO; ANDRE LUIS XAVIER DE SOUZA, Cenargen; JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen. |
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Título: |
Micropropagation, estimation of DNA methylation during multiplication cycles and mycorrhization of seed-derived Dendrocalamus asper (Schultes f.) Backer ex Heyne. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v. 155, p. 41-56, 2023. |
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DOI: |
https://doi.org/10.1007/s11240-023-02547-x |
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Idioma: |
Inglês |
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Conteúdo: |
Considering the economic significance of Dendrocalamus asper, a woody bamboo species, the objective of this study was to establish an in vitro micropropagation and mycorrhization protocol, and to analyze the DNA methylation dynamics during the multiplication stage of plants of this species. For shoot proliferation, meta-topolin (mT), 6-benzylaminopurine (BAP) and kinetin (Kin) were evaluated at different concentrations (0, 3.3, 6.6 and 13.3 µM) during three monthly subcultures. For in vitro mycorrhization, Rhizoglomus clarum inoculated in liquid medium was used. Murashige and Skoog (MS) and Strullu and Romand (MSR) culture media were tested with different modifications, such as reduced phosphorus, sucrose, and salts. The evidence of root colonization (spores, hyphae, or vesicles) was assessed. For micropropagation, it was verified that mT was as efficient as BAP for the in vitro multiplication of D. asper shoots. There was also a substantial reduction in the number of shoots in the third subculture, which coincided with a significant decrease in DNA methylation evaluated via ELISA, unprecedented analysis carried out during bamboo micropropagation. As for mycorrhization, colonization was observed only in plants grown on MSR (16% of the plants) and MS/2 medium with 25% of the total amount of sucrose (25% of the plants). These results will support the development of efficient in vitro mycorrhization protocols for D. asper and, consequently, the optimization of the quality of the micropropagated plantlets. MenosConsidering the economic significance of Dendrocalamus asper, a woody bamboo species, the objective of this study was to establish an in vitro micropropagation and mycorrhization protocol, and to analyze the DNA methylation dynamics during the multiplication stage of plants of this species. For shoot proliferation, meta-topolin (mT), 6-benzylaminopurine (BAP) and kinetin (Kin) were evaluated at different concentrations (0, 3.3, 6.6 and 13.3 µM) during three monthly subcultures. For in vitro mycorrhization, Rhizoglomus clarum inoculated in liquid medium was used. Murashige and Skoog (MS) and Strullu and Romand (MSR) culture media were tested with different modifications, such as reduced phosphorus, sucrose, and salts. The evidence of root colonization (spores, hyphae, or vesicles) was assessed. For micropropagation, it was verified that mT was as efficient as BAP for the in vitro multiplication of D. asper shoots. There was also a substantial reduction in the number of shoots in the third subculture, which coincided with a significant decrease in DNA methylation evaluated via ELISA, unprecedented analysis carried out during bamboo micropropagation. As for mycorrhization, colonization was observed only in plants grown on MSR (16% of the plants) and MS/2 medium with 25% of the total amount of sucrose (25% of the plants). These results will support the development of efficient in vitro mycorrhization protocols for D. asper and, consequently, the optimization of the quality of th... Mostrar Tudo |
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Palavras-Chave: |
Arbuscular mycorrhizal fungi; Bambusoideae; Epigenética; Fungo micorrízico arbuscular; In vitro propagation; Propagação in vitro. |
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Thesagro: |
Bambu; DNA; Fungo; Micorriza; Semente. |
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Thesaurus NAL: |
Bamboos; Epigenetics. |
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Categoria do assunto: |
--
G Melhoramento Genético |
|
Marc: |
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024 7 $ahttps://doi.org/10.1007/s11240-023-02547-x$2DOI
100 1 $aHIMMEN, F. D. A.
245 $aMicropropagation, estimation of DNA methylation during multiplication cycles and mycorrhization of seed-derived Dendrocalamus asper (Schultes f.) Backer ex Heyne.$h[electronic resource]
260 $c2023
520 $aConsidering the economic significance of Dendrocalamus asper, a woody bamboo species, the objective of this study was to establish an in vitro micropropagation and mycorrhization protocol, and to analyze the DNA methylation dynamics during the multiplication stage of plants of this species. For shoot proliferation, meta-topolin (mT), 6-benzylaminopurine (BAP) and kinetin (Kin) were evaluated at different concentrations (0, 3.3, 6.6 and 13.3 µM) during three monthly subcultures. For in vitro mycorrhization, Rhizoglomus clarum inoculated in liquid medium was used. Murashige and Skoog (MS) and Strullu and Romand (MSR) culture media were tested with different modifications, such as reduced phosphorus, sucrose, and salts. The evidence of root colonization (spores, hyphae, or vesicles) was assessed. For micropropagation, it was verified that mT was as efficient as BAP for the in vitro multiplication of D. asper shoots. There was also a substantial reduction in the number of shoots in the third subculture, which coincided with a significant decrease in DNA methylation evaluated via ELISA, unprecedented analysis carried out during bamboo micropropagation. As for mycorrhization, colonization was observed only in plants grown on MSR (16% of the plants) and MS/2 medium with 25% of the total amount of sucrose (25% of the plants). These results will support the development of efficient in vitro mycorrhization protocols for D. asper and, consequently, the optimization of the quality of the micropropagated plantlets.
650 $aBamboos
650 $aEpigenetics
650 $aBambu
650 $aDNA
650 $aFungo
650 $aMicorriza
650 $aSemente
653 $aArbuscular mycorrhizal fungi
653 $aBambusoideae
653 $aEpigenética
653 $aFungo micorrízico arbuscular
653 $aIn vitro propagation
653 $aPropagação in vitro
700 1 $aSOUZA, F. A. de
700 1 $aSILVA-CARDOSO, I. M. de A.
700 1 $aSOUZA, A. L. X. de
700 1 $aPEREIRA, J. E. S.
773 $tPlant Cell, Tissue and Organ Culture$gv. 155, p. 41-56, 2023.
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