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Registro Completo |
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Biblioteca(s): |
Embrapa Acre. |
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Data corrente: |
05/10/2011 |
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Data da última atualização: |
06/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PERSEGUINI, J. M. C. K.; CHIORATTO, A. F.; ZUCCHI, M. I.; COLOMBO, C. A.; CARBONELL, S. A. M.; MONDEGO, J. M. C.; GAZAFFI, R.; GARCIA, A. A. F.; CAMPOS, T. de; SOUZA, A. P. de; RUBIANO, L. B. |
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Afiliação: |
Juliana Morini Küpper Cardoso Perseguini, IAC; Alisson F ernando Chioratto, IAC; Maria Imaculada Zucchi, IAC; Carlos Augusto Colombo, IAC; Sérgio Augusto Moraes Carbonell, IAC; Jorge Mauricio Costa Mondego, IAC; Rodrigo Gazaffi, ESALQ; Antonio Augusto Franco Garcia, ESALQ; TATIANA DE CAMPOS, CPAF-AC; Anete Pereira de Souza, UNICAMP; Luciana Benchimol Rubiano, IAC. |
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Título: |
Genetic diversity in cultivated carioca common beans based on molecular marker analysis. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 34, n. 1, p. 88-102, 2011. |
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DOI: |
10.1590/S1415-47572011000100017 |
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Idioma: |
Inglês |
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Conteúdo: |
A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger?s modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were polymorphic and 20 AFLP primer combinations produced 635 polymorphic bands. Molecular analysis showed that carioca genotypes were quite diverse. AFLPs revealed greater genetic differentiation and variation within the carioca genotypes (Gst = 98% and Fst = 0.83, respectively) than SSRs and provided better resolution for clustering the carioca genotypes. SSRs and AFLPs were both suitable for assessing the genetic diversity of Brazilian carioca genotypes since the number of markers used in each system provided a low coefficient of variation. However, fingerprint profiles were generated faster with AFLPs, making them a better choice for assessing genetic diversity in the carioca germplasm. |
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Palavras-Chave: |
Feijão carioca; Fitomejoramiento; Frijoles; Marcador microssatélite; Marcadores genéticos; Repeticiones de microsatélite; Variación genética. |
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Thesagro: |
Feijão; Marcador genético; Melhoramento genético vegetal; Phaseolus vulgaris; Polimorfismo genético; Variação genética. |
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Thesaurus Nal: |
Beans; Genetic markers; Genetic polymorphism; Genetic variation; Microsatellite repeats; Plant breeding. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/56462/1/23952.pdf
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Marc: |
LEADER 02912naa a2200481 a 4500
001 1902577
005 2021-07-06
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1590/S1415-47572011000100017$2DOI
100 1 $aPERSEGUINI, J. M. C. K.
245 $aGenetic diversity in cultivated carioca common beans based on molecular marker analysis.$h[electronic resource]
260 $c2011
520 $aA wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger?s modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were polymorphic and 20 AFLP primer combinations produced 635 polymorphic bands. Molecular analysis showed that carioca genotypes were quite diverse. AFLPs revealed greater genetic differentiation and variation within the carioca genotypes (Gst = 98% and Fst = 0.83, respectively) than SSRs and provided better resolution for clustering the carioca genotypes. SSRs and AFLPs were both suitable for assessing the genetic diversity of Brazilian carioca genotypes since the number of markers used in each system provided a low coefficient of variation. However, fingerprint profiles were generated faster with AFLPs, making them a better choice for assessing genetic diversity in the carioca germplasm.
650 $aBeans
650 $aGenetic markers
650 $aGenetic polymorphism
650 $aGenetic variation
650 $aMicrosatellite repeats
650 $aPlant breeding
650 $aFeijão
650 $aMarcador genético
650 $aMelhoramento genético vegetal
650 $aPhaseolus vulgaris
650 $aPolimorfismo genético
650 $aVariação genética
653 $aFeijão carioca
653 $aFitomejoramiento
653 $aFrijoles
653 $aMarcador microssatélite
653 $aMarcadores genéticos
653 $aRepeticiones de microsatélite
653 $aVariación genética
700 1 $aCHIORATTO, A. F.
700 1 $aZUCCHI, M. I.
700 1 $aCOLOMBO, C. A.
700 1 $aCARBONELL, S. A. M.
700 1 $aMONDEGO, J. M. C.
700 1 $aGAZAFFI, R.
700 1 $aGARCIA, A. A. F.
700 1 $aCAMPOS, T. de
700 1 $aSOUZA, A. P. de
700 1 $aRUBIANO, L. B.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 34, n. 1, p. 88-102, 2011.
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Registro original: |
Embrapa Acre (CPAF-AC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
04/08/2015 |
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Data da última atualização: |
24/09/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
C - 0 |
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Autoria: |
SOUZA, A. C. A de; CORTES, M. V. de B.; SILVA, G. B. da; SOUSA, T. P. de; RODRIGUES, F. A.; FILIPPI, M. C. C. |
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Afiliação: |
ALAN CARLOS A. DE SOUZA, UFLA; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; GISELE BARATA DA SILVA, UFRA; THATYANE PEREIRA DE SOUZA, UFG; FABRICIO A. RODRIGUES, UFV; MARTA CRISTINA CORSI DE FILIPPI, CNPAF. |
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Título: |
Enzyme-induced defense response in the suppression of rice leaf blast (Magnaporthe Oryzae) by silicon fertilization and bioagents. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
International Journal of Research Studies in Biosciences, v. 3, n. 5, p. 22-32, May 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
The effects of silicon fertilization and bioagents in suppressing leaf blast were studied in two greenhouse experiments using a susceptible upland rice cultivar, BRS Primavera. In the first experiment, the treatments consisted of four doses of SiCaMg (1,2,4 and 8 tons Si.ha-1) and four bioagent (Burkholderia pyrrocinia (Bp); Pseudomonas fluorescens (Pf); Trichoderma asperellum (Ta) and a mixture of the three bioagents (Bp+Pf+Ta), in addition to untreated controls. The most effective treatment was silicon fertilization at 2tons in combination with the three bioagents, which reduced leaf blast by 96%. A second experiment was conducted to study the defense mechanism involved in disease suppression. The treatments were organized in a randomized block design, with or without Si (0 or 2 tons Si) and bioagents (non-inoculated control, Ta or Bp+Pf+Ta). The combination of silicon fertilization (2 tons SiCaMg.ha-1) with T. asperellum or with a mixture of the three bioagents increased the activities of chitinase (CHI), β-1,3glucanase (GLU), peroxidase (POX) and phenylalanine ammonia-lyase (PAL) as well as the salicylic acid (SA) content in rice plants in the absence of the pathogen Magnaporthe oryzae. The mixture of the three bioagents in plants fertilized with 2 tons Si increased GLU enzyme activity and SA levels at 24 and 48 hours after infection of the plants challenged with M. oryzae. The activation of defense mechanism by combination of silicon fertilization with bioagent mixture could be an effective strategy for rice blast management. MenosThe effects of silicon fertilization and bioagents in suppressing leaf blast were studied in two greenhouse experiments using a susceptible upland rice cultivar, BRS Primavera. In the first experiment, the treatments consisted of four doses of SiCaMg (1,2,4 and 8 tons Si.ha-1) and four bioagent (Burkholderia pyrrocinia (Bp); Pseudomonas fluorescens (Pf); Trichoderma asperellum (Ta) and a mixture of the three bioagents (Bp+Pf+Ta), in addition to untreated controls. The most effective treatment was silicon fertilization at 2tons in combination with the three bioagents, which reduced leaf blast by 96%. A second experiment was conducted to study the defense mechanism involved in disease suppression. The treatments were organized in a randomized block design, with or without Si (0 or 2 tons Si) and bioagents (non-inoculated control, Ta or Bp+Pf+Ta). The combination of silicon fertilization (2 tons SiCaMg.ha-1) with T. asperellum or with a mixture of the three bioagents increased the activities of chitinase (CHI), β-1,3glucanase (GLU), peroxidase (POX) and phenylalanine ammonia-lyase (PAL) as well as the salicylic acid (SA) content in rice plants in the absence of the pathogen Magnaporthe oryzae. The mixture of the three bioagents in plants fertilized with 2 tons Si increased GLU enzyme activity and SA levels at 24 and 48 hours after infection of the plants challenged with M. oryzae. The activation of defense mechanism by combination of silicon fertilization with bioagent mix... Mostrar Tudo |
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Palavras-Chave: |
Bioagente. |
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Thesagro: |
Arroz; Brusone; Doença de planta; Oryza sativa; Silício. |
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Thesaurus NAL: |
Magnaporthe oryzae. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/127449/1/CNPAF2015.pdf
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Marc: |
LEADER 02393naa a2200265 a 4500
001 2021319
005 2015-09-24
008 2015 bl uuuu u00u1 u #d
100 1 $aSOUZA, A. C. A de
245 $aEnzyme-induced defense response in the suppression of rice leaf blast (Magnaporthe Oryzae) by silicon fertilization and bioagents.$h[electronic resource]
260 $c2015
520 $aThe effects of silicon fertilization and bioagents in suppressing leaf blast were studied in two greenhouse experiments using a susceptible upland rice cultivar, BRS Primavera. In the first experiment, the treatments consisted of four doses of SiCaMg (1,2,4 and 8 tons Si.ha-1) and four bioagent (Burkholderia pyrrocinia (Bp); Pseudomonas fluorescens (Pf); Trichoderma asperellum (Ta) and a mixture of the three bioagents (Bp+Pf+Ta), in addition to untreated controls. The most effective treatment was silicon fertilization at 2tons in combination with the three bioagents, which reduced leaf blast by 96%. A second experiment was conducted to study the defense mechanism involved in disease suppression. The treatments were organized in a randomized block design, with or without Si (0 or 2 tons Si) and bioagents (non-inoculated control, Ta or Bp+Pf+Ta). The combination of silicon fertilization (2 tons SiCaMg.ha-1) with T. asperellum or with a mixture of the three bioagents increased the activities of chitinase (CHI), β-1,3glucanase (GLU), peroxidase (POX) and phenylalanine ammonia-lyase (PAL) as well as the salicylic acid (SA) content in rice plants in the absence of the pathogen Magnaporthe oryzae. The mixture of the three bioagents in plants fertilized with 2 tons Si increased GLU enzyme activity and SA levels at 24 and 48 hours after infection of the plants challenged with M. oryzae. The activation of defense mechanism by combination of silicon fertilization with bioagent mixture could be an effective strategy for rice blast management.
650 $aMagnaporthe oryzae
650 $aArroz
650 $aBrusone
650 $aDoença de planta
650 $aOryza sativa
650 $aSilício
653 $aBioagente
700 1 $aCORTES, M. V. de B.
700 1 $aSILVA, G. B. da
700 1 $aSOUSA, T. P. de
700 1 $aRODRIGUES, F. A.
700 1 $aFILIPPI, M. C. C.
773 $tInternational Journal of Research Studies in Biosciences$gv. 3, n. 5, p. 22-32, May 2015.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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