Data/hora: 06/06/2024 18:29:06

Biblioteca(s): 	Embrapa Agroenergia; Embrapa Milho e Sorgo.	
Data corrente: 	19/09/2017	
Data da última atualização: 	15/02/2018	
Autoria: 	SOUSA, C. A. F. de; PAIVA, D S. de; CASARI, R. A. das C. N.; MOLINARI, H. B. C.; OLIVEIRA, N. G. de; KOBAYASHI, A. K.; MAGALHAES, P. C.; GOMIDE, R. L.; SOUZA JUNIOR, M. T.	
Afiliação: 	CARLOS ANTONIO FERREIRA DE SOUSA, CNPAE; DAYANE SILVA DE PAIVA; RAPHAEL AUGUSTO DAS CHAGAS NOQUELI CASARI; HUGO BRUNO CORREA MOLINARI, CNPAE; NELSON GERALDO DE OLIVEIRA; ADILSON KENJI KOBAYASHI, CNPAE; PAULO CESAR MAGALHAES, CNPMS; REINALDO LUCIO GOMIDE, CNPMS; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE.	
Título: 	A procedure for maize genotypes discrimination to drought by chlorophyll fluorescence imaging rapid light curves.	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Plant Methods, v. 13, p. 1-17, 2017.	
DOI: 	10.1186/s13007-017-0209-z	
Idioma: 	Inglês	
Conteúdo: 	Background: Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the first process, has well-defined protocols. It is considered a standard method for evaluation of plant responses to drought. Under such stress, assessment of photochemical apparatus by chlorophyll fluorescence needs improvement to become faster and reproducible, especially in growing plants under field conditions. For this, we developed a protocol based on chlorophyll fluorescence imaging, using a rapid light curve approach. Results: Almost all parameters obtained by rapid light curves have shown statistical differences between control and drought stressed maize plants. However, most of them were affected by induction processes, relaxation rate, and/or differences in chlorophyll content; while they all were influenced by actinic light intensity on each light step of light curve. Only the normalized parameters related to photochemical and non-photochemical quenching were strongly correlated with data obtained by gas exchange, but only from the light step in which the linear electron flow reached saturation. Conclusions: The procedure developed in this study for discrimination of plant responses to water deficit stress proved to be as fast, efficient and reliable as the standard technique of gas exchange in order to discriminate the responses of maize genotypes to drought. However, unlike that, there is no need to perform daily and time consuming calibration routines. Moreover, plant acclimation to the dark is not required. The protocol can be applied to plants growing in both controlled conditions and full sunlight in the field. In addition, it generates parameters in a fast and accurate measurement process, which enables evaluating several plants in a short period of time.	
Palavras-Chave: 	Phenotyping; Water deficit; Zea mays L.	
Thesaurus Nal: 	abiotic stress; corn; gas exchange; phenomics.	
Categoria do assunto: 	--
F Plantas e Produtos de Origem Vegetal	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/168295/1/Procedure-maize.pdf	
Registro original: 	Embrapa Milho e Sorgo (CNPMS)	
Exemplares: 	CNPAE - (3179-1) - UPC - AP - DD -  -  -  - DIS
CNPMS - (28029-1) - UPC - AP - DD -  -  -  - DIS