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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/08/2002 |
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Data da última atualização: |
07/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOTA, F. F. da; NOBREGA, F. F.; MARRIEL, I. E.; PAIVA, E.; SELDIN, L. |
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Afiliação: |
IVANILDO EVODIO MARRIEL, CNPMS. |
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Título: |
Genetic diversity of Paenibacillus polymyxa populations isolated from the rhizosphere of four cultivars of maize (Zea mays) planted in cerrado soil. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Applied Soil Ecology, Amsterdam, v. 20, n. 2, p. 119-132, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
A tropical Brazilian soil (Cerrado) was planted with four cultivars of maize (CMS04, CMS11, CMS22 and CMS36) and the genetic diversity of the Paenibacillus polymyxa populations present in their rhizospheres was determined after 90 days of sowing. For that, a total of 67 isolates were identified as R polymyxa by classical biochemical tests and were analyzed for DNA polymorphism with the randomly amplified polymorphic DNA (RAPD) and amplification of repetitive DNA sequences (rep) methods. The amplification patterns obtained using three arbitrary primers and the primer BOXA1R were used separately to construct dendrograms based on the unweighted pair groups method with arithmetic means (UPGMA). Fifty-four genotypic groups were formed when data from different PCR amplification were combined, showing a high level of genetic polymorphism among P. polymyxa strains. A dendrogram based also on combined PCR data, followed by cluster analysis with minimum-variance criteria (Ward) and Euclidean distance, showed that P. polymyxa strains could be divided into two main clusters. One cluster was formed predominantly by strains from maize cultivars CMS04 and CMS36, while the other cluster was formed predominantly by strains of maize cultivars CMS11 and CMS22. Multivariate analysis of variance (MANOVA) allowed the correlation between the genetic structure of P. polymyxa populations and the different cultivars of maize to be studied. The results showed that the strains isolated from the rhizospheres of the different maize cultivars were significantly different. (C) 2002 Elsevier Science B. V. Alls rights reserved. MenosA tropical Brazilian soil (Cerrado) was planted with four cultivars of maize (CMS04, CMS11, CMS22 and CMS36) and the genetic diversity of the Paenibacillus polymyxa populations present in their rhizospheres was determined after 90 days of sowing. For that, a total of 67 isolates were identified as R polymyxa by classical biochemical tests and were analyzed for DNA polymorphism with the randomly amplified polymorphic DNA (RAPD) and amplification of repetitive DNA sequences (rep) methods. The amplification patterns obtained using three arbitrary primers and the primer BOXA1R were used separately to construct dendrograms based on the unweighted pair groups method with arithmetic means (UPGMA). Fifty-four genotypic groups were formed when data from different PCR amplification were combined, showing a high level of genetic polymorphism among P. polymyxa strains. A dendrogram based also on combined PCR data, followed by cluster analysis with minimum-variance criteria (Ward) and Euclidean distance, showed that P. polymyxa strains could be divided into two main clusters. One cluster was formed predominantly by strains from maize cultivars CMS04 and CMS36, while the other cluster was formed predominantly by strains of maize cultivars CMS11 and CMS22. Multivariate analysis of variance (MANOVA) allowed the correlation between the genetic structure of P. polymyxa populations and the different cultivars of maize to be studied. The results showed that the strains isolated from the rhizosp... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genetica. |
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Thesagro: |
Bactéria; Cerrado; Milho; Rizosfera. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/66483/1/Genetic-diversity-1.pdf
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Marc: |
LEADER 02330naa a2200229 a 4500
001 1485544
005 2018-06-07
008 2002 bl uuuu u00u1 u #d
100 1 $aMOTA, F. F. da
245 $aGenetic diversity of Paenibacillus polymyxa populations isolated from the rhizosphere of four cultivars of maize (Zea mays) planted in cerrado soil.$h[electronic resource]
260 $c2002
520 $aA tropical Brazilian soil (Cerrado) was planted with four cultivars of maize (CMS04, CMS11, CMS22 and CMS36) and the genetic diversity of the Paenibacillus polymyxa populations present in their rhizospheres was determined after 90 days of sowing. For that, a total of 67 isolates were identified as R polymyxa by classical biochemical tests and were analyzed for DNA polymorphism with the randomly amplified polymorphic DNA (RAPD) and amplification of repetitive DNA sequences (rep) methods. The amplification patterns obtained using three arbitrary primers and the primer BOXA1R were used separately to construct dendrograms based on the unweighted pair groups method with arithmetic means (UPGMA). Fifty-four genotypic groups were formed when data from different PCR amplification were combined, showing a high level of genetic polymorphism among P. polymyxa strains. A dendrogram based also on combined PCR data, followed by cluster analysis with minimum-variance criteria (Ward) and Euclidean distance, showed that P. polymyxa strains could be divided into two main clusters. One cluster was formed predominantly by strains from maize cultivars CMS04 and CMS36, while the other cluster was formed predominantly by strains of maize cultivars CMS11 and CMS22. Multivariate analysis of variance (MANOVA) allowed the correlation between the genetic structure of P. polymyxa populations and the different cultivars of maize to be studied. The results showed that the strains isolated from the rhizospheres of the different maize cultivars were significantly different. (C) 2002 Elsevier Science B. V. Alls rights reserved.
650 $aBactéria
650 $aCerrado
650 $aMilho
650 $aRizosfera
653 $aDiversidade genetica
700 1 $aNOBREGA, F. F.
700 1 $aMARRIEL, I. E.
700 1 $aPAIVA, E.
700 1 $aSELDIN, L.
773 $tApplied Soil Ecology, Amsterdam$gv. 20, n. 2, p. 119-132, 2002.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
10/11/2017 |
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Data da última atualização: |
10/11/2017 |
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Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
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Autoria: |
SOARES, S. M.; GASPAR, E. B.; MINHO, A. P.; SUÑÉ, R. W.; RIZZARDO, J. S. |
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Afiliação: |
Suelen Mendonça Soares, UPF; EMANUELLE BALDO GASPAR, CPPSUL; ALESSANDRO PELEGRINE MINHO, CPPSUL; RENATA WOLF SUNE MARTINS DA SILVA, CPPSUL; Juliana Soares Rizzardo, URCAMP. |
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Título: |
Caracterização da ocorrência de mastite subclínica no rebanho leiteiro da Embrapa Pecuária Sul. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Bagé: Embrapa Pecuária Sul, 2017. |
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Páginas: |
8 p. |
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Série: |
(Embrapa Pecuária Sul. Comunicado técnico, 92). |
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ISSN: |
1982-5382 |
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Idioma: |
Português |
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Conteúdo: |
O objetivo deste documento foi caracterizar a ocorrência da mastite subclínica, levando em consideração o número de lactações dos animais, bem como analisar a correlação de testes laboratoriais e a campo para o diagnóstico desta enfermidade no rebanho da Embrapa Pecuária Sul. |
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Thesagro: |
Doença animal; Gado leiteiro; Mamite; Teste de mamite. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166576/1/CoT-92-online.pdf
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Marc: |
LEADER 00997nam a2200241 a 4500
001 2079472
005 2017-11-10
008 2017 bl uuuu u0uu1 u #d
022 $a1982-5382
100 1 $aSOARES, S. M.
245 $aCaracterização da ocorrência de mastite subclínica no rebanho leiteiro da Embrapa Pecuária Sul.$h[electronic resource]
260 $aBagé: Embrapa Pecuária Sul$c2017
300 $a8 p.
490 $a(Embrapa Pecuária Sul. Comunicado técnico, 92).
520 $aO objetivo deste documento foi caracterizar a ocorrência da mastite subclínica, levando em consideração o número de lactações dos animais, bem como analisar a correlação de testes laboratoriais e a campo para o diagnóstico desta enfermidade no rebanho da Embrapa Pecuária Sul.
650 $aDoença animal
650 $aGado leiteiro
650 $aMamite
650 $aTeste de mamite
700 1 $aGASPAR, E. B.
700 1 $aMINHO, A. P.
700 1 $aSUÑÉ, R. W.
700 1 $aRIZZARDO, J. S.
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Embrapa Pecuária Sul (CPPSUL) |
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