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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
25/11/2022 |
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Data da última atualização: |
22/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FACCHINATTO, W. M.; ARAUJO, L. O.; MORAES, T. B.; ABELHA, T. F.; LIMA, T. H. N.; SANTOS, D. M. dos; CAMPANA-FILHO, S. P.; COLNAGO, L. A.; CAIRES, A. R. L. |
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Afiliação: |
LUIZ ALBERTO COLNAGO, CNPDIA. |
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Título: |
Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
International Journal of Molecular Sciences, v. 23, a12519, 2022. |
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Páginas: |
16 p. |
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DOI: |
https://doi.org/10.3390/ijms232012519 |
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Idioma: |
Inglês |
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Conteúdo: |
Multidrug-resistant bacteria represent a global health and economic burden that urgently calls for new technologies to combat bacterial antimicrobial resistance. Here, we developed novel nanocomposites (NCPs) based on chitosan that display different degrees of acetylation (DAs), and conjugated polymer cyano-substituted poly(p-phenylene vinylene) (CNPPV) as an alternative approach to inactivate Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria. Chitosan?s structure was confirmed through FT-Raman spectroscopy. Bactericidal and photobactericidal activities of NCPs were tested under dark and blue-light irradiation conditions, respectively. Hydrodynamic size and aqueous stability were determined by DLS, zeta potential (ZP) and time-domain NMR. TEM micrographs of NCPs were obtained, and their capacity of generating reactive oxygen species(ROS) under blue illumination was also characterized. Meaningful variations on ZP and relaxationtime T2 confirmed successful physical attachment of chitosan/CNPPV. All NCPs exhibited a similarand shrunken spherical shape according to TEM. A lower DA is responsible for driving higher bactericidal performance alongside the synergistic effect from CNPPV, lower nanosized distribution profile and higher positive charged surface. ROS production was proportionally found in NCPs with and without CNPPV by decreasing the DA, leading to a remarkable photobactericidal effect underblue-light irradiation. Overall, our findings indicate that chitosan/CNPPV NCPs may constitute a valuable asset for the development of innovative strategies for inactivation and/or photoinactivation of bacteria MenosMultidrug-resistant bacteria represent a global health and economic burden that urgently calls for new technologies to combat bacterial antimicrobial resistance. Here, we developed novel nanocomposites (NCPs) based on chitosan that display different degrees of acetylation (DAs), and conjugated polymer cyano-substituted poly(p-phenylene vinylene) (CNPPV) as an alternative approach to inactivate Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria. Chitosan?s structure was confirmed through FT-Raman spectroscopy. Bactericidal and photobactericidal activities of NCPs were tested under dark and blue-light irradiation conditions, respectively. Hydrodynamic size and aqueous stability were determined by DLS, zeta potential (ZP) and time-domain NMR. TEM micrographs of NCPs were obtained, and their capacity of generating reactive oxygen species(ROS) under blue illumination was also characterized. Meaningful variations on ZP and relaxationtime T2 confirmed successful physical attachment of chitosan/CNPPV. All NCPs exhibited a similarand shrunken spherical shape according to TEM. A lower DA is responsible for driving higher bactericidal performance alongside the synergistic effect from CNPPV, lower nanosized distribution profile and higher positive charged surface. ROS production was proportionally found in NCPs with and without CNPPV by decreasing the DA, leading to a remarkable photobactericidal effect underblue-light irradiation. Overall, our findings indicate that chitosa... Mostrar Tudo |
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Palavras-Chave: |
Blue-light irradiation; CNPPV; Photoantimicrobial activity; S aureus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148795/1/p-Antimicrobial-and-Photoantimicrobial-Activities-of.pdf
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Marc: |
LEADER 02507naa a2200289 a 4500
001 2148795
005 2024-01-22
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/ijms232012519$2DOI
100 1 $aFACCHINATTO, W. M.
245 $aAntimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites.$h[electronic resource]
260 $c2022
300 $a16 p.
520 $aMultidrug-resistant bacteria represent a global health and economic burden that urgently calls for new technologies to combat bacterial antimicrobial resistance. Here, we developed novel nanocomposites (NCPs) based on chitosan that display different degrees of acetylation (DAs), and conjugated polymer cyano-substituted poly(p-phenylene vinylene) (CNPPV) as an alternative approach to inactivate Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria. Chitosan?s structure was confirmed through FT-Raman spectroscopy. Bactericidal and photobactericidal activities of NCPs were tested under dark and blue-light irradiation conditions, respectively. Hydrodynamic size and aqueous stability were determined by DLS, zeta potential (ZP) and time-domain NMR. TEM micrographs of NCPs were obtained, and their capacity of generating reactive oxygen species(ROS) under blue illumination was also characterized. Meaningful variations on ZP and relaxationtime T2 confirmed successful physical attachment of chitosan/CNPPV. All NCPs exhibited a similarand shrunken spherical shape according to TEM. A lower DA is responsible for driving higher bactericidal performance alongside the synergistic effect from CNPPV, lower nanosized distribution profile and higher positive charged surface. ROS production was proportionally found in NCPs with and without CNPPV by decreasing the DA, leading to a remarkable photobactericidal effect underblue-light irradiation. Overall, our findings indicate that chitosan/CNPPV NCPs may constitute a valuable asset for the development of innovative strategies for inactivation and/or photoinactivation of bacteria
653 $aBlue-light irradiation
653 $aCNPPV
653 $aPhotoantimicrobial activity
653 $aS aureus
700 1 $aARAUJO, L. O.
700 1 $aMORAES, T. B.
700 1 $aABELHA, T. F.
700 1 $aLIMA, T. H. N.
700 1 $aSANTOS, D. M. dos
700 1 $aCAMPANA-FILHO, S. P.
700 1 $aCOLNAGO, L. A.
700 1 $aCAIRES, A. R. L.
773 $tInternational Journal of Molecular Sciences$gv. 23, a12519, 2022.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
26/03/2009 |
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Data da última atualização: |
26/03/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MIHSEN, N. R.; SOARES, R. P.; RAMIRO, C. A.; QUEIROZ, P. R.; LIMA, L. H. C.; MONNERAT, R. G. |
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Afiliação: |
N. R. Mihsen, UniCEUB; R. P. Soares, UniCEUB; C. A. Ramiro, UNB; P. R. Queiroz, UniCEUB; Luzia Helena Corrêa Lima, Embrapa Recursos Genéticos e Biotecnologia; Rose Gomes Monnerat Solon de Pontes, Embrapa Recursos Genéticos e Biotecnologia. |
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Título: |
Identificação de estirpes de Bacillus thuringiensis produtoras de bacteriocinas. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 089. |
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Páginas: |
p. 134. |
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Idioma: |
Português |
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Palavras-Chave: |
Bioinseticida. |
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Thesagro: |
Bacillus Thuringiensis; Controle Biológico. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00773nam a2200205 a 4500
001 1191165
005 2009-03-26
008 2008 bl uuuu u01u1 u #d
100 1 $aMIHSEN, N. R.
245 $aIdentificação de estirpes de Bacillus thuringiensis produtoras de bacteriocinas.
260 $aIn: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 089.$c2008
300 $ap. 134.
650 $aBacillus Thuringiensis
650 $aControle Biológico
653 $aBioinseticida
700 1 $aSOARES, R. P.
700 1 $aRAMIRO, C. A.
700 1 $aQUEIROZ, P. R.
700 1 $aLIMA, L. H. C.
700 1 $aMONNERAT, R. G.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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