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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
20/01/2006 |
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Data da última atualização: |
17/11/2015 |
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Autoria: |
CARNEIRO, N. P.; GUIMARAES, C. T.; MAGALHAES, J. V. |
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Afiliação: |
NEWTON PORTILHO CARNEIRO, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; JURANDIR VIEIRA DE MAGALHAES, CNPMS. |
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Título: |
Tipos de marcadores e genômica de plantas. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Bioscience Journal, Uberlândia, v. 20, p. 119-132, 2004. |
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Idioma: |
Português |
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Notas: |
Suplementt, 1. |
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Conteúdo: |
Plant breeding relies on genetic variation and selection to gradually improve plants for traits that are of interest to the grower and the consumer. This variability has been in part assessed through an efficient use of the available germplasm. Although breeding efforts have continuously produced improved varieties throughout the years, recent developments in the field of biotechnology and molecular biology can now be employed to facilitate and speed up cultivar development. These methods are mainly DNA-based techniques and basically use the hybridization of cloned probes to detect genomic sequences that are polymorphic for internal restriction sites, such as in restriction fragment length polymorphisms, or on the amplification of DNA fragments using the polymerase chain reaction (e.g. random amplified polymorphic DNA or simple sequence repeats (or microsatellites)). The amplified fragment length polymorphism method is conceptually a hybrid technique, relying on polymorphisms in the recognition sequences of restriction enzymes that are revealed through selective PCR amplification of adapter sequences. These techniques, when integrated with other resources such as mutants generated by chemical and insertional mutagenesis, quantitative trait locus mapping, cDNA sequencing projects, high throughput mass spectrometry and proteomics, physical and comparative mapping, have made gene cloning far more efficient. Advanced bioinformatic tools for data retrieval and subsequent statistical analysis have been developed, allowing for a quick and reliable management of enormous amount of data obtained in several plant species. The objective of the present manuscript is to offer an overview of the most common molecular markers used in the genetic analysis of traits that are relevant as targets for crop improvement. The advantages and disadvantages of current marker technologies are also discussed. MenosPlant breeding relies on genetic variation and selection to gradually improve plants for traits that are of interest to the grower and the consumer. This variability has been in part assessed through an efficient use of the available germplasm. Although breeding efforts have continuously produced improved varieties throughout the years, recent developments in the field of biotechnology and molecular biology can now be employed to facilitate and speed up cultivar development. These methods are mainly DNA-based techniques and basically use the hybridization of cloned probes to detect genomic sequences that are polymorphic for internal restriction sites, such as in restriction fragment length polymorphisms, or on the amplification of DNA fragments using the polymerase chain reaction (e.g. random amplified polymorphic DNA or simple sequence repeats (or microsatellites)). The amplified fragment length polymorphism method is conceptually a hybrid technique, relying on polymorphisms in the recognition sequences of restriction enzymes that are revealed through selective PCR amplification of adapter sequences. These techniques, when integrated with other resources such as mutants generated by chemical and insertional mutagenesis, quantitative trait locus mapping, cDNA sequencing projects, high throughput mass spectrometry and proteomics, physical and comparative mapping, have made gene cloning far more efficient. Advanced bioinformatic tools for data retrieval and subsequent statisti... Mostrar Tudo |
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Palavras-Chave: |
Melhoramento de plantas. |
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Thesagro: |
Biologia Molecular; Genética Molecular. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/79483/1/Tipos-marcadores.pdf
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Marc: |
LEADER 02471naa a2200193 a 4500
001 1489101
005 2015-11-17
008 2004 bl uuuu u00u1 u #d
100 1 $aCARNEIRO, N. P.
245 $aTipos de marcadores e genômica de plantas.$h[electronic resource]
260 $c2004
500 $aSuplementt, 1.
520 $aPlant breeding relies on genetic variation and selection to gradually improve plants for traits that are of interest to the grower and the consumer. This variability has been in part assessed through an efficient use of the available germplasm. Although breeding efforts have continuously produced improved varieties throughout the years, recent developments in the field of biotechnology and molecular biology can now be employed to facilitate and speed up cultivar development. These methods are mainly DNA-based techniques and basically use the hybridization of cloned probes to detect genomic sequences that are polymorphic for internal restriction sites, such as in restriction fragment length polymorphisms, or on the amplification of DNA fragments using the polymerase chain reaction (e.g. random amplified polymorphic DNA or simple sequence repeats (or microsatellites)). The amplified fragment length polymorphism method is conceptually a hybrid technique, relying on polymorphisms in the recognition sequences of restriction enzymes that are revealed through selective PCR amplification of adapter sequences. These techniques, when integrated with other resources such as mutants generated by chemical and insertional mutagenesis, quantitative trait locus mapping, cDNA sequencing projects, high throughput mass spectrometry and proteomics, physical and comparative mapping, have made gene cloning far more efficient. Advanced bioinformatic tools for data retrieval and subsequent statistical analysis have been developed, allowing for a quick and reliable management of enormous amount of data obtained in several plant species. The objective of the present manuscript is to offer an overview of the most common molecular markers used in the genetic analysis of traits that are relevant as targets for crop improvement. The advantages and disadvantages of current marker technologies are also discussed.
650 $aBiologia Molecular
650 $aGenética Molecular
653 $aMelhoramento de plantas
700 1 $aGUIMARAES, C. T.
700 1 $aMAGALHAES, J. V.
773 $tBioscience Journal, Uberlândia$gv. 20, p. 119-132, 2004.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
17/02/2014 |
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Data da última atualização: |
17/02/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
VIEIRA, A. S.; ROSINHA, G. M. S.; VASCONCELLOS, S. A.; MORAIS, Z. M. DE.; VIANA, R. C.; OLIVEIRA, C. E. DE.; SOARES, C. O.; ARAUJO, F. R.; MOURAO, G. de M.; BIANCHI, R. DE C.; OLIFIERS, N.; RADEMAKER, V.; ROCHA, F. L.; PELLEGRIN, A. O. |
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Título: |
Identificação de mamíferos silvestres do Pantanal sul-mato-grossense portadores de Leptospira spp. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Ciência Animal Brasileira v. 14, n. 3, p. 373-380, 2013. |
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Páginas: |
8p. |
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ISSN: |
1518-2797 |
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Idioma: |
Português |
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Conteúdo: |
Foi realizado um levantamento da infecção por Leptospira spp. em mamíferos silvestres do Pantanal sul-matogrossense com o emprego da reação de soroaglutinação microscópica (SAM) e da reação em cadeia da polimerase (PCR). Os sorovares de maior frequência nos animais investigados foram Hardjobovis (28%), Icterohemorhagiae (12%), M-110/2006 (isolado de Cerdocyon thous; 16%), Canicola (L014 isolada de Bos taurus, 4%), Whitcombi (4%), Pomona (20%), Autumnalis (12%) e Copenhageni (M9/99 isolada de Rattus norvegicus, 4%). Das 79 amostras examinadas pela PCR, 21 (26,58%) foram positivas, com a amplificação de um fragmento de aproximadamente 331pb. Dois fragmentos amplificados obtidos de amostras de C. thous foram clonados, sequenciados e identificados como L. interrogans por análise filogenética. A survey of Leptospira spp. in wild mammals from the southern Pantanal of Mato Grosso do Sul was performed by microscopic agglutination test (MAT) and polymerase chain reaction (PCR). The serovars most frequently found were Hardjobovis (28%), Icterohemorhagiae (12%), M110/2006 strain (isolated from Cerdocyon thous, 16%), Canicola (L014 isolated from Bos Taurus, 4%), Whitcombi (4%), Pomona (20%), Autumnalis (12%) and Copenhageni (M9/99 isolated from Rattus norvegicus, 4%). From the 79 samples tested by PCR, 21 (26.58%) were positive, resulting in the amplification fragment of approximately 331pb. Two amplified fragments from C. thous were cloned, sequenced and identified as L. interrogans by phylogenetic analysis. MenosFoi realizado um levantamento da infecção por Leptospira spp. em mamíferos silvestres do Pantanal sul-matogrossense com o emprego da reação de soroaglutinação microscópica (SAM) e da reação em cadeia da polimerase (PCR). Os sorovares de maior frequência nos animais investigados foram Hardjobovis (28%), Icterohemorhagiae (12%), M-110/2006 (isolado de Cerdocyon thous; 16%), Canicola (L014 isolada de Bos taurus, 4%), Whitcombi (4%), Pomona (20%), Autumnalis (12%) e Copenhageni (M9/99 isolada de Rattus norvegicus, 4%). Das 79 amostras examinadas pela PCR, 21 (26,58%) foram positivas, com a amplificação de um fragmento de aproximadamente 331pb. Dois fragmentos amplificados obtidos de amostras de C. thous foram clonados, sequenciados e identificados como L. interrogans por análise filogenética. A survey of Leptospira spp. in wild mammals from the southern Pantanal of Mato Grosso do Sul was performed by microscopic agglutination test (MAT) and polymerase chain reaction (PCR). The serovars most frequently found were Hardjobovis (28%), Icterohemorhagiae (12%), M110/2006 strain (isolated from Cerdocyon thous, 16%), Canicola (L014 isolated from Bos Taurus, 4%), Whitcombi (4%), Pomona (20%), Autumnalis (12%) and Copenhageni (M9/99 isolated from Rattus norvegicus, 4%). From the 79 samples tested by PCR, 21 (26.58%) were positive, resulting in the amplification fragment of approximately 331pb. Two amplified fragments from C. thous were cloned, sequenced and identified as L. interrogans by... Mostrar Tudo |
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Palavras-Chave: |
Mamíferos silvestres; PCR; Sorologia; Wild mammals. |
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Thesagro: |
Leptospirose. |
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Thesaurus NAL: |
leptospirosis; Pantanal; serology. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97494/1/15-17147-2013-Leptospira-Mamiferos.pdf
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Marc: |
LEADER 02628naa a2200397 a 4500
001 1980108
005 2014-02-17
008 2013 bl uuuu u00u1 u #d
022 $a1518-2797
100 1 $aVIEIRA, A. S.
245 $aIdentificação de mamíferos silvestres do Pantanal sul-mato-grossense portadores de Leptospira spp.$h[electronic resource]
260 $c2013
300 $a8p.
520 $aFoi realizado um levantamento da infecção por Leptospira spp. em mamíferos silvestres do Pantanal sul-matogrossense com o emprego da reação de soroaglutinação microscópica (SAM) e da reação em cadeia da polimerase (PCR). Os sorovares de maior frequência nos animais investigados foram Hardjobovis (28%), Icterohemorhagiae (12%), M-110/2006 (isolado de Cerdocyon thous; 16%), Canicola (L014 isolada de Bos taurus, 4%), Whitcombi (4%), Pomona (20%), Autumnalis (12%) e Copenhageni (M9/99 isolada de Rattus norvegicus, 4%). Das 79 amostras examinadas pela PCR, 21 (26,58%) foram positivas, com a amplificação de um fragmento de aproximadamente 331pb. Dois fragmentos amplificados obtidos de amostras de C. thous foram clonados, sequenciados e identificados como L. interrogans por análise filogenética. A survey of Leptospira spp. in wild mammals from the southern Pantanal of Mato Grosso do Sul was performed by microscopic agglutination test (MAT) and polymerase chain reaction (PCR). The serovars most frequently found were Hardjobovis (28%), Icterohemorhagiae (12%), M110/2006 strain (isolated from Cerdocyon thous, 16%), Canicola (L014 isolated from Bos Taurus, 4%), Whitcombi (4%), Pomona (20%), Autumnalis (12%) and Copenhageni (M9/99 isolated from Rattus norvegicus, 4%). From the 79 samples tested by PCR, 21 (26.58%) were positive, resulting in the amplification fragment of approximately 331pb. Two amplified fragments from C. thous were cloned, sequenced and identified as L. interrogans by phylogenetic analysis.
650 $aleptospirosis
650 $aPantanal
650 $aserology
650 $aLeptospirose
653 $aMamíferos silvestres
653 $aPCR
653 $aSorologia
653 $aWild mammals
700 1 $aROSINHA, G. M. S.
700 1 $aVASCONCELLOS, S. A.
700 1 $aMORAIS, Z. M. DE.
700 1 $aVIANA, R. C.
700 1 $aOLIVEIRA, C. E. DE.
700 1 $aSOARES, C. O.
700 1 $aARAUJO, F. R.
700 1 $aMOURAO, G. de M.
700 1 $aBIANCHI, R. DE C.
700 1 $aOLIFIERS, N.
700 1 $aRADEMAKER, V.
700 1 $aROCHA, F. L.
700 1 $aPELLEGRIN, A. O.
773 $tCiência Animal Brasileira$gv. 14, n. 3, p. 373-380, 2013.
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