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Registro Completo |
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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
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Data corrente: |
29/09/2023 |
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Data da última atualização: |
29/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PRADO, C.; PEREIRA, R.; DURRANT, L.; SCORZA JUNIOR, R. P.; PIUBELI, F.; BONFÁ, M. |
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Afiliação: |
CAIO PRADO, UNIVERSIDADE DE SÃO PAULO, FACULDADE DE ENGENHARIA, LORENA, SP; RODRIGO PEREIRA, UNIVERSIDADE FEDERAL DA GRANDE DOURADOS, DOURADOS; LUCIA DURRANT, BioSage, Lawreceville, NJ, USA; ROMULO PENNA SCORZA JUNIOR, CPAO; FRANCINE PIUBELI, University of Seville, Pharmacy Faculty, Seville, Spain; MARICI BONFÁ, UNIVERSIDADE FEDERAL DA GRANDE DOURADOS, DOURADOS. |
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Título: |
Fipronil Degradation in Soil by Enterobacter chengduensis Strain G2.8: Metabolic Perspective. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Life, v. 13, n. 9, p. 1935, 2023. |
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DOI: |
10.3390/life13091935 |
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Idioma: |
Inglês |
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Conteúdo: |
Fipronil is an insecticide widely used in the agricultural and veterinary sectors for its efficacy in pest control. The presence of fipronil in the environment is mainly due to agricultural and domestic practices and is frequently found in different types of environmental matrices in concentrations ranging from µg/L to mg/L and can be hazardous to non-target organisms due to its high toxicity. This study was carried out to obtain and characterize microorganisms from soil which are capable of biodegrading fipronil that could be of great biotechnological interest. For this purpose, bioprospecting was carried out using fipronil (0.6 g/L) as the main source of carbon and nitrogen for growth. Once obtained, the strain was identified by sequencing the 16S ribosomal RNA (rRNA) gene and the capacity to degrade fipronil was monitored by GC-MS. Our study showed a presence in soil samples of the strain identified as Enterobacter chengduensis, which was able to metabolize fipronil and its metabolites during the mineralization process. Enterobacter chengduensis was able to biodegrade fipronil (96%) and its metabolites fipronil-sulfone (92%) and fipronil-sulfide (79%) in 14 days. Overall, the results of this study provided a bacterium with great potential that could contribute to the degradation of fipronil in the environment. |
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Thesagro: |
Biodegradação; Pesticida. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156992/1/ARTIGO.pdf
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Marc: |
LEADER 01952naa a2200217 a 4500
001 2156992
005 2023-09-29
008 2023 bl uuuu u00u1 u #d
024 7 $a10.3390/life13091935$2DOI
100 1 $aPRADO, C.
245 $aFipronil Degradation in Soil by Enterobacter chengduensis Strain G2.8$bMetabolic Perspective.$h[electronic resource]
260 $c2023
520 $aFipronil is an insecticide widely used in the agricultural and veterinary sectors for its efficacy in pest control. The presence of fipronil in the environment is mainly due to agricultural and domestic practices and is frequently found in different types of environmental matrices in concentrations ranging from µg/L to mg/L and can be hazardous to non-target organisms due to its high toxicity. This study was carried out to obtain and characterize microorganisms from soil which are capable of biodegrading fipronil that could be of great biotechnological interest. For this purpose, bioprospecting was carried out using fipronil (0.6 g/L) as the main source of carbon and nitrogen for growth. Once obtained, the strain was identified by sequencing the 16S ribosomal RNA (rRNA) gene and the capacity to degrade fipronil was monitored by GC-MS. Our study showed a presence in soil samples of the strain identified as Enterobacter chengduensis, which was able to metabolize fipronil and its metabolites during the mineralization process. Enterobacter chengduensis was able to biodegrade fipronil (96%) and its metabolites fipronil-sulfone (92%) and fipronil-sulfide (79%) in 14 days. Overall, the results of this study provided a bacterium with great potential that could contribute to the degradation of fipronil in the environment.
650 $aBiodegradação
650 $aPesticida
700 1 $aPEREIRA, R.
700 1 $aDURRANT, L.
700 1 $aSCORZA JUNIOR, R. P.
700 1 $aPIUBELI, F.
700 1 $aBONFÁ, M.
773 $tLife$gv. 13, n. 9, p. 1935, 2023.
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Registro original: |
Embrapa Agropecuária Oeste (CPAO) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
18/03/2022 |
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Data da última atualização: |
18/03/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SOARES, J. C.; SOARES, A. C.; ANGELIM, M. K. S. C.; PROENÇA-MODENA, J. L.; VIEIRA, P. M. M.; MATTOSO, L. H. C.; OLIVEIRA JR, O. N. |
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Afiliação: |
LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
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Título: |
Diagnostics of SARS-CoV-2 infection using electrical impedance spectroscopy with an immunosensor to detect the spike protein. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Talanta, v. 239, 123076, 2022. |
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Páginas: |
1 - 7 |
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ISSN: |
0039-9140 |
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DOI: |
https://doi.org/10.1016/j.talanta.2021.123076 |
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Idioma: |
Inglês |
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Conteúdo: |
Mass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing even in places with limited resources. MenosMass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be ap... Mostrar Tudo |
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Palavras-Chave: |
Impedance spectroscopy; Information visualization; SARS-CoV-2; Spike protein. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02452naa a2200277 a 4500
001 2141031
005 2022-03-18
008 2022 bl uuuu u00u1 u #d
022 $a0039-9140
024 7 $ahttps://doi.org/10.1016/j.talanta.2021.123076$2DOI
100 1 $aSOARES, J. C.
245 $aDiagnostics of SARS-CoV-2 infection using electrical impedance spectroscopy with an immunosensor to detect the spike protein.$h[electronic resource]
260 $c2022
300 $a1 - 7
520 $aMass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing even in places with limited resources.
653 $aImpedance spectroscopy
653 $aInformation visualization
653 $aSARS-CoV-2
653 $aSpike protein
700 1 $aSOARES, A. C.
700 1 $aANGELIM, M. K. S. C.
700 1 $aPROENÇA-MODENA, J. L.
700 1 $aVIEIRA, P. M. M.
700 1 $aMATTOSO, L. H. C.
700 1 $aOLIVEIRA JR, O. N.
773 $tTalanta$gv. 239, 123076, 2022.
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