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| 1. |  | RAMOS-GONZALEZ, P. L.; CHABI, C.; KITAJIMA, E. W.; ASTUA, J. de F. Degenerate primers for the detection of tentative dichorhaviruses associated to citrus leprosis disease. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 48.; CONGRESSO BRASILEIRO DE PATOLOGIA PÓS-COLHEITA, 2., 2015, São Pedro. Fitopatologia de precisão: fronteiras da ciência: anais. Brasília, DF: Sociedade Brasileira de Fitopatologia, 2015. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | RAMOS-GONZÁLEZ, P. L.; KONDO, H.; MOROZOV, S.; VASILAKIS, N.; VARSANI, A.; CAO, M.; ASTUA, J. de F. The Border between kitavirids and nege-like viruses: tracking the evolutionary pace of plant- and arthropod-infecting viruses. Frontiers in Plant Science, v.3, 932523, May, 2022. doi: il. PDF.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
16/05/2016 |
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Data da última atualização: |
16/02/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
DAMASCENO, J. C. A.; SOARES, A. C. F.; JESUS, F. N. de; CASTRO, J. M. da C. e. |
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Afiliação: |
JOSILDA CAVALCANTE AMORIM DAMASCENO, Universidade Federal do Recôncavo da Bahia - UFRB, Cruz das Almas BA; ANA CRISTINA FERMINO SOARES, Universidade Federal do Recôncavo da Bahia - UFRB, Cruz das Almas, BA; FÁBIO NASCIMENTO DE JESUS, Universidade Federal do Recôncavo da Bahia - UFRB, Cruz das Almas, BA; JOSE MAURO DA CUNHA E CASTRO, CPATSA. |
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Título: |
Root-knot nematode staining with artificial food dyes. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Nematoda, v. 3, 2016. |
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DOI: |
10.4322/nematoda.01816 |
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Idioma: |
Inglês |
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Conteúdo: |
The dyes used for staining nematode egg masses and eggs can cause environmental hazards and human health concerns. The aim of this study was to evaluate different artificial food dyes, as a replacement for acid fuchsin and phloxine B, for staining eggs and egg masses of Meloidogyne javanica from infected tomato plants. Five different combinations of dyes were tested at five concentrations. For control treatments, infected roots were stained with acid fuchsin and phloxine B for coloring eggs and egg masses, respectively. Egg staining with the dyes ponceau 4R with red 40 and ponceau 4R with brilliant blue, at the concentration of 4%, showed results similar to those obtained with acid fuchsin. However, egg masses were effectively stained with ponceau 4R food dyes with red 40 and with ponceau 4R with brilliant blue at all tested concentrations. Ponceau food coloring dyes gave a good contrast for nematode egg masses and egg counting, do not present human health and environmental pollution problems, and can be reused, with less waste being generated in the laboratory. These dyes are low-cost products that are available at most supermarkets, and can efficiently substitute acid fuchsin and phloxine B for staining eggs and egg masses of M. javanica. |
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Palavras-Chave: |
Coloração; Corantes alimentares artificiais; Insect; Massas de ovos; Nematoide das galhas; Ovos de nematóides. |
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Thesagro: |
Inseto; Meloidogyne Javanica; Nematoide. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143041/1/Jose-Mauro-2016.pdf
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Marc: |
LEADER 02025naa a2200277 a 4500
001 2045048
005 2017-02-16
008 2016 bl uuuu u00u1 u #d
024 7 $a10.4322/nematoda.01816$2DOI
100 1 $aDAMASCENO, J. C. A.
245 $aRoot-knot nematode staining with artificial food dyes.$h[electronic resource]
260 $c2016
520 $aThe dyes used for staining nematode egg masses and eggs can cause environmental hazards and human health concerns. The aim of this study was to evaluate different artificial food dyes, as a replacement for acid fuchsin and phloxine B, for staining eggs and egg masses of Meloidogyne javanica from infected tomato plants. Five different combinations of dyes were tested at five concentrations. For control treatments, infected roots were stained with acid fuchsin and phloxine B for coloring eggs and egg masses, respectively. Egg staining with the dyes ponceau 4R with red 40 and ponceau 4R with brilliant blue, at the concentration of 4%, showed results similar to those obtained with acid fuchsin. However, egg masses were effectively stained with ponceau 4R food dyes with red 40 and with ponceau 4R with brilliant blue at all tested concentrations. Ponceau food coloring dyes gave a good contrast for nematode egg masses and egg counting, do not present human health and environmental pollution problems, and can be reused, with less waste being generated in the laboratory. These dyes are low-cost products that are available at most supermarkets, and can efficiently substitute acid fuchsin and phloxine B for staining eggs and egg masses of M. javanica.
650 $aInseto
650 $aMeloidogyne Javanica
650 $aNematoide
653 $aColoração
653 $aCorantes alimentares artificiais
653 $aInsect
653 $aMassas de ovos
653 $aNematoide das galhas
653 $aOvos de nematóides
700 1 $aSOARES, A. C. F.
700 1 $aJESUS, F. N. de
700 1 $aCASTRO, J. M. da C. e
773 $tNematoda$gv. 3, 2016.
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