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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
09/03/2010 |
Data da última atualização: |
26/04/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SOUZA, R. C. P.; LOBO JUNIOR, M.; CORRÊA, C. A. |
Afiliação: |
R. C. P. SOUZA, UFG; MURILLO LOBO JUNIOR, CNPAF; C. A. CORRÊA, UFG. |
Título: |
Efeito de Pyribencarb sobre apotécios de Sclerotinia sclerotiorum. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Tropical Plant Pathology, Brasília, DF, v. 34, p. S110, ago. 2009. Suplemento, ref. 404. Edição dos Resumos do XLII do Congresso Brasileiro de Fitopatologia, Rio de Janeiro, RJ, ago. 2009. |
ISSN: |
1982-5676 |
Idioma: |
Português |
Thesagro: |
Doença de planta; Feijão; Fungicida; Mofo branco; Phaseolus vulgaris; Sclerotinia sclerotiorum. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/660307/1/cbf-2009-pS110.pdf
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Marc: |
LEADER 00752nam a2200205 a 4500 001 1660307 005 2023-04-26 008 2009 bl uuuu u00u1 u #d 022 $a1982-5676 100 1 $aSOUZA, R. C. P. 245 $aEfeito de Pyribencarb sobre apotécios de Sclerotinia sclerotiorum.$h[electronic resource] 260 $aTropical Plant Pathology, Brasília, DF, v. 34, p. S110, ago. 2009. Suplemento, ref. 404. Edição dos Resumos do XLII do Congresso Brasileiro de Fitopatologia, Rio de Janeiro, RJ, ago. 2009.$c2009 650 $aDoença de planta 650 $aFeijão 650 $aFungicida 650 $aMofo branco 650 $aPhaseolus vulgaris 650 $aSclerotinia sclerotiorum 700 1 $aLOBO JUNIOR, M. 700 1 $aCORRÊA, C. A.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
06/02/2017 |
Data da última atualização: |
30/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SIQUEIRA, L. G. B.; HANSEN, P. J. |
Afiliação: |
LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; PETER J. HANSEN, University of Florida, Gainesville, Florida, USA. |
Título: |
Sex differences in response of the bovine embryo to colony-stimulating factor 2. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Reproduction, n. 152, p. 645-654, 2016. |
Idioma: |
Inglês |
Conteúdo: |
We tested whether gene expression of the bovine morula is modified by CSF2 in a sex-dependent manner and if sex determines the effect of CSF2 on competence of embryos to become blastocysts. Embryos were produced in vitro using X- or Y-sorted semen and treated at Day 5 of culture with 10 ng/mL bovine CSF2 or control. In experiment 1, morulae were collected at Day 6 and biological replicates (n = 8) were evaluated for transcript abundance of 90 genes by RT-qPCR using the Fluidigm Delta Gene assay. Expression of more than one-third (33 of 90) of genes examined was affected by sex. The effect of CSF2 on gene expression was modified by sex (P < 0.05) for five genes (DDX3Y/DDX3X-like, NANOG, MYF6, POU5F1 and RIPK3) and tended (P < 0.10) to be modified by sex for five other genes (DAPK1, HOXA5, PPP2R3A, PTEN and TNFSF8). In experiment 2, embryos were treated at Day 5 with control or CSF2 and blastocysts were collected at Day 7 for immunolabeling to determine the number of inner cell mass (ICM) and trophectoderm (TE) cells. CSF2 increased the percent of putative zygotes that became blastocysts for females, but did not affect the development of males. There was no effect of CSF2 or interaction of CSF2 with sex on the total number of blastomeres in blastocysts or in the number of inner cell mass or trophectoderm cells. In conclusion, CSF2 exerted divergent responses on gene expression and development of female and male embryos. These results are evidence of sexually dimorphic responses of the preimplantation embryo to this embryokine. MenosWe tested whether gene expression of the bovine morula is modified by CSF2 in a sex-dependent manner and if sex determines the effect of CSF2 on competence of embryos to become blastocysts. Embryos were produced in vitro using X- or Y-sorted semen and treated at Day 5 of culture with 10 ng/mL bovine CSF2 or control. In experiment 1, morulae were collected at Day 6 and biological replicates (n = 8) were evaluated for transcript abundance of 90 genes by RT-qPCR using the Fluidigm Delta Gene assay. Expression of more than one-third (33 of 90) of genes examined was affected by sex. The effect of CSF2 on gene expression was modified by sex (P < 0.05) for five genes (DDX3Y/DDX3X-like, NANOG, MYF6, POU5F1 and RIPK3) and tended (P < 0.10) to be modified by sex for five other genes (DAPK1, HOXA5, PPP2R3A, PTEN and TNFSF8). In experiment 2, embryos were treated at Day 5 with control or CSF2 and blastocysts were collected at Day 7 for immunolabeling to determine the number of inner cell mass (ICM) and trophectoderm (TE) cells. CSF2 increased the percent of putative zygotes that became blastocysts for females, but did not affect the development of males. There was no effect of CSF2 or interaction of CSF2 with sex on the total number of blastomeres in blastocysts or in the number of inner cell mass or trophectoderm cells. In conclusion, CSF2 exerted divergent responses on gene expression and development of female and male embryos. These results a... Mostrar Tudo |
Palavras-Chave: |
CSF2; Embryokine. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154808/1/Cnpgl-2016-Reproduction-Sex.pdf
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Marc: |
LEADER 02045naa a2200157 a 4500 001 2062824 005 2023-01-30 008 2016 bl uuuu u00u1 u #d 100 1 $aSIQUEIRA, L. G. B. 245 $aSex differences in response of the bovine embryo to colony-stimulating factor 2.$h[electronic resource] 260 $c2016 520 $aWe tested whether gene expression of the bovine morula is modified by CSF2 in a sex-dependent manner and if sex determines the effect of CSF2 on competence of embryos to become blastocysts. Embryos were produced in vitro using X- or Y-sorted semen and treated at Day 5 of culture with 10 ng/mL bovine CSF2 or control. In experiment 1, morulae were collected at Day 6 and biological replicates (n = 8) were evaluated for transcript abundance of 90 genes by RT-qPCR using the Fluidigm Delta Gene assay. Expression of more than one-third (33 of 90) of genes examined was affected by sex. The effect of CSF2 on gene expression was modified by sex (P < 0.05) for five genes (DDX3Y/DDX3X-like, NANOG, MYF6, POU5F1 and RIPK3) and tended (P < 0.10) to be modified by sex for five other genes (DAPK1, HOXA5, PPP2R3A, PTEN and TNFSF8). In experiment 2, embryos were treated at Day 5 with control or CSF2 and blastocysts were collected at Day 7 for immunolabeling to determine the number of inner cell mass (ICM) and trophectoderm (TE) cells. CSF2 increased the percent of putative zygotes that became blastocysts for females, but did not affect the development of males. There was no effect of CSF2 or interaction of CSF2 with sex on the total number of blastomeres in blastocysts or in the number of inner cell mass or trophectoderm cells. In conclusion, CSF2 exerted divergent responses on gene expression and development of female and male embryos. These results are evidence of sexually dimorphic responses of the preimplantation embryo to this embryokine. 653 $aCSF2 653 $aEmbryokine 700 1 $aHANSEN, P. J. 773 $tReproduction$gn. 152, p. 645-654, 2016.
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