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| Registros recuperados : 136 | |
| 5. |  | CARDOSO, A. N.; SIQUEIRA, F. G. de; MENDONCA, S. LCA of a biologically detoxified cottonseed cake to be used for monogastric nutrition. In: GIANETTI, B. F.; ALMEIDA, C. M. V. B.; AGOSTINHO, F. (ed.). In: INTERNATIONAL WORKSHOP ON ADVANCES IN CLEANER PRODUCTION, 9., 2020, Melbourne. Melbourne, 2020. Online Workshop.| Biblioteca(s): Embrapa Agroenergia. |
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| 12. | | MARQUES, H. P.; SIQUEIRA, F. G. de; LEAO, A. P.; SOUZA JUNIOR, M. T. Otimização de métodos de extração de DNA genômico dos basidiomicetos Panus lecomtei CC40 e Pleurotus pulmonarius EF88. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 4.,2017, Brasília, DF. Anais... Brasília, DF: Embrapa, 2017. p. 33-38.| Biblioteca(s): Embrapa Agroenergia. |
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| 14. | | HARTMANN, C.; FONTANA, R. C.; SIQUEIRA, F. G. de; CAMASSOLA, M. Produção de enzimas lignolíticas por diferentes espécies de basidiomicetos em cultivo em estado sólido. In: CONGRESSO BRASILEIRO DE ENGENHARIA QUÍMICA - COBEC, 21.; Encontro Brasileiro sobre o Ensino de Engenharia Química - ENBEQ, , 2016, Fortaleza, CE. [Anais ...]. São Paulo: Associação Brasileira de Engenharia Química, 2016.| Biblioteca(s): Embrapa Agroenergia. |
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| 15. | | SANTANA, T. D. D.; MENDES, T. D.; MAGALHÃES, P. O.; MENDONCA, S.; SIQUEIRA, F. G. de. Proteases e fitases de macrofungos cultivados em meio submerso com torta de pinhão-manso ou caroço de algodão como fonte de carbono. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 2., 2015, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2015. p. 103-105| Biblioteca(s): Embrapa Agroenergia. |
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| 20. | | HELM, C. V.; ALCANTARA, L. M.; BRASIL, B. dos S. A. F.; LAVIOLA, B. G.; SIQUEIRA, F. G. de. Biomass growth of the white-rot fungi Auricularia auricula-judae on cheese whey. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 36., 2014, Clearwater Beach, FL. Abstracts. Fairfax: Society for Industrial Microbiology and Biotechnology, 2014. p. 90.| Biblioteca(s): Embrapa Agroenergia; Embrapa Florestas. |
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| Registros recuperados : 136 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
02/08/2023 |
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Data da última atualização: |
26/10/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
GOMES, T. G.; FONSECA, F. C. de A.; ALVES, G. S. C.; SIQUEIRA, F. G. de; MILLER, R. N. G. |
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Afiliação: |
TAÍSA GODOY GOMES, Universidade de Brasília; FERNANDO CAMPOS DE ASSIS FONSECA, Universidade de Brasília; GABRIEL SERGIO COSTA ALVES, Universidade de Brasília; FELIX GONCALVES DE SIQUEIRA, CNPAE; ROBERT NEIL GERARD MILLER, Universidade de Brasília. |
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Título: |
Development of reference genes for RT-qPCR analysis of gene expression in Pleurotus pulmonarius for biotechnological applications. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Scientific Reports, v. 13, n. 12296, 2023. |
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DOI: |
https://doi.org/10.1038/s41598-023-39115-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Jatropha curcas is an oilseed crop with biorefinery applications. Whilst cake generated following oil extraction offers potential as a protein source for animal feed, inactivation of toxic phorbol esters present in the material is necessary. Pleurotus pulmonarius is a detoxifying agent for jatropha cake with additional potential as animal feed, edible mushroom and for enzyme production. For the characterization of fungal genes involved in phorbol ester degradation, together with other industrial applications, reverse transcription-quantitative PCR (RT-qPCR) is a tool that enables accurate quantification of gene expression. For this, reliable analysis requires reference genes for normalization of mRNA levels validated under conditions employed for target genes. The stability of potential reference genes ?-TUB, ACTIN, GAPDH, PHOS, EF1?, TRPHO, LAC, MNP3, MYP and VP were evaluated following growth of P. pulmonarius on toxic, non-toxic jatropha cake and a combined treatment, respectively. NormFinder and geNorm algorithms for expression stability analysis identified PHOS, EF1? and MNP3 as appropriate for normalizing gene expression. Reference gene combinations contrasting in ranking were compared following normalization of relative expression of the CHU_2040 gene, encoding an esterase enzyme potentially involved in phorbol ester degradation. The reference genes for P. pulmonarius will facilitate the elucidation of mechanisms involved in detoxification of phorbol esters as well as analysis of target genes for application in biorefinery models. MenosJatropha curcas is an oilseed crop with biorefinery applications. Whilst cake generated following oil extraction offers potential as a protein source for animal feed, inactivation of toxic phorbol esters present in the material is necessary. Pleurotus pulmonarius is a detoxifying agent for jatropha cake with additional potential as animal feed, edible mushroom and for enzyme production. For the characterization of fungal genes involved in phorbol ester degradation, together with other industrial applications, reverse transcription-quantitative PCR (RT-qPCR) is a tool that enables accurate quantification of gene expression. For this, reliable analysis requires reference genes for normalization of mRNA levels validated under conditions employed for target genes. The stability of potential reference genes ?-TUB, ACTIN, GAPDH, PHOS, EF1?, TRPHO, LAC, MNP3, MYP and VP were evaluated following growth of P. pulmonarius on toxic, non-toxic jatropha cake and a combined treatment, respectively. NormFinder and geNorm algorithms for expression stability analysis identified PHOS, EF1? and MNP3 as appropriate for normalizing gene expression. Reference gene combinations contrasting in ranking were compared following normalization of relative expression of the CHU_2040 gene, encoding an esterase enzyme potentially involved in phorbol ester degradation. The reference genes for P. pulmonarius will facilitate the elucidation of mechanisms involved in detoxification of phorbol esters as well as... Mostrar Tudo |
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Thesagro: |
Jatropha Curcas; Refinaria. |
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Thesaurus NAL: |
Oilseed crops. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1155580/1/Development-of-reference.pdf
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Marc: |
LEADER 02270naa a2200217 a 4500
001 2155580
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024 7 $ahttps://doi.org/10.1038/s41598-023-39115-4$2DOI
100 1 $aGOMES, T. G.
245 $aDevelopment of reference genes for RT-qPCR analysis of gene expression in Pleurotus pulmonarius for biotechnological applications.$h[electronic resource]
260 $c2023
520 $aJatropha curcas is an oilseed crop with biorefinery applications. Whilst cake generated following oil extraction offers potential as a protein source for animal feed, inactivation of toxic phorbol esters present in the material is necessary. Pleurotus pulmonarius is a detoxifying agent for jatropha cake with additional potential as animal feed, edible mushroom and for enzyme production. For the characterization of fungal genes involved in phorbol ester degradation, together with other industrial applications, reverse transcription-quantitative PCR (RT-qPCR) is a tool that enables accurate quantification of gene expression. For this, reliable analysis requires reference genes for normalization of mRNA levels validated under conditions employed for target genes. The stability of potential reference genes ?-TUB, ACTIN, GAPDH, PHOS, EF1?, TRPHO, LAC, MNP3, MYP and VP were evaluated following growth of P. pulmonarius on toxic, non-toxic jatropha cake and a combined treatment, respectively. NormFinder and geNorm algorithms for expression stability analysis identified PHOS, EF1? and MNP3 as appropriate for normalizing gene expression. Reference gene combinations contrasting in ranking were compared following normalization of relative expression of the CHU_2040 gene, encoding an esterase enzyme potentially involved in phorbol ester degradation. The reference genes for P. pulmonarius will facilitate the elucidation of mechanisms involved in detoxification of phorbol esters as well as analysis of target genes for application in biorefinery models.
650 $aOilseed crops
650 $aJatropha Curcas
650 $aRefinaria
700 1 $aFONSECA, F. C. de A.
700 1 $aALVES, G. S. C.
700 1 $aSIQUEIRA, F. G. de
700 1 $aMILLER, R. N. G.
773 $tScientific Reports$gv. 13, n. 12296, 2023.
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Embrapa Agroenergia (CNPAE) |
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