Data/hora: 11/06/2024 23:36:24

Biblioteca(s): 	Embrapa Caprinos e Ovinos.	
Tipo da produção científica: 	Artigo em Periódico Indexado	
Circulação/Nível: 	Internacional - A	
Data corrente: 	26/06/2012	
Data da última atualização: 	08/09/2017	
Autoria: 	KESKINTEPE, L.; SIMPLICIO, A. A.; BRACKETT, B. G.	
Afiliação: 	Augusta Repro. Biology Associates, 812 Chafee Ave., United States; Aurino Alves Simplício, CNPC; Dept. of Physiol. and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, United States.	
Título: 	Caprine blastocyst development after in vitro fertilization with spermatozoa frozen in different extenders.	
Ano de publicação: 	1998	
Fonte/Imprenta: 	Theriogenology, New York, v. 49, n. 7, p. 1265-1274, May, 1998.	
DOI: 	10.1016/S0093-691X(98)00074-0	
Idioma: 	Inglês	
Conteúdo: 	Abstract: The feasibility of using frozen-thawed semen in caprine IVF outside the breeding season was investigated. Electroejaculated spermatozoa from a Nubian buck were washed twice and then frozen in skim milk- or in egg yolk-based extenders. Goat oocytes were matured and inseminated by frozen-thawed spermatozoa selected by swim-up. In vitro fertilization was performed in a modified defined medium (mDM), altered experimentally, for 24 h. Embryos were cultured in 50 ?L of c-SOF+NEA for 9 d. The percentages of oocytes exposed to heparin-capacitated spermatozoa, (previously cryopreserved in skim milk- based extender) that cleaved, reached morula, blastocyst and expanded blastocyst stages were 82.8, 57.1, 35.7 and 30.0%, respectively. Without heparin treatment the rates for cleavage, morula, blastocyst and expanded blastocyst stages were 44.3, 31.4, 18.6 and 8.6%, respectively. Therefore, heparin treatment was included in sperm capacitation. Use of spermatozoa with BSA in the IVF medium yielded no cleavage. Although extenders containing 8 to 20% egg yolk enabled good sperm motility after cryopreservation, in vitro fertilizing ability was compromised under our conditions. By contrast, semen commercially processed in season in an egg yolk-based diluent remained effective for IVF. The highest proportion of blastocysts resulted from the use of spermatozoa diluted in a skim milk extender, heparin capacitation, and insemination in medium containing lamb serum.	
Palavras-Chave: 	Animal embryos; Blastocisto; Extenders; Ovary; Sperm cryopreservation; Sperm preservation.	
Thesagro: 	Caprino; Criopreservação; Espermatozóide; Inseminação artificial; Reprodução animal; Sêmen.	
Thesaurus Nal: 	Animal breeding; Blastocystis; Goats; In vitro fertilization; Reproduction.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Caprinos e Ovinos (CNPC)	
Exemplares: 	CNPC - (25680-1) - UPC - AP - DD -  -  -  - DIS