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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
20/05/1993 |
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Data da última atualização: |
20/05/1993 |
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Autoria: |
GARNSEY, S. M.; BAR-JOSEPH, M.; LEE, R. F. |
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Afiliação: |
U.S. Department of Agriculture, Agricultural Research Service, Horticultural Research Laboratory, Orlando, FL 32803, USA. |
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Título: |
Applications of serological indexing to develop control strategies for citrus tristeza virus. |
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Ano de publicação: |
1981 |
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Fonte/Imprenta: |
Tokyo, Japan: International Society of Citricultural, 1981. |
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Volume: |
v.1 |
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Páginas: |
p.448-452 |
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Idioma: |
Inglês |
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Conteúdo: |
The development of antisera specific to citrus tristeza virus (CTV) and use of CTV antisera with sensitive, rapid detection technique such as enzyme linked immunosorbent assay (ELISA) has greatly facilitated CTV indexing. These serological indexing techniques have created new opportunities to control CTV and new opportunities for research. In areas where CTV is not yet widespread, such as Israel, parts of the U.S., and parts of the Mediterranean Basin, serology has offered new hope for eradication or long-term suppression of CTV. The ELISA technique has been used to test more than 600,000 trees for CTV in a countrywide survey in Israel. New loci of infection, some symptomless, were located rapidly. Because results can be obtained within hours after sampling, diseased trees can be removed rapidly to reduce secondary spread. Serology has been used in Florida to test budwood trees for CTV and to monitor natural spread in field plantings. Serological indexing is adpted for the rapid, repeated testing of many samples in epidemiology studies, and detection of CTV in aphids by ELISA has been reported. ELISA is being used to identify CTV-resistant hybrids in crosses between Citrus and Poncirus trifoliata (L.) Raf. and other hybrids, and this rapid, quantitative measurrement of virus titer has improved screening. Serology is also being used in research on mild strain cross protection to verify presence of symptomless, mild isolates in inoculated, protected trees. However, mild and severe CTV strains... MenosThe development of antisera specific to citrus tristeza virus (CTV) and use of CTV antisera with sensitive, rapid detection technique such as enzyme linked immunosorbent assay (ELISA) has greatly facilitated CTV indexing. These serological indexing techniques have created new opportunities to control CTV and new opportunities for research. In areas where CTV is not yet widespread, such as Israel, parts of the U.S., and parts of the Mediterranean Basin, serology has offered new hope for eradication or long-term suppression of CTV. The ELISA technique has been used to test more than 600,000 trees for CTV in a countrywide survey in Israel. New loci of infection, some symptomless, were located rapidly. Because results can be obtained within hours after sampling, diseased trees can be removed rapidly to reduce secondary spread. Serology has been used in Florida to test budwood trees for CTV and to monitor natural spread in field plantings. Serological indexing is adpted for the rapid, repeated testing of many samples in epidemiology studies, and detection of CTV in aphids by ELISA has been reported. ELISA is being used to identify CTV-resistant hybrids in crosses between Citrus and Poncirus trifoliata (L.) Raf. and other hybrids, and this rapid, quantitative measurrement of virus titer has improved screening. Serology is also being used in research on mild strain cross protection to verify presence of symptomless, mild isolates in inoculated, protected trees. However, mild and se... Mostrar Tudo |
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Palavras-Chave: |
Cross protection; Enzyme linked immunosorbent assay (ELISA); Host resistance; Immunodiffusion; Immunofluorescence; Radio immunosorbent assay; serologically specific electron microscopy (SSEM). |
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Thesaurus Nal: |
epidemiology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02330naa a2200265 a 4500
001 1644018
005 1993-05-20
008 1981 bl uuuu u00u1 u #d
100 1 $aGARNSEY, S. M.
245 $aApplications of serological indexing to develop control strategies for citrus tristeza virus.
260 $c1981
300 $ap.448-452 v.1
490 $vv.1
520 $aThe development of antisera specific to citrus tristeza virus (CTV) and use of CTV antisera with sensitive, rapid detection technique such as enzyme linked immunosorbent assay (ELISA) has greatly facilitated CTV indexing. These serological indexing techniques have created new opportunities to control CTV and new opportunities for research. In areas where CTV is not yet widespread, such as Israel, parts of the U.S., and parts of the Mediterranean Basin, serology has offered new hope for eradication or long-term suppression of CTV. The ELISA technique has been used to test more than 600,000 trees for CTV in a countrywide survey in Israel. New loci of infection, some symptomless, were located rapidly. Because results can be obtained within hours after sampling, diseased trees can be removed rapidly to reduce secondary spread. Serology has been used in Florida to test budwood trees for CTV and to monitor natural spread in field plantings. Serological indexing is adpted for the rapid, repeated testing of many samples in epidemiology studies, and detection of CTV in aphids by ELISA has been reported. ELISA is being used to identify CTV-resistant hybrids in crosses between Citrus and Poncirus trifoliata (L.) Raf. and other hybrids, and this rapid, quantitative measurrement of virus titer has improved screening. Serology is also being used in research on mild strain cross protection to verify presence of symptomless, mild isolates in inoculated, protected trees. However, mild and severe CTV strains...
650 $aepidemiology
653 $aCross protection
653 $aEnzyme linked immunosorbent assay (ELISA)
653 $aHost resistance
653 $aImmunodiffusion
653 $aImmunofluorescence
653 $aRadio immunosorbent assay
653 $aserologically specific electron microscopy (SSEM)
700 1 $aBAR-JOSEPH, M.
700 1 $aLEE, R. F.
773 $tTokyo, Japan: International Society of Citricultural, 1981.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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