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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
23/03/2005 |
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Data da última atualização: |
23/03/2005 |
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Autoria: |
GONZALEZ-BULNES, A.; GARCIA-GARCIA, R. M.; SANTIAGO-MORENO, J. |
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Título: |
Embryo viability decreases during early development in the genital tract of superovulated ewes. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Theriogenology, v. 53, n. 1, p. 272, 2000. |
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Idioma: |
Inglês |
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Notas: |
Edição de proceedings Annual Conference of the International Embryo Transfer Society, Maastricht, The Netherlands, jan. 2000. |
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Conteúdo: |
Ovarian responses to exogenous FSH in sheep is affected by an inherent variability which can diminish the final number of transferable embryos collected. We have focused this study on the possible causes related to early embryo development, by assessing differences in viability of embryos recovered either from the oviduct or the uterus. Estrus was synchronized in 39 Manchega ewes using 40 mg FGA sponges inserted on Day 0 and exchanged with a fresh one on Days 7 to 14. A single dose of 125 mg cloprostenol (im) was given coincidentally with the first FSH dose. The superovulatory treatment consisted of 8 decreasing doses (1.5 mL x 3; 1.25 mL x 2 and I mL x 3) of OvagenTM (ICP, Auckland, NZ) given twice daily (im) from 60 h before to 24 h after sponge removal. Mating was performed at 24, 36 and 48 h after progestagen sponge withdrawal. Ovulatory responses were determined by laparoscopy, and embryo quality was evaluated by standard morphological criteria just after surgical recovery from the uterine horns 7 days after sponge removal (Group 1: 1.8 ewes), or assessing in vitro development to expanding or hatching blastocysts in co-culture on oviductal cell monolayers when embryos were obtained from oviductal flushes on Day 4 (Group 2: 21 ewes). Possible differences in the numbers of corpora lutea (CL) and number of recovered, viable and degenerate embryos were assessed by ANOVA Comparisons were made in the recovery efficiency (total embryos recovered from total number of CL) viability (number of viable embryos from the total recovered) and degeneration rate (number of degenerate embryos from the total recovered). There were no differences in ovulation rates between Group 1 and Group 2 (11.7 +1.7 vs 11.3+1.2 CL; P=0.989). However, the number of viable embryos obtained from the uterine horns tended to be lower than those collected from the oviducts (5.1+1.3 vs 6.5+1.0), resulting , in a significantly higher viability rate in embryos from oviduct retrieval (72.5 vs 58.6%, P<0.05). On the other band, the numbers of degenerate embryos were significantly higher in uterine recoveries (32.5 vs 5.3%, P<0.005). These results give new evidence supporting the hypothesis that the quality of embryos obtained from superovulated females decreases during early development, with increasing length of time in the genital tract of donor. However. studies are necessary to determine whether tbis fact is related to alterations in the endocrine patterns and/or perturbations in the oviductal/uterine environment of the donor ewe as a result of the exogenous FSH treatment. MenosOvarian responses to exogenous FSH in sheep is affected by an inherent variability which can diminish the final number of transferable embryos collected. We have focused this study on the possible causes related to early embryo development, by assessing differences in viability of embryos recovered either from the oviduct or the uterus. Estrus was synchronized in 39 Manchega ewes using 40 mg FGA sponges inserted on Day 0 and exchanged with a fresh one on Days 7 to 14. A single dose of 125 mg cloprostenol (im) was given coincidentally with the first FSH dose. The superovulatory treatment consisted of 8 decreasing doses (1.5 mL x 3; 1.25 mL x 2 and I mL x 3) of OvagenTM (ICP, Auckland, NZ) given twice daily (im) from 60 h before to 24 h after sponge removal. Mating was performed at 24, 36 and 48 h after progestagen sponge withdrawal. Ovulatory responses were determined by laparoscopy, and embryo quality was evaluated by standard morphological criteria just after surgical recovery from the uterine horns 7 days after sponge removal (Group 1: 1.8 ewes), or assessing in vitro development to expanding or hatching blastocysts in co-culture on oviductal cell monolayers when embryos were obtained from oviductal flushes on Day 4 (Group 2: 21 ewes). Possible differences in the numbers of corpora lutea (CL) and number of recovered, viable and degenerate embryos were assessed by ANOVA Comparisons were made in the recovery efficiency (total embryos recovered from total number of CL) viabi... Mostrar Tudo |
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Palavras-Chave: |
FSH; Regressão prematura; Superovulaçãio; Trato genital. |
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Thesagro: |
Ovelha; Ovino; Reprodução Animal. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03374naa a2200241 a 4500
001 1531021
005 2005-03-23
008 2000 bl uuuu u00u1 u #d
100 1 $aGONZALEZ-BULNES, A.
245 $aEmbryo viability decreases during early development in the genital tract of superovulated ewes.
260 $c2000
500 $aEdição de proceedings Annual Conference of the International Embryo Transfer Society, Maastricht, The Netherlands, jan. 2000.
520 $aOvarian responses to exogenous FSH in sheep is affected by an inherent variability which can diminish the final number of transferable embryos collected. We have focused this study on the possible causes related to early embryo development, by assessing differences in viability of embryos recovered either from the oviduct or the uterus. Estrus was synchronized in 39 Manchega ewes using 40 mg FGA sponges inserted on Day 0 and exchanged with a fresh one on Days 7 to 14. A single dose of 125 mg cloprostenol (im) was given coincidentally with the first FSH dose. The superovulatory treatment consisted of 8 decreasing doses (1.5 mL x 3; 1.25 mL x 2 and I mL x 3) of OvagenTM (ICP, Auckland, NZ) given twice daily (im) from 60 h before to 24 h after sponge removal. Mating was performed at 24, 36 and 48 h after progestagen sponge withdrawal. Ovulatory responses were determined by laparoscopy, and embryo quality was evaluated by standard morphological criteria just after surgical recovery from the uterine horns 7 days after sponge removal (Group 1: 1.8 ewes), or assessing in vitro development to expanding or hatching blastocysts in co-culture on oviductal cell monolayers when embryos were obtained from oviductal flushes on Day 4 (Group 2: 21 ewes). Possible differences in the numbers of corpora lutea (CL) and number of recovered, viable and degenerate embryos were assessed by ANOVA Comparisons were made in the recovery efficiency (total embryos recovered from total number of CL) viability (number of viable embryos from the total recovered) and degeneration rate (number of degenerate embryos from the total recovered). There were no differences in ovulation rates between Group 1 and Group 2 (11.7 +1.7 vs 11.3+1.2 CL; P=0.989). However, the number of viable embryos obtained from the uterine horns tended to be lower than those collected from the oviducts (5.1+1.3 vs 6.5+1.0), resulting , in a significantly higher viability rate in embryos from oviduct retrieval (72.5 vs 58.6%, P<0.05). On the other band, the numbers of degenerate embryos were significantly higher in uterine recoveries (32.5 vs 5.3%, P<0.005). These results give new evidence supporting the hypothesis that the quality of embryos obtained from superovulated females decreases during early development, with increasing length of time in the genital tract of donor. However. studies are necessary to determine whether tbis fact is related to alterations in the endocrine patterns and/or perturbations in the oviductal/uterine environment of the donor ewe as a result of the exogenous FSH treatment.
650 $aOvelha
650 $aOvino
650 $aReprodução Animal
653 $aFSH
653 $aRegressão prematura
653 $aSuperovulaçãio
653 $aTrato genital
700 1 $aGARCIA-GARCIA, R. M.
700 1 $aSANTIAGO-MORENO, J.
773 $tTheriogenology$gv. 53, n. 1, p. 272, 2000.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
07/12/2001 |
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Data da última atualização: |
07/12/2001 |
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Autoria: |
GIORDANO, L. de B.; SILVA-LOBO, V. L. da; SANTANA, F. M.; BOITEUX, L. S. |
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Afiliação: |
EMBRAPA-CNPH, Brasília, DF. |
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Título: |
Herança da resistência a geminivirus em tomateiro. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Horticultura Brasileira, Brasília, DF, v. 19, n. 2, jul. 2001. |
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Páginas: |
não paginado. |
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Descrição Física: |
CD-ROM. |
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Idioma: |
Português |
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Notas: |
Trabalho apresentado no 41° Congresso Brasileiro de Olericultura, 2001. |
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Thesagro: |
Doença; Lycopersicon Esculentum; Resistência; Tomate; Vírus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00696naa a2200229 a 4500
001 1770938
005 2001-12-07
008 2001 bl uuuu u00u1 u #d
100 1 $aGIORDANO, L. de B.
245 $aHerança da resistência a geminivirus em tomateiro.
260 $c2001
300 $anão paginado. $cCD-ROM.
500 $aTrabalho apresentado no 41° Congresso Brasileiro de Olericultura, 2001.
650 $aDoença
650 $aLycopersicon Esculentum
650 $aResistência
650 $aTomate
650 $aVírus
700 1 $aSILVA-LOBO, V. L. da
700 1 $aSANTANA, F. M.
700 1 $aBOITEUX, L. S.
773 $tHorticultura Brasileira, Brasília, DF$gv. 19, n. 2, jul. 2001.
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