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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
03/11/2011 |
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Data da última atualização: |
30/06/2015 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SOUZA, M. M.; BRESSANI, F. A.; TIZIOTO, P. C.; MALAGÓ-JR, W.; SIQUEIRA, F.; ROSA, A. do N.; SILVA, L. O. C. da; TORRES JUNIOR, R. A. de A.; MEDEIROS, S. R. de; TULLIO, R. R.; ALENCAR, M. M. de; NICIURA, S. C. M.; REGITANO, L. C. de A. |
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Afiliação: |
PÓS-GRADUAÇÃO UFSCar/SÃO CARLOS, SP; FLAVIA ALINE BRESSANI DONATONI, CPPSE; PÓS-GRADUAÇÃO UFSCar/SÃO CARLOS, SP; WILSON MALAGÓ JUNIOR, PÓS-GRADUAÇÃO UFSCar/SÃO CARLOS, SP; FABIANE SIQUEIRA, CNPGC; ANTONIO DO NASCIMENTO ROSA, CNPGC; LUIZ OTAVIO CAMPOS DA SILVA, CNPGC; ROBERTO AUGUSTO DE A TORRES JUNIOR, CNPGC; SERGIO RAPOSO DE MEDEIROS, CNPGC; RYMER RAMIZ TULLIO, CPPSE; MAURICIO MELLO DE ALENCAR, CPPSE; SIMONE CRISTINA MEO NICIURA, CPPSE; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
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Título: |
Searching for snp in capn1 gene and its association with shear force in nellore cattle. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Archivos Latinoamericano de Producion Animal, v. 19, (supl. 1), p. 233, 2011 |
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Idioma: |
Português |
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Palavras-Chave: |
CAPN1 gene; Shear force; SNP. |
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Thesaurus Nal: |
beef cattle; Nellore. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 00884nam a2200301 a 4500
001 1904836
005 2015-06-30
008 2011 bl uuuu u00u1 u #d
100 1 $aSOUZA, M. M.
245 $aSearching for snp in capn1 gene and its association with shear force in nellore cattle.
260 $aArchivos Latinoamericano de Producion Animal, v. 19, (supl. 1), p. 233, 2011$c2011
650 $abeef cattle
650 $aNellore
653 $aCAPN1 gene
653 $aShear force
653 $aSNP
700 1 $aBRESSANI, F. A.
700 1 $aTIZIOTO, P. C.
700 1 $aMALAGÓ-JR, W.
700 1 $aSIQUEIRA, F.
700 1 $aROSA, A. do N.
700 1 $aSILVA, L. O. C. da
700 1 $aTORRES JUNIOR, R. A. de A.
700 1 $aMEDEIROS, S. R. de
700 1 $aTULLIO, R. R.
700 1 $aALENCAR, M. M. de
700 1 $aNICIURA, S. C. M.
700 1 $aREGITANO, L. C. de A.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
25/06/2013 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ARAUJO, A. C. G. de; MORGANTE, C. V.; GUIMARAES, P. M.; SILVA JUNIOR, O. B. da; ROBERTS, P. A.; BERTIOLI, S. C. de M. L.; BERTIOLI, D.; BRASILEIRO, A. C. M. |
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Afiliação: |
ANA CLAUDIA GUERRA DE ARAUJO, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; PATRICIA MESSEMBERG GUIMARAES, Cenargen; ORZENIL BONFIM DA SILVA JUNIOR, CENARGEN; SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; UnB; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN. |
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Título: |
A survey of differentially expressed genes in resistant wild arachis during nematode infection. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: SBG, 2013. |
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Páginas: |
p. 58. |
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Idioma: |
Inglês |
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Conteúdo: |
The plant parasitic root-knot nematode evolved sophisticated strategies for exploiting plants and causes devastating yield penalties worldwide. The main control strategy of this parasite is the use of nematicides, which are contaminants for groundwater systems and toxic for human health. Until now, relatively little is known about crucial events related to the establishment and eproduction of these nematodes in peanut (Arachis hypogaea), and even less about the resistance mechanisms on this plant host. Wild relatives of crop species are an important source of genes for resistance to biotic stresses and, in particular, the wild peanut relative Arachis stenosperma shows high levels of resistance to Meloidogyne arenaria and other parasites. A genome-wide overview of gene expression during this plant nematode interaction at different time points of interaction [3, 6 and 9 days after inoculation (DAI)] was conducted using Illumina Hi-Seq 2000. For that, eight cDNA libraries were constructed using total RNA from inoculated and non inoculated roots of A. stenorpema plants, in two biological replicates. The total sequence data produced 38,241.633 reads with an average sequence size of 200 bp that were assembled into 44,133 contigs. Ten clusters were further identified by K-means clustering (MEV program) based on their expression patterns and in silico analysis showed a comparable number of genes that were significantly up- and down-regulated during the resistance response. Sequences were GO categorized and genes related to diverse classes of function and process in the cell were identified. Based on these analyses, defence-related genes, transcription factors, components of hormonal signaling pathways and genes involved on cell wall metabolism were further selected for qRT-PCR analysis to validate their expression profile. A number of genes showed comparable expression pattern on both qRT-PCR and in silico analysis. The transcriptome data obtained in this study is a helpful resource to identify genes regulated during early response of resistant Arachis species to M. arenaria which will help the understanding of the resistance mechanisms and the selection of candidate genes to be validated via plant transformation. A number of SSR and SNP markers will also be developed from these data providing new tools for peanut breeding program. MenosThe plant parasitic root-knot nematode evolved sophisticated strategies for exploiting plants and causes devastating yield penalties worldwide. The main control strategy of this parasite is the use of nematicides, which are contaminants for groundwater systems and toxic for human health. Until now, relatively little is known about crucial events related to the establishment and eproduction of these nematodes in peanut (Arachis hypogaea), and even less about the resistance mechanisms on this plant host. Wild relatives of crop species are an important source of genes for resistance to biotic stresses and, in particular, the wild peanut relative Arachis stenosperma shows high levels of resistance to Meloidogyne arenaria and other parasites. A genome-wide overview of gene expression during this plant nematode interaction at different time points of interaction [3, 6 and 9 days after inoculation (DAI)] was conducted using Illumina Hi-Seq 2000. For that, eight cDNA libraries were constructed using total RNA from inoculated and non inoculated roots of A. stenorpema plants, in two biological replicates. The total sequence data produced 38,241.633 reads with an average sequence size of 200 bp that were assembled into 44,133 contigs. Ten clusters were further identified by K-means clustering (MEV program) based on their expression patterns and in silico analysis showed a comparable number of genes that were significantly up- and down-regulated during the resistance response. Sequences... Mostrar Tudo |
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Palavras-Chave: |
Nematóide das galhas; Root-knot nematode. |
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Thesagro: |
Amendoim; Doença. |
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Thesaurus NAL: |
Arachis; Meloidogyne. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/84814/1/Carolina-2013-1.pdf
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Marc: |
LEADER 03257nam a2200277 a 4500
001 1960580
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
100 1 $aARAUJO, A. C. G. de
245 $aA survey of differentially expressed genes in resistant wild arachis during nematode infection.$h[electronic resource]
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: SBG$c2013
300 $ap. 58.
520 $aThe plant parasitic root-knot nematode evolved sophisticated strategies for exploiting plants and causes devastating yield penalties worldwide. The main control strategy of this parasite is the use of nematicides, which are contaminants for groundwater systems and toxic for human health. Until now, relatively little is known about crucial events related to the establishment and eproduction of these nematodes in peanut (Arachis hypogaea), and even less about the resistance mechanisms on this plant host. Wild relatives of crop species are an important source of genes for resistance to biotic stresses and, in particular, the wild peanut relative Arachis stenosperma shows high levels of resistance to Meloidogyne arenaria and other parasites. A genome-wide overview of gene expression during this plant nematode interaction at different time points of interaction [3, 6 and 9 days after inoculation (DAI)] was conducted using Illumina Hi-Seq 2000. For that, eight cDNA libraries were constructed using total RNA from inoculated and non inoculated roots of A. stenorpema plants, in two biological replicates. The total sequence data produced 38,241.633 reads with an average sequence size of 200 bp that were assembled into 44,133 contigs. Ten clusters were further identified by K-means clustering (MEV program) based on their expression patterns and in silico analysis showed a comparable number of genes that were significantly up- and down-regulated during the resistance response. Sequences were GO categorized and genes related to diverse classes of function and process in the cell were identified. Based on these analyses, defence-related genes, transcription factors, components of hormonal signaling pathways and genes involved on cell wall metabolism were further selected for qRT-PCR analysis to validate their expression profile. A number of genes showed comparable expression pattern on both qRT-PCR and in silico analysis. The transcriptome data obtained in this study is a helpful resource to identify genes regulated during early response of resistant Arachis species to M. arenaria which will help the understanding of the resistance mechanisms and the selection of candidate genes to be validated via plant transformation. A number of SSR and SNP markers will also be developed from these data providing new tools for peanut breeding program.
650 $aArachis
650 $aMeloidogyne
650 $aAmendoim
650 $aDoença
653 $aNematóide das galhas
653 $aRoot-knot nematode
700 1 $aMORGANTE, C. V.
700 1 $aGUIMARAES, P. M.
700 1 $aSILVA JUNIOR, O. B. da
700 1 $aROBERTS, P. A.
700 1 $aBERTIOLI, S. C. de M. L.
700 1 $aBERTIOLI, D.
700 1 $aBRASILEIRO, A. C. M.
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