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| Registros recuperados : 110 | |
| 41. |  | SILVA JUNIOR, O. B. da; RESENDE, L. V.; VIDAL, R. F.; BORDALLO, P. N.; SILVA, P. I. T.; MELO, D. S.; TOGAWA, R. C.; GRATTAPAGLIA, D. A chromosome-scale assembly of the cashew tree genome: leveraging the combined power of PacBio, DNA-Chromatin Proximity Ligation and SNP linkage maps. In: PLANT AND ANIMAL GENOME CONFERENCE, 25., 2017, San Diego. [Abstracts...]. San Diego, CA: [s.n.], 2017. W354.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 42. | | SOBREIRO, M. B.; VIEIRA, L. D.; NUNES, R.; NOVAES, E.; COISSAC, E.; SILVA JUNIOR, O. B.; GRATTAPAGLIA, D.; COLLEVATTI, R. G. Chloroplast genome assembly of Handroanthus impetiginosus: comparative analysis and molecular evolution in Bignoniaceae. Planta, v. 252, 91, 2020.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 43. | | RESENDE, R. T.; PIEPHO,H.-P.; ROSA, G. J. M.; SILVA JUNIOR, O. B. da; SILVA, F. F. e; RESENDE, M. D. V. de; GRATTAPAGLIA, D. Enviromics in breeding: applications and perspectives on envirotypic-assisted selection. Theoretical and Applied Genetics, v. 134, n. 1, 2021. p. 95-112.| Biblioteca(s): Embrapa Café; Embrapa Recursos Genéticos e Biotecnologia. |
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| 44. | | SILVA JUNIOR, O. B. da; TOGAWA, R. C.; NOVAES, E.; FARIA, D. A. de; PAPPAS JUNIOR, G. J.; MATIAS, K.; GRATTAPAGLIA, D. Assessment of predicted assay functionality of Eucalyptus SNP derived from large interspecific collections of ESTs. In: IUFRO TREE BIOTECHNOLOGY CONFERENCE, 2009, Whislter. Abstracts. [S.l.]: IUFRO, 2009. p-52| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 45. | | LIMA, B. M.; SILVA JUNIOR, O. B. da; FARIA, D. A.; MAMANI, E. M. C.; PAPPAS JUNIOR, G. J.; GRATTAPAGLIA, D. Assessment of SNPs for linkage mapping in Eucalyptus: construction of a consensus SNP/microsatellite map from two unrelated pedigrees. BMC Proceedings, v. 5, suppl, P31, 2012. (Open access) Edição do Proceedings IUFRO Tree Biotechnology Conference, Arraial d?Ajuda, 2011.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 46. | | RESENDE, R. T.; RESENDE, M. D. V.; SILVA, F. F.; AZEVEDO, C. F.; TAKAHASHI, E. K.; SILVA JUNIOR, O. B.; GRATTAPAGLIA, D. Assessing the expected response to genomic selection of individuals and families in Eucalyptus breeding with an additive-dominant model. Heredity, v. 119, p. 245-255, 2017.| Biblioteca(s): Embrapa Florestas; Embrapa Recursos Genéticos e Biotecnologia. |
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| 47. | | PESSOA FILHO, M. A. C. de P.; SILVA, P. I. T.; RESENDE, L. V.; VIEIRA, E. A.; FALEIRO, F. G.; GRATTAPAGLIA, D.; SILVA JUNIOR, O. B. da. Application of the Axiom 3K SNP genotyping array in cassava breeding and genetics. In: PLANT AND ANIMAL GENOME CONFERENCE, 26., 2018, San Diego. Proceedings... Jersey City, NJ: Scherago International, 2018. PAG XXVI| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 48. | | PESSOA FILHO, M. A. C. de P.; SILVA, P. I. T.; RESENDE, L. V.; VIEIRA, E. A.; FALEIRO, F. G.; GRATTAPAGLIA, D.; SILVA JUNIOR, O. B. da. Application of the Axiom 3K SNP genotyping array in cassava breeding and genetics. In: PLANT AND ANIMAL GENOME CONFERENCE, 26., 2018, San Diego. Proceedings... Jersey City, NJ: Scherago International, 2018. PAG XXVI| Biblioteca(s): Embrapa Cerrados. |
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| 49. | | PESSOA FILHO, M. A. C. de P.; SOUZA SOBRINHO, F. de; FRAGOSO, R. da R.; SILVA JUNIOR, O. B. da; FERREIRA, M. E. A draft genome assembly for the forage grass Urochloa ruziziensis based on single-molecule real-time sequencing. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 50. | | PESSOA FILHO, M. A. C. de P.; SOUZA SOBRINHO, F. de; FRAGOSO, R. da R.; SILVA JUNIOR, O. B. da; FERREIRA, M. E. A draft genome assembly for the forage grass Urochloa ruziziensis based on single-molecule real-time sequencing. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.| Biblioteca(s): Embrapa Cerrados. |
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| 51. | | SILVA JUNIOR, O. B. da; PESSOA FILHO, M. A. C. de P.; SA, M. E. L.; DEAL, R.; RAGOUSSIS, I.; GRATTAPAGLIA, D.; RECH FILHO, E. L. Coupling in-depth genome annotations with genome editing technology for harnessing genomic variation to promote precision breeding in tropical soybean. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 52. | | SILVA JUNIOR, O. B. da; PESSOA FILHO, M. A. C. de P.; SA, M. E. L.; DEAL, R.; RAGOUSSIS, I.; GRATTAPAGLIA, D.; RECH FILHO, E. L. Coupling in-depth genome annotations with genome editing technology for harnessing genomic variation to promote precision breeding in tropical soybean. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.| Biblioteca(s): Embrapa Cerrados. |
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| 53. | | RESENDE, R. T.; RESENDE, M. D. V. de; SILVA, F. F. S.; AZEVEDO, C. F. A.; TAKAHASHI, E. K. T.; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D. Regional heritability mapping and genome-wide association identify loci for complex growth, wood and disease resistance traits in Eucalyptus. New Phytologist, v. 213, p. 1287-1300, 2017.| Biblioteca(s): Embrapa Florestas; Embrapa Recursos Genéticos e Biotecnologia. |
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| 54. | | SILVA JUNIOR, O. B.; SÁ, M. E. L.; PESSOA-FILHO, M.; MELO, C. L. P. de; ARIAS, C. A. A.; GRATTAPAGLIA, D.; RECH, E. L. Reference grade genome sequence assembly for a Brazilian soybean germplasm. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 10., 2019, Águas de Lindóia. Tema: Pesquisa e Inovação para o Desenvolvimento da Sociedade: resumos dos trabalhos. [S.l.]: Sociedade Brasileira de Melhoramento de Plantas, 2019. p. 64| Biblioteca(s): Embrapa Soja. |
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| 55. | | SILVA JUNIOR, O. B.; SÁ, M. E. L.; PESSOA-FILHO, M.; MELO, C. L. P. de; ARIAS, C. A. A.; GRATTAPAGLIA, D.; RECH, E. L. Reference grade genome sequence assembly for a Brazilian soybean germplasm. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 10., 2019, Águas de Lindóia. Tema: Pesquisa e Inovação para o Desenvolvimento da Sociedade: resumos dos trabalhos. [S.l.]: Sociedade Brasileira de Melhoramento de Plantas, 2019. p. 64| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 56. | | GRATTAPAGLIA, D.; SILVA JUNIOR, O. B. da; RESENDE, R. T.; CAPPA, E. P.; MÜLLER, B. S. F.; TAN, B.; ISIK, F.; RATCLIFFE, B.; EL-KASSABY, Y. A. Quantitative genetics and genomics converge to accelerate forest tree breeding. Frontiers in Plant Science, v. 9, article 1693, 2018. Na publicação: Orzenil B. Silva-Junior.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 57. | | BERGMANN, J. C.; BOARI, A. de J.; KRUGER, R. H.; TAVARES P.; TOGAWA, R. C.; SILVA JUNIOR, O. B. da; PAPPAS JUNIOR, G. J.; QUIRINO, B. F. New strategies to look at old problems: fatal yellowing in palm oil tree. In: BRAZILIAN BIOENERGY SCIENCE AND TECHNOLOGY CONFERENCE - BBEST, 1., 2011, Campos do Jordão, SP. [Anais...]. São Paulo: FAPESP, 2011. Não paginado.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 58. | | BERGMANN, J. C.; BOARI, A. de J.; KRUGER, R. H.; TAVARES, P.; TOGAWA, R. C.; SILVA JUNIOR, O. B. da; PAPPAS JUNIOR, G. J.; QUIRINO, B. F. New strategies to look at old problems: fatal yellowing in palm oil tree. In: BRAZILIAN BIOENERGY SCIENCE AND TECHNOLOGY CONFERENCE - BBEST, 1., 2011, Campos do Jordão, SP. [Anais...]. São Paulo: FAPESP, 2011. Não paginado.| Biblioteca(s): Embrapa Agroenergia; Embrapa Amazônia Oriental. |
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| 59. | | PESSOA FILHO, M. A. C. de P.; SOUZA SOBRINHO, F. de; FRAGOSO, R. da R.; SILVA JUNIOR, O. B. da; FERREIRA, M. E. A phased diploid genome assembly for the forage grass urochloa ruziziensis based on single-molecule real-time sequencing. In: PLANT AND ANIMAL GENOME CONFERENCE, 27., 2019, San Diego. Proceedings... Livingston, NJ: Scherago, 2019 Plant and Animal Genome XXVII Conference (PAG).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 60. | | PESSOA FILHO, M. A. C. de P.; SOUZA SOBRINHO, F. de; FRAGOSO, R. da R.; SILVA JUNIOR, O. B. da; FERREIRA, M. E. A phased diploid genome assembly for the forage grass urochloa ruziziensis based on single-molecule real-time sequencing. In: PLANT AND ANIMAL GENOME CONFERENCE, 27., 2019, San Diego. Proceedings... Livingston, NJ: Scherago, 2019 Plant and Animal Genome XXVII Conference (PAG).| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 110 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
26/03/2019 |
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Data da última atualização: |
21/06/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PESSOA FILHO, M. A. C. de P.; SOUZA SOBRINHO, F. de; FRAGOSO, R. da R.; SILVA JUNIOR, O. B. da; FERREIRA, M. E. |
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Afiliação: |
MARCO AURELIO CALDAS DE PINHO PESSO, CPAC; FAUSTO DE SOUZA SOBRINHO, CNPGL; RODRIGO DA ROCHA FRAGOSO, CPAC; ORZENIL BONFIM DA SILVA JUNIOR, Cenargen; MARCIO ELIAS FERREIRA, Cenargen. |
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Título: |
A phased diploid genome assembly for the forage grass urochloa ruziziensis based on single-molecule real-time sequencing. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: PLANT AND ANIMAL GENOME CONFERENCE, 27., 2019, San Diego. Proceedings... Livingston, NJ: Scherago, 2019 |
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Idioma: |
Inglês |
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Notas: |
Plant and Animal Genome XXVII Conference (PAG). |
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Conteúdo: |
Ruzigrass (Urochloa ruziziensis) is a diploid, tropical forage grass native to Africa, widely planted in Brazil, known for its high nutritional quality. It is closely related to important forage species of Urochloa, and plays a crucial role in the breeding program of brachiaria grasses, mostly focused on inter-specific hybirds. Previous studies from our group based on shallow Illumina sequencing resulted in the development of the first molecular markers for the species (Silva et al., 2013), as well as in assessments of germplasm diversity and structure (Pessoa-Filho et al., 2015). Assembly and analysis of complete plastid genomes for four Urochloa species allowed the characterization of their phylogenetic divergence (Pessoa-Filho et al., 2017). Here, we present a near-complete phased diploid genome assembly of the heterozygous ruzigrass clone C69. We used PacBio Sequel to generate over 13.3 million long reads (mean size 6.5 kbp), adding up to 87.5 Gbp of raw data (~142x coverage). The current diploid assembly using FALCON-Unzip contains ~603 Mbp in 3,539 primary contigs, with NG50 of 286 kbp, and covers 98.2% of the estimated haploid genome size of 615 Mbp for ruzigrass. In addition, 82% of the assembly could be phased as separate haplotypes, with ~500 Mbp resolved as haplotigs. Assessment of assembly completeness showed 95.2% BUSCO matches as complete, and 83.3% as complete single-copy. Ongoing research includes transcriptome assembly to aid gene prediction and annotation, anchoring of contigs in linkage maps, and Hi-C scaffolding. A high-quality, chromosome-scale genome assembly for ruzigrass will aid research groups in the development and application of genomic tools in breeding and genetics of brachiaria grasses. MenosRuzigrass (Urochloa ruziziensis) is a diploid, tropical forage grass native to Africa, widely planted in Brazil, known for its high nutritional quality. It is closely related to important forage species of Urochloa, and plays a crucial role in the breeding program of brachiaria grasses, mostly focused on inter-specific hybirds. Previous studies from our group based on shallow Illumina sequencing resulted in the development of the first molecular markers for the species (Silva et al., 2013), as well as in assessments of germplasm diversity and structure (Pessoa-Filho et al., 2015). Assembly and analysis of complete plastid genomes for four Urochloa species allowed the characterization of their phylogenetic divergence (Pessoa-Filho et al., 2017). Here, we present a near-complete phased diploid genome assembly of the heterozygous ruzigrass clone C69. We used PacBio Sequel to generate over 13.3 million long reads (mean size 6.5 kbp), adding up to 87.5 Gbp of raw data (~142x coverage). The current diploid assembly using FALCON-Unzip contains ~603 Mbp in 3,539 primary contigs, with NG50 of 286 kbp, and covers 98.2% of the estimated haploid genome size of 615 Mbp for ruzigrass. In addition, 82% of the assembly could be phased as separate haplotypes, with ~500 Mbp resolved as haplotigs. Assessment of assembly completeness showed 95.2% BUSCO matches as complete, and 83.3% as complete single-copy. Ongoing research includes transcriptome assembly to aid gene prediction and annotation, ... Mostrar Tudo |
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Palavras-Chave: |
Brasil. |
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Thesagro: |
Capim Urochloa; Genoma. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/194810/1/abstract-PAG-XXVII.pdf
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Marc: |
LEADER 02499nam a2200205 a 4500
001 2107476
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008 2019 bl uuuu u01u1 u #d
100 1 $aPESSOA FILHO, M. A. C. de P.
245 $aA phased diploid genome assembly for the forage grass urochloa ruziziensis based on single-molecule real-time sequencing.$h[electronic resource]
260 $aIn: PLANT AND ANIMAL GENOME CONFERENCE, 27., 2019, San Diego. Proceedings... Livingston, NJ: Scherago$c2019
500 $aPlant and Animal Genome XXVII Conference (PAG).
520 $aRuzigrass (Urochloa ruziziensis) is a diploid, tropical forage grass native to Africa, widely planted in Brazil, known for its high nutritional quality. It is closely related to important forage species of Urochloa, and plays a crucial role in the breeding program of brachiaria grasses, mostly focused on inter-specific hybirds. Previous studies from our group based on shallow Illumina sequencing resulted in the development of the first molecular markers for the species (Silva et al., 2013), as well as in assessments of germplasm diversity and structure (Pessoa-Filho et al., 2015). Assembly and analysis of complete plastid genomes for four Urochloa species allowed the characterization of their phylogenetic divergence (Pessoa-Filho et al., 2017). Here, we present a near-complete phased diploid genome assembly of the heterozygous ruzigrass clone C69. We used PacBio Sequel to generate over 13.3 million long reads (mean size 6.5 kbp), adding up to 87.5 Gbp of raw data (~142x coverage). The current diploid assembly using FALCON-Unzip contains ~603 Mbp in 3,539 primary contigs, with NG50 of 286 kbp, and covers 98.2% of the estimated haploid genome size of 615 Mbp for ruzigrass. In addition, 82% of the assembly could be phased as separate haplotypes, with ~500 Mbp resolved as haplotigs. Assessment of assembly completeness showed 95.2% BUSCO matches as complete, and 83.3% as complete single-copy. Ongoing research includes transcriptome assembly to aid gene prediction and annotation, anchoring of contigs in linkage maps, and Hi-C scaffolding. A high-quality, chromosome-scale genome assembly for ruzigrass will aid research groups in the development and application of genomic tools in breeding and genetics of brachiaria grasses.
650 $aCapim Urochloa
650 $aGenoma
653 $aBrasil
700 1 $aSOUZA SOBRINHO, F. de
700 1 $aFRAGOSO, R. da R.
700 1 $aSILVA JUNIOR, O. B. da
700 1 $aFERREIRA, M. E.
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