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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
20/01/2015 |
Data da última atualização: |
22/01/2020 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
SOUZA, M. M.; ZERLOTINI, A.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; SOMAVILLA, A. L.; MOKRY, F. B.; CESAR, A. S. M.; DINIZ, W. J. S.; MUDADU, M. A.; NICIURA, S. C. M.; COUTINHO, L. L.; REGITANO, L. C. A. |
Afiliação: |
UFSCar; ADHEMAR ZERLOTINI NETO, CNPTIA; UFSCar; UFSCar; Unesp Jaboticabal; UFSCar; Universidade de São Paulo, Piracicaba; UFSCar; MAURICIO DE ALVARENGA MUDADU, CPPSE; SIMONE CRISTINA MEO NICIURA, CPPSE; Universidade de São Paulo, Piracicaba; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
Título: |
Monoallelic expression of NNAT gene in Nelore steers skeletal muscle. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: WORLD CONGRESS OF GENETICS APPLIED TO LIVESTOCK PRODUCTION, 10.; 2014, Vancouver. Proceedings... Vancouver: American Society of Animal Science, 2014. |
Páginas: |
Não paginado. |
Idioma: |
Inglês |
Notas: |
WCGALP 2014. |
Conteúdo: |
ABSTRACT: The NNAT gene plays a role on adipogenesis, brain development and insulin secretion. NNAT is described as paternal imprinted in bovine fetal tissue, but it is still underexplored in adult tissues. In order to improve the understanding of NNAT expression, muscle from Nellore was used to assess its allelic expression. Genomic DNA from 38 steers was genotyped for identification of heterozygous individuals with known allele origin. Total mRNA from muscle was extracted and sequenced by HiScanSQ, and afterwards the reads of each allele were counted for each animal. A total of 8 reciprocal heterozygous had more than 10 reads for the chosen SNP. All animals showed monoallelic expression, being 5 with paternal allele expressed, while 7 had the G allele expressed. These results demonstrate that NNAT may not have the paternal imprint conserved in adult bovine muscle, and it suggests a G allele-dependent expression. |
Palavras-Chave: |
Neuronatin. |
Thesaurus Nal: |
Genomic imprinting; Zebu. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/116007/1/monoallelic-Souza.pdf
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Marc: |
LEADER 01869nam a2200301 a 4500 001 2006155 005 2020-01-22 008 2014 bl uuuu u00u1 u #d 100 1 $aSOUZA, M. M. 245 $aMonoallelic expression of NNAT gene in Nelore steers skeletal muscle.$h[electronic resource] 260 $aIn: WORLD CONGRESS OF GENETICS APPLIED TO LIVESTOCK PRODUCTION, 10.; 2014, Vancouver. Proceedings... Vancouver: American Society of Animal Science$c2014 300 $aNão paginado. 500 $aWCGALP 2014. 520 $aABSTRACT: The NNAT gene plays a role on adipogenesis, brain development and insulin secretion. NNAT is described as paternal imprinted in bovine fetal tissue, but it is still underexplored in adult tissues. In order to improve the understanding of NNAT expression, muscle from Nellore was used to assess its allelic expression. Genomic DNA from 38 steers was genotyped for identification of heterozygous individuals with known allele origin. Total mRNA from muscle was extracted and sequenced by HiScanSQ, and afterwards the reads of each allele were counted for each animal. A total of 8 reciprocal heterozygous had more than 10 reads for the chosen SNP. All animals showed monoallelic expression, being 5 with paternal allele expressed, while 7 had the G allele expressed. These results demonstrate that NNAT may not have the paternal imprint conserved in adult bovine muscle, and it suggests a G allele-dependent expression. 650 $aGenomic imprinting 650 $aZebu 653 $aNeuronatin 700 1 $aZERLOTINI, A. 700 1 $aTIZIOTO, P. C. 700 1 $aOLIVEIRA, P. S. N. 700 1 $aSOMAVILLA, A. L. 700 1 $aMOKRY, F. B. 700 1 $aCESAR, A. S. M. 700 1 $aDINIZ, W. J. S. 700 1 $aMUDADU, M. A. 700 1 $aNICIURA, S. C. M. 700 1 $aCOUTINHO, L. L. 700 1 $aREGITANO, L. C. A.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
20/01/2006 |
Data da última atualização: |
20/01/2006 |
Autoria: |
MARTIN NETO, L.; SIMÕES, M. L.; FERREIRA, J. A.; MILORI, D. M. B. P.; SILVA, W. T. L. da; PEREZ, M. G.; NASCIMENTO, O. R.; BAYER, C.; MIELNICZUK, J. |
Título: |
Applications of spectroscopies in humic substances research. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
In: HUMIC SCIENCE AND TECHNOLOGY, 8., 2005, Boston. Aggregation and physiological effects: [abstracts]. Boston: Northeastern University, 2005. |
Idioma: |
Inglês |
Conteúdo: |
Presents two recents contributions: the use of spin label molecules as probes to identify hydrophobic interactions of humic substances and spectroscopic analysis of humic substances and organo interactions of humic substances. |
Palavras-Chave: |
EMBRAPA; EPR; NMR. |
Thesaurus NAL: |
humic substances. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01006naa a2200265 a 4500 001 1029282 005 2006-01-20 008 2005 bl --- 0-- u #d 100 1 $aMARTIN NETO, L. 245 $aApplications of spectroscopies in humic substances research. 260 $c2005 520 $aPresents two recents contributions: the use of spin label molecules as probes to identify hydrophobic interactions of humic substances and spectroscopic analysis of humic substances and organo interactions of humic substances. 650 $ahumic substances 653 $aEMBRAPA 653 $aEPR 653 $aNMR 700 1 $aSIMÕES, M. L. 700 1 $aFERREIRA, J. A. 700 1 $aMILORI, D. M. B. P. 700 1 $aSILVA, W. T. L. da 700 1 $aPEREZ, M. G. 700 1 $aNASCIMENTO, O. R. 700 1 $aBAYER, C. 700 1 $aMIELNICZUK, J. 773 $tIn: HUMIC SCIENCE AND TECHNOLOGY, 8., 2005, Boston. Aggregation and physiological effects: [abstracts]. Boston: Northeastern University, 2005.
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