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Registro Completo |
Biblioteca(s): |
Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
Data corrente: |
11/09/2023 |
Data da última atualização: |
11/09/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
HAACH, V.; BASTOS, A. P. A.; GAVA, D.; FONSECA, F. M. da; MORES, M. A. Z.; COLDEBELLA, A.; FRANCO, A. C.; SCHAEFER, R. |
Afiliação: |
VANESSA HAACH, Universidade Federal do Rio Grande do Sul; ANA PAULA ALMEIDA BASTOS, CNPSA; DANIELLE GAVA, CNPSA; FRANCISCO NOE DA FONSECA, GEM; MARCOS ANTONIO ZANELLA MORES, CNPSA; ARLEI COLDEBELLA, CNPSA; ANA CLÁUDIA FRANCO, Universidade Federal do Rio Grande do Sul; REJANE SCHAEFER, CNPSA. |
Título: |
A polyvalent virosomal infuenza vaccine induces broad cellular and humoral immunity in pigs. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Virology Journal, v. 20, article number 181, 2023. |
DOI: |
https://doi.org/10.1186/s12985-023-02153-5 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Background: Influenza A virus (IAV) is endemic in pigs globally and co-circulation of genetically and antigenically diverse virus lineages of subtypes H1N1, H1N2 and H3N2 is a challenge for the development of effective vaccines. Virosomes are virus-like particles that mimic virus infection and have proven to be a successful vaccine platform against several animal and human viruses. Methods: This study evaluated the immunogenicity of a virosome-based influenza vaccine containing the surface glycoproteins of H1N1 pandemic, H1N2 and H3N2 in pigs. Results: A robust humoral and cellular immune response was induced against the three IAV subtypes in pigs after two vaccine doses. The influenza virosome vaccine elicited hemagglutinin-specific antibodies and virus-neutralizing activity. Furthermore, it induced a significant maturation of macrophages, and proliferation of B lymphocytes, effector and central memory CD4+ and CD8+ T cells, and CD8+ T lymphocytes producing interferon-γ. Also, the vaccine demonstrated potential to confer long-lasting immunity until the market age of pigs and proved to be safe and non-cytotoxic to pigs. Conclusions: This virosome platform allows flexibility to adjust the vaccine content to reflect the diversity of circulating IAVs in swine in Brazil. The vaccination of pigs may reduce the impact of the disease on swine production and the risk of swine-to-human transmission. |
Palavras-Chave: |
Cellular immunity; Virosomal vaccine. |
Thesagro: |
Imunidade; Suíno; Vacina. |
Thesaurus Nal: |
Humoral immunity; Influenza A virus; Swine. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156557/1/final10093.pdf
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Marc: |
LEADER 02316naa a2200313 a 4500 001 2156557 005 2023-09-11 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1186/s12985-023-02153-5$2DOI 100 1 $aHAACH, V. 245 $aA polyvalent virosomal infuenza vaccine induces broad cellular and humoral immunity in pigs.$h[electronic resource] 260 $c2023 520 $aAbstract: Background: Influenza A virus (IAV) is endemic in pigs globally and co-circulation of genetically and antigenically diverse virus lineages of subtypes H1N1, H1N2 and H3N2 is a challenge for the development of effective vaccines. Virosomes are virus-like particles that mimic virus infection and have proven to be a successful vaccine platform against several animal and human viruses. Methods: This study evaluated the immunogenicity of a virosome-based influenza vaccine containing the surface glycoproteins of H1N1 pandemic, H1N2 and H3N2 in pigs. Results: A robust humoral and cellular immune response was induced against the three IAV subtypes in pigs after two vaccine doses. The influenza virosome vaccine elicited hemagglutinin-specific antibodies and virus-neutralizing activity. Furthermore, it induced a significant maturation of macrophages, and proliferation of B lymphocytes, effector and central memory CD4+ and CD8+ T cells, and CD8+ T lymphocytes producing interferon-γ. Also, the vaccine demonstrated potential to confer long-lasting immunity until the market age of pigs and proved to be safe and non-cytotoxic to pigs. Conclusions: This virosome platform allows flexibility to adjust the vaccine content to reflect the diversity of circulating IAVs in swine in Brazil. The vaccination of pigs may reduce the impact of the disease on swine production and the risk of swine-to-human transmission. 650 $aHumoral immunity 650 $aInfluenza A virus 650 $aSwine 650 $aImunidade 650 $aSuíno 650 $aVacina 653 $aCellular immunity 653 $aVirosomal vaccine 700 1 $aBASTOS, A. P. A. 700 1 $aGAVA, D. 700 1 $aFONSECA, F. M. da 700 1 $aMORES, M. A. Z. 700 1 $aCOLDEBELLA, A. 700 1 $aFRANCO, A. C. 700 1 $aSCHAEFER, R. 773 $tVirology Journal$gv. 20, article number 181, 2023.
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Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
05/04/2021 |
Data da última atualização: |
10/06/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
CÔNSOLO, N. R. B.; BUARQUE, V. L. M.; SILVA, J.; POLETI, M. D.; BARBOSA, L. C. G. S.; HIGUERA-PADILLA, A.; GOMEZ, J. F. M.; COLNAGO, L. A.; GERRARD, D. E.; SARAN NETO, A.; SILVA, S. L. |
Afiliação: |
LUIZ ALBERTO COLNAGO, CNPDIA. |
Título: |
Muscle and liver metabolomic signatures associated with residual feed intake in Nellore cattle. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Animal Feed Science and Technology, v. 271, 114757, 2021. |
ISSN: |
0377-8401 |
DOI: |
https://doi.org/10.1016/j.anifeedsci.2020.114757 |
Idioma: |
Inglês |
Conteúdo: |
Identification of those cattle genetics with superior feed efficiency is necessary to increase the productivity of the entire beef industry. The aim of this study was to investigate metabolites profiling in the liver and muscle to identify physiological changes in animals in the livestock system with low or high residual feed intake. Liver and muscle were collected after slaughter from Nellore males, with high and low residual feed intake (H-RFI, n = 10 and L-RFI, n = 10, respectively). Metabolites were extracted with cold methanol/water solution (4:3 v/v). Metabolomics analyses were performed by 1D 1 H nuclear magnetic resonance. Metabolites were identified using Chenomx and analysed using MetaboAnalyst 4.0. The residual feed intake classification did not change most of animal performance traits; however, animals with lower residual feed intake presented greater carcass dressing, lower carcass fat deposition. Sixty-three compounds were identified in the liver and 31 in muscle. Coexpression network analysis was carried out and identified four metabolite network modules correlated with the phenotypic traits, indicating common metabolites with a variate important projection score that influences residual feed intake. Metabolites in liver samples was correlated with energy and protein metabolism and on muscle with fat metabolism. Differences on energetic metabolism, including of carbohydrate- and fat-correlated compounds, according to residual feed intake can be assessed by importance of concentration of citrate, isocitrate, glucose-6-phosphate, nicotinamide adenine dinucleotide + hydrogen and creatine phosphate (in liver) and choline, glycine, glycerol, malonate, glucose-6- phosphate and 3-hydroxybutyrate (in muscle). Moreover, differences on protein metabolism, MenosIdentification of those cattle genetics with superior feed efficiency is necessary to increase the productivity of the entire beef industry. The aim of this study was to investigate metabolites profiling in the liver and muscle to identify physiological changes in animals in the livestock system with low or high residual feed intake. Liver and muscle were collected after slaughter from Nellore males, with high and low residual feed intake (H-RFI, n = 10 and L-RFI, n = 10, respectively). Metabolites were extracted with cold methanol/water solution (4:3 v/v). Metabolomics analyses were performed by 1D 1 H nuclear magnetic resonance. Metabolites were identified using Chenomx and analysed using MetaboAnalyst 4.0. The residual feed intake classification did not change most of animal performance traits; however, animals with lower residual feed intake presented greater carcass dressing, lower carcass fat deposition. Sixty-three compounds were identified in the liver and 31 in muscle. Coexpression network analysis was carried out and identified four metabolite network modules correlated with the phenotypic traits, indicating common metabolites with a variate important projection score that influences residual feed intake. Metabolites in liver samples was correlated with energy and protein metabolism and on muscle with fat metabolism. Differences on energetic metabolism, including of carbohydrate- and fat-correlated compounds, according to residual feed intake can be assessed by imp... Mostrar Tudo |
Palavras-Chave: |
Muscle; Residual feed intake. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02667naa a2200289 a 4500 001 2131049 005 2022-06-10 008 2021 bl uuuu u00u1 u #d 022 $a0377-8401 024 7 $ahttps://doi.org/10.1016/j.anifeedsci.2020.114757$2DOI 100 1 $aCÔNSOLO, N. R. B. 245 $aMuscle and liver metabolomic signatures associated with residual feed intake in Nellore cattle.$h[electronic resource] 260 $c2021 520 $aIdentification of those cattle genetics with superior feed efficiency is necessary to increase the productivity of the entire beef industry. The aim of this study was to investigate metabolites profiling in the liver and muscle to identify physiological changes in animals in the livestock system with low or high residual feed intake. Liver and muscle were collected after slaughter from Nellore males, with high and low residual feed intake (H-RFI, n = 10 and L-RFI, n = 10, respectively). Metabolites were extracted with cold methanol/water solution (4:3 v/v). Metabolomics analyses were performed by 1D 1 H nuclear magnetic resonance. Metabolites were identified using Chenomx and analysed using MetaboAnalyst 4.0. The residual feed intake classification did not change most of animal performance traits; however, animals with lower residual feed intake presented greater carcass dressing, lower carcass fat deposition. Sixty-three compounds were identified in the liver and 31 in muscle. Coexpression network analysis was carried out and identified four metabolite network modules correlated with the phenotypic traits, indicating common metabolites with a variate important projection score that influences residual feed intake. Metabolites in liver samples was correlated with energy and protein metabolism and on muscle with fat metabolism. Differences on energetic metabolism, including of carbohydrate- and fat-correlated compounds, according to residual feed intake can be assessed by importance of concentration of citrate, isocitrate, glucose-6-phosphate, nicotinamide adenine dinucleotide + hydrogen and creatine phosphate (in liver) and choline, glycine, glycerol, malonate, glucose-6- phosphate and 3-hydroxybutyrate (in muscle). Moreover, differences on protein metabolism, 653 $aMuscle 653 $aResidual feed intake 700 1 $aBUARQUE, V. L. M. 700 1 $aSILVA, J. 700 1 $aPOLETI, M. D. 700 1 $aBARBOSA, L. C. G. S. 700 1 $aHIGUERA-PADILLA, A. 700 1 $aGOMEZ, J. F. M. 700 1 $aCOLNAGO, L. A. 700 1 $aGERRARD, D. E. 700 1 $aSARAN NETO, A. 700 1 $aSILVA, S. L. 773 $tAnimal Feed Science and Technology$gv. 271, 114757, 2021.
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