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| 8. |  | SILVA, R. R. V. Composição, distribuição por ambiente e dados biológicos da avifauna registrada no Complexo Esportivo do SESI, em Caxias do Sul, Rio Grande do Sul, Brasil. Perspectiva, Erechim, v. 29, n. 105, p. 43-51, mar. 2005.| Biblioteca(s): Embrapa Florestas. |
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| 15. | | GOMES, L. A. A.; SILVA, R. R.; MASSAROTO, J. A. Produção de mudas de pepino e tomate utilizando diferentes doses de aduboa foliar bioplus. Horticultura Brasileira, Brasília, v. 22, n. 2, jul. 2004. Suplemento 2. Trabalho apresentado no 44º Congresso Brasileiro de Olericultura, 2004. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 22, n. 2, p. 486, jul. 2004. Suplemento.| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 286 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
12/05/2021 |
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Data da última atualização: |
13/05/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ANDRADE, J. de S.; MOREIRA, E. M.; SILVA, G. M. da; SCHNEIDER, A.; NUNES, V. R. R.; SILVA, R. R. da; PFEIFER, L. F. M. |
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Afiliação: |
JÉSSICA DE SOUZA ANDRADE, Bionorte, Programa de Pós-Graduação em Biodiversidade e Biotecnologia, Porto Velho, RO; ELIZÂNGELA MÍRIAN MOREIRA, Universidade Federal do Amazonas (UFAM); GEORGE MOREIRA DA SILVA, Universidade Federal de Rondônia (UNIR); AUGUSTO SCHNEIDER, Universidade Federal de Pelotas (UFPel); VANESSA RACHELE RIBEIRO NUNES, CPAF-RO; RENATA REIS DA SILVA, CPAF-RO; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO. |
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Título: |
Uterine health and fertility of timed AI postpartum Nelore beef cows raised in the Amazon biome. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Livestock Science, v. 249, 104528, July 2021. |
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ISSN: |
1871-1413 |
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DOI: |
https://doi.org/10.1016/j.livsci.2021.104528 |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated the effect of uterine health on the fertility of postpartum beef cows. Multiparous lactating Nelore cows (Bos indicus; n = 155) were subjected to a timed artificial insemination (TAI) protocol. Cows were included in a progesterone-estradiol based TAI protocol. On Day 0, before the beginning of the protocol, cows were subjected to evaluation of purulent vaginal discharge (PVD) and collection of uterine tissue using the cytobrush technique. Slides for polymorphonuclear (PMN) cells counting were prepared before the same cytobrush was used for RNA extraction and analysis of IL-1B, IL-6, IL-8, TNFa, GAPDH, and Bactin gene transcription. Cows were separated into 4 categories according to the proportion of PMN and PVD: 1) Healthy (HE; n = 87), cows with <- 4.75% PMN and PVD of 0; 2) Positive Purulent Vaginal Discharge (PVD+; n = 24), cows with < - 4.75% PMN and PVD >- 1; 3) Subclinical Endometritis (SCE; n = 29), cows with > 4.75% PMN and PVD of 0, and; 4) Subclinical Endometritis + PVD+ (SCEP; n = 15), cows with > 4.75% PMN and PVD ? 1. Cows in the HE group had a greater (P < 0.05) pregnancy per AI (P/IA) than cows in SCE and SCEP groups. However, P/AI in cows from the PVD+ group was not different in comparison to HE and SCE groups (P > 0.05). Cows from the SCE and SCEP groups had a higher (P < 0.001) proportion of PMN cells in the uterus than HE and PVD+ groups. Relative transcription of IL-6 and TNFa did not change among groups (P > 0.05). In contrast, transcription of IL-8 was greater (P = 0.02) in SCEP than HE and SCE groups. The relative transcription IL-1B was greater (P < 0.05) in SCEP than in PVD+, but the cows in the HE and SCE groups were not different from the other groups (P = 0.08). Our results demonstrate that postpartum beef cows, raised in the Amazon biome, with a higher proportion of PMN cells have lower fertility. Moreover, ~ 40% of the postpartum cows included in TAI programs early postpartum had suboptimal uterine conditions (subclinical endometritis, purulent vaginal discharge, or both). MenosThis study evaluated the effect of uterine health on the fertility of postpartum beef cows. Multiparous lactating Nelore cows (Bos indicus; n = 155) were subjected to a timed artificial insemination (TAI) protocol. Cows were included in a progesterone-estradiol based TAI protocol. On Day 0, before the beginning of the protocol, cows were subjected to evaluation of purulent vaginal discharge (PVD) and collection of uterine tissue using the cytobrush technique. Slides for polymorphonuclear (PMN) cells counting were prepared before the same cytobrush was used for RNA extraction and analysis of IL-1B, IL-6, IL-8, TNFa, GAPDH, and Bactin gene transcription. Cows were separated into 4 categories according to the proportion of PMN and PVD: 1) Healthy (HE; n = 87), cows with <- 4.75% PMN and PVD of 0; 2) Positive Purulent Vaginal Discharge (PVD+; n = 24), cows with < - 4.75% PMN and PVD >- 1; 3) Subclinical Endometritis (SCE; n = 29), cows with > 4.75% PMN and PVD of 0, and; 4) Subclinical Endometritis + PVD+ (SCEP; n = 15), cows with > 4.75% PMN and PVD ? 1. Cows in the HE group had a greater (P < 0.05) pregnancy per AI (P/IA) than cows in SCE and SCEP groups. However, P/AI in cows from the PVD+ group was not different in comparison to HE and SCE groups (P > 0.05). Cows from the SCE and SCEP groups had a higher (P < 0.001) proportion of PMN cells in the uterus than HE and PVD+ groups. Relative transcription of IL-6 and TNFa did not change among groups (P > 0.05). In contrast, trans... Mostrar Tudo |
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Palavras-Chave: |
Citocina; Cytobrush; Inseminação artifical por tempo determinado; PMN cells; Timed artificial insemination (TAI); Uterine health. |
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Thesagro: |
Fertilidade Animal; Gado de Corte; Gado Nelore; Imunidade; Pós-Parto; Reprodução Animal; Sanidade Animal; Útero; Vaca de Corte. |
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Thesaurus NAL: |
Animal fertility; Animal reproduction; Beef cows; Cytokines; Immunity; Nellore; Postpartum period. |
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Categoria do assunto: |
G Melhoramento Genético |
|
Marc: |
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100 1 $aANDRADE, J. de S.
245 $aUterine health and fertility of timed AI postpartum Nelore beef cows raised in the Amazon biome.$h[electronic resource]
260 $c2021
520 $aThis study evaluated the effect of uterine health on the fertility of postpartum beef cows. Multiparous lactating Nelore cows (Bos indicus; n = 155) were subjected to a timed artificial insemination (TAI) protocol. Cows were included in a progesterone-estradiol based TAI protocol. On Day 0, before the beginning of the protocol, cows were subjected to evaluation of purulent vaginal discharge (PVD) and collection of uterine tissue using the cytobrush technique. Slides for polymorphonuclear (PMN) cells counting were prepared before the same cytobrush was used for RNA extraction and analysis of IL-1B, IL-6, IL-8, TNFa, GAPDH, and Bactin gene transcription. Cows were separated into 4 categories according to the proportion of PMN and PVD: 1) Healthy (HE; n = 87), cows with <- 4.75% PMN and PVD of 0; 2) Positive Purulent Vaginal Discharge (PVD+; n = 24), cows with < - 4.75% PMN and PVD >- 1; 3) Subclinical Endometritis (SCE; n = 29), cows with > 4.75% PMN and PVD of 0, and; 4) Subclinical Endometritis + PVD+ (SCEP; n = 15), cows with > 4.75% PMN and PVD ? 1. Cows in the HE group had a greater (P < 0.05) pregnancy per AI (P/IA) than cows in SCE and SCEP groups. However, P/AI in cows from the PVD+ group was not different in comparison to HE and SCE groups (P > 0.05). Cows from the SCE and SCEP groups had a higher (P < 0.001) proportion of PMN cells in the uterus than HE and PVD+ groups. Relative transcription of IL-6 and TNFa did not change among groups (P > 0.05). In contrast, transcription of IL-8 was greater (P = 0.02) in SCEP than HE and SCE groups. The relative transcription IL-1B was greater (P < 0.05) in SCEP than in PVD+, but the cows in the HE and SCE groups were not different from the other groups (P = 0.08). Our results demonstrate that postpartum beef cows, raised in the Amazon biome, with a higher proportion of PMN cells have lower fertility. Moreover, ~ 40% of the postpartum cows included in TAI programs early postpartum had suboptimal uterine conditions (subclinical endometritis, purulent vaginal discharge, or both).
650 $aAnimal fertility
650 $aAnimal reproduction
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650 $aPostpartum period
650 $aFertilidade Animal
650 $aGado de Corte
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650 $aImunidade
650 $aPós-Parto
650 $aReprodução Animal
650 $aSanidade Animal
650 $aÚtero
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653 $aCitocina
653 $aCytobrush
653 $aInseminação artifical por tempo determinado
653 $aPMN cells
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653 $aUterine health
700 1 $aMOREIRA, E. M.
700 1 $aSILVA, G. M. da
700 1 $aSCHNEIDER, A.
700 1 $aNUNES, V. R. R.
700 1 $aSILVA, R. R. da
700 1 $aPFEIFER, L. F. M.
773 $tLivestock Science$gv. 249, 104528, July 2021.
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