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21. | | HONER, M. R.; GOMES, A.; KOLLER, W. W.; SILVA, R. L. da. Southern oscilation teleconnections in the population dynamics of berne, larva of Dermatobia hominis, in Brazil. Revista Brasileira de Parasitologia Veterinaria, Sao Paulo, v.7, n.1, p.31-35, 1998. CNPGC. Biblioteca(s): Embrapa Gado de Corte. |
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22. | | GOMES, A.; KOLLER, W. W.; HONER, M. R.; SILVA, R. L. da. Flutuacao populacional da mosca Cochliomya hominivorax (Coquerel, 1859) (Diptera: calliphoridae) capturada em armadilhas orientadas pelo vento (W.O.T.), no municipio de Campo Grande, MS. Revista Brasileira de Parasitologia Veterinaria, Sao Paulo, v.7, n.1, p.41-45, 1998. CNPGC. Biblioteca(s): Embrapa Gado de Corte. |
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24. | | SILVA, R. L. da; XAVIER, A.; LEITE, H. G.; PIRES, I. E. Determinação do tamanho ótimo da parcela experimental pelos métodos da máxima curvatura modificado, do coeficiente de correlação intraclasse e da análise visula em testes clonais de eucalipto. Revista Árvore, Viçosa, v. 27, n. 5, p. 669-676, set./out. 2003. Biblioteca(s): Embrapa Florestas. |
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27. | | RESENDE, M. D. V. de; PIRES, I. E.; SILVA, R. L. da. Melhoramento do eucalipto. In: LOPES, M. A.; FÁVERO, A. P.; FERREIRA, M. A. J. da F.; FALEIRO, F. G.; FOLLE, S. M.; GUIMARÃES, E. P. (Ed.). Pré-melhoramento de plantas: estado da arte e experiências de sucesso. Brasília, DF: Embrapa Informação tecnológica; Embrapa Cerrados, 2011. p. 413-440. il. color. Biblioteca(s): Embrapa Florestas. |
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30. | | GOMES, A.; HONER, M. R.; KOLLER, W. W.; SILVA, R. L. da. Vetores de ovos de Dermatobia hominis (L.JR. 1781) (Diptera: cuterebridade) na regiao de cerrados do Mato Grosso do Sul, Brasil. Revista Brasileira de Parasitologia Veterinaria, Sao Paulo, v.7, n.1, p.37-40, 1998. CNPGC. Biblioteca(s): Embrapa Gado de Corte. |
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32. | | SOUZA, F. V. D.; SOUZA, E. H. de; KAYA, E.; VIEIRA, L. de J.; SILVA, R. L. da. Cryopreservation of pineapple Shoot Tips by the droplet vitrification technique. In: LOYOLA-VARGAS, V.r M; Ochoa-Alejo, N. (ed.). Plant Cell Culture Protocols, Methods in Molecular Biology. New York, NY: Humana Press, 2018. 269-277 v.1815. Cap. 18. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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35. | | SILVA, R. L. da; MATOS, A. DE O.; RIBEIRO, D. A.; XISTO, G. J.; LOURENCO, J. N. de P. Diversidade florística em quintais agroflorestais de quatro localidades, Parintins - Amazonas. In: CONGRESSO NACIONAL DE BOTÂNICA, 61., 2010, Manaus. Diversidade vegetal brasileira: conhecimento, conservação e uso. Manaus: SBB, 2010. 1 CD-ROM. Biblioteca(s): Embrapa Amazônia Ocidental. |
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37. | | OLIVEIRA, H.; SOUZA, T. F.; SILVA, M. A. da; SILVA, R. L. da; CANCADO, P. H. D. Avaliação de diferentes subprodutos de usinas sucroalcooleiras como substratos para o desenvolvimento de moscas-dos-estábulos: resultados preliminares. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 9., 2013, Campo Grande, MS. [Anais da..]. Campo Grande, MS: Embrapa Gado de Corte, 2013. (Embrapa Gado de Corte. Documentos, 204). Comissão organizadora: Denise Baptaglin Montagner, Grácia Maria Soares Rosinha, Rodrigo Carvalho Alva. Biblioteca(s): Embrapa Gado de Corte. |
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39. | | MATTOS, C. A. C. de; PINTO, W. da S.; CARDOSO, A. J. G.; SILVA, R. L. da; BRIENZA JUNIOR, S. Estratégias de planejamento a partir do diagnóstico rápido participativo e da análise SWOT: um estudo na comunidade de São Bento, Salinopólis - PA. Sociedade e Desenvolvimento Rural, v. 5, n. 2, p. 17-32, dez. 2011. Biblioteca(s): Embrapa Amazônia Oriental. |
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40. | | RODRIGUES, J. D.; SOUZA, T. F.; SILVA, M. A. da; SILVA, R. L. da; CANCADO, P. H. D. Estudo preliminar da flutuação populacional de mosca-dos-estábulos na Embrapa Gado de Corte, Campo Grande, MS. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 9., 2013, Campo Grande, MS. [Anais da..]. Campo Grande, MS: Embrapa Gado de Corte, 2013. (Embrapa Gado de Corte. Documentos, 204). Comissão organizadora: Denise Baptaglin Montagner, Grácia Maria Soares Rosinha, Rodrigo Carvalho Alva Biblioteca(s): Embrapa Gado de Corte. |
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Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
04/12/2017 |
Data da última atualização: |
04/12/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SILVA, R. L. da; SOUZA, E. H. de; VIEIRA, L. de J.; PELACANI, C. R.; SOUZA, F. V. D. |
Afiliação: |
RONILZE LEITE DA SILVA, EFS; EVERTON HILO DE SOUZA, CAPES; LÍVIA DE JESUS VIEIRA, CAPES; CLAUDINÉIA REGINA PELACANI, UEFS; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
Título: |
Cryopreservation of pollen of wild pineapple accessions. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Scientia Horticulturae, v.219, p.326-334, May 2017. |
Idioma: |
Inglês |
Conteúdo: |
Cryopreservation of pollen is an additional way to conserve many species. In particular, it can be used as an alternative strategy to preserve the genetic variation of the genus Ananas. The aim of this study was to cryopreserve pollen from wild pineapple accessions to conserve a gene pool of the genus Ananas, as well as to minimize problems of asynchronous flowering among accessions preserved in a germplasm bank. Accessions of all botanical varieties were used to investigate different protocols for freezing pollen, as well as to determine their viability in vitro/in vivo during the investigation and after freezing in liquid nitrogen. Anthers with pollen of the different accessions were dehydrated and then immersed in liquid nitrogen for periods of 24 h, 60 days and 120 days. After freezing, the viability percentage and pollen tube length were determined. In vivo viability tests were performed through 24 pollinations involving different accessions. The results showed that the cryopreservation of pollen was efficient, attaining in vitro germination rates ranging from 8.24% to 94.25%, with average of 62.67% achieved 120 days after cryopreservation and pollen tube lengths longer than 0.23 mm after this same period. In the in vivo pollinations with cryopreserved grains, the seed formation rate was 70.83% for the different combinations. It was possible to cryopreserve pollen from all the pineapple accessions investigated after dehydration for 6 h on silica gel, with significant in vitro germination percentages and production of viable seeds. MenosCryopreservation of pollen is an additional way to conserve many species. In particular, it can be used as an alternative strategy to preserve the genetic variation of the genus Ananas. The aim of this study was to cryopreserve pollen from wild pineapple accessions to conserve a gene pool of the genus Ananas, as well as to minimize problems of asynchronous flowering among accessions preserved in a germplasm bank. Accessions of all botanical varieties were used to investigate different protocols for freezing pollen, as well as to determine their viability in vitro/in vivo during the investigation and after freezing in liquid nitrogen. Anthers with pollen of the different accessions were dehydrated and then immersed in liquid nitrogen for periods of 24 h, 60 days and 120 days. After freezing, the viability percentage and pollen tube length were determined. In vivo viability tests were performed through 24 pollinations involving different accessions. The results showed that the cryopreservation of pollen was efficient, attaining in vitro germination rates ranging from 8.24% to 94.25%, with average of 62.67% achieved 120 days after cryopreservation and pollen tube lengths longer than 0.23 mm after this same period. In the in vivo pollinations with cryopreserved grains, the seed formation rate was 70.83% for the different combinations. It was possible to cryopreserve pollen from all the pineapple accessions investigated after dehydration for 6 h on silica gel, with significant in... Mostrar Tudo |
Thesagro: |
Abacaxi. |
Categoria do assunto: |
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LEADER 02070naa a2200181 a 4500 001 2081351 005 2017-12-04 008 2017 bl uuuu u00u1 u #d 100 1 $aSILVA, R. L. da 245 $aCryopreservation of pollen of wild pineapple accessions.$h[electronic resource] 260 $c2017 520 $aCryopreservation of pollen is an additional way to conserve many species. In particular, it can be used as an alternative strategy to preserve the genetic variation of the genus Ananas. The aim of this study was to cryopreserve pollen from wild pineapple accessions to conserve a gene pool of the genus Ananas, as well as to minimize problems of asynchronous flowering among accessions preserved in a germplasm bank. Accessions of all botanical varieties were used to investigate different protocols for freezing pollen, as well as to determine their viability in vitro/in vivo during the investigation and after freezing in liquid nitrogen. Anthers with pollen of the different accessions were dehydrated and then immersed in liquid nitrogen for periods of 24 h, 60 days and 120 days. After freezing, the viability percentage and pollen tube length were determined. In vivo viability tests were performed through 24 pollinations involving different accessions. The results showed that the cryopreservation of pollen was efficient, attaining in vitro germination rates ranging from 8.24% to 94.25%, with average of 62.67% achieved 120 days after cryopreservation and pollen tube lengths longer than 0.23 mm after this same period. In the in vivo pollinations with cryopreserved grains, the seed formation rate was 70.83% for the different combinations. It was possible to cryopreserve pollen from all the pineapple accessions investigated after dehydration for 6 h on silica gel, with significant in vitro germination percentages and production of viable seeds. 650 $aAbacaxi 700 1 $aSOUZA, E. H. de 700 1 $aVIEIRA, L. de J. 700 1 $aPELACANI, C. R. 700 1 $aSOUZA, F. V. D. 773 $tScientia Horticulturae$gv.219, p.326-334, May 2017.
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