Data/hora: 12/06/2024 03:38:27

Biblioteca(s): | Embrapa Meio Ambiente.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 2
Data corrente: | 18/12/2013
Data da última atualização: | 28/02/2014
Autoria: | MELO, I. S. de; SANTOS, S. N.; ROSA, L. H.; PARMA, M. M.; SILVA, L. J.; QUEIROZ, S. C. do N. de; PELLIZARI, V. H.
Afiliação: | ITAMAR SOARES DE MELO, CNPMA; SUIKINAI NOBRE SANTOS, ESALQ-USP; LUIZ HENRIQUE ROSA, ICB-UFMG; MARCIA MARIA PARMA, CNPMA; LEONARDO J SILVA; SONIA CLAUDIA DO NASCIMENTO DE QUEIROZ, CNPMA; VIVAN HELENA PELLIZARI, IO-USP.
Título: | Isolation and biological activities of an endophytic Mortierella alpina strain from the Antarctic moss Schistidium antarctici.
Ano de publicação: | 2014
Fonte/Imprenta: | Extremophiles, Tokyo, v. 18, n. 1, p. 15-23, 2014.
Idioma: | Inglês
Conteúdo: | The Antarctic endophytic fungus (strain ITA1-CCMA 952) was isolated from the moss Schistidium antarctici found in Admiralty Bay, King George Island, Antarctica. Strain ITA1-CCMA 952 was assigned to the specie Mortierella alpina by phylogenetic analysis based on 18S rRNA gene sequences. This strain produces high levels of polyunsaturated fatty acids (PUFAs), including y-(gamma) linolenic acid and arachidonic acid, which when combined represents 48.3 % of the total fatty acid content. Fungal extracts demonstrated strong antioxidant activity with the EC50 value of 48.7 lg mL-1 and also a strong antibacterial activity, mainly against the following bacteria: Escherichia coli, with a MIC of 26.9 lg mL-1 and Pseudomonas aeruginosa and Enterococcus faecalis, both with a MIC of 107 lg mL-1. A GC-MS analysis of the chloroform fraction obtained from the crude extract revealed the presence of potential antimicrobials (Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) and Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl)) as the major compounds. Therefore, the M. alpina strain ITA1-CCMA 952 is a promising fungus for the biotechnological production of antibiotics, antioxidant substances and PUFAs. This study highlights the need for more research in extreme environments, such as Antarctica.
Palavras-Chave: | Antibacterial activity; PUFAs; Pyrrolopyrazine alkaloids; Schistidium antarctici.
Thesagro: | Bactéria; Fungo.
Thesaurus Nal: | Antarctica; Antibacterial properties; Entomopathogenic fungi; Mortierella.
Categoria do assunto: | --
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (12857-1) - UPC - AP - DD -  -  -  - DIS