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| Registros recuperados : 356 | |
| 83. |  | SILVA, G. C.; SCHMITZ, R.; SILVA, L. C. da; CARPANINI, G. G.; MAGALHÃES, R. C. Desempenho de cultivares para produção de milho verde na agricultura familiar do Sul de Roraima. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 14, n. 2, p. 273-282, 2015.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 90. | | SILVA, L. C.; AHGHINONI, I.; LIMA, C. V. S. de; MANTOVANI, A.; CHAVARRIA, G. Disponibilidade de cobre à videira consorciada com gramíneas em solo com alta concentração do metal. Synergismus scyentifica UTFPR, Pato Branco, v. 4, n. 1, p. 1-4, 2009. 4 1-4 Online.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 92. | | SILVA, L. C. F. da; SANTOS, S. A.; SILVA, G. A. da; RAVAGLIA, A. G. Diagnóstico rápido de pastagens invadidas por aromita (acacia farnesiana) na sub-região de Miranda, MS. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PANTANAL, 5.; SEMANA DA BIOLOGIA, 10., 2011, Corumbá. Resumos ... Corumbá: Embrapa Pantanal, 2011. p. 16. (Embrapa Pantanal. Documentos, 113).| Biblioteca(s): Embrapa Pantanal. |
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| 93. | | SILVA, L. C.; COSTA, C.; SANTOS, S. A.; COMASTRI FILHO, J. A.; GARCIA, J. B. Dormência de sementes da gramínia nativa Mesosetum chaseae, sub-região da Nhecolândia, Pantanal. In: MOSTRA CIENTÍFICA DE CIENTÍFICAS AGRÁRIAS, 3.; MOSTRA CIENTÍFICA DA FMVZ, 11.; REUNIÃO CIENTÍFICA DA FAZENDA LAGEADO, 14. Botucatu, 2007. [Resumos]...[Botucatu]: [Unesp], 2007.| Biblioteca(s): Embrapa Pantanal. |
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| 96. | | MARINI, J.; SANTOS, H. P. dos; SOUZA, D. A.; ZART, M.; SILVA, L. C. da. Efeito de diferentes doses de extrato de alho na superação de dormência de Caberbet Sauvignon (Vitis vinifera L.). In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 8.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 4., 2010, Bento Gonçalves. Resumos. Bento Gonçalves: Embrapa Uva e Vinho, 2010. p. p. 29. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 97. | | TABET, A. F.; CORTOPASSI, S. R. G.; GREGORY, L.; SILVA, L. C. L. C. Correção do prolapso cervical recorrente por meio da laparoscopia em ovelha. Revista Brasileira de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 58, supl. 2, p. 106, set. 2006. Edição de Resumos do 7o. Congresso do Colégio Brasileiro de Cirurgia e Anestesiologia Veterinária, - CBCV, Santos, SP, set. 2006.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 356 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
06/05/2019 |
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Data da última atualização: |
06/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
COELHO, S. V. B.; ROSA, S. D. V. F. da; FANTAZZINI, T. B.; BAUTE, J. L.; SILVA, L. C. |
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Afiliação: |
Stefânia Vilas Boas Coelho, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; Tatiana Botelho Fantazzini, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; Júlia Lima Baute, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; Luciano Coutinho Silva, Departamento de Biologia Celular e Molecular/Universidade Federal da Paraíba - UFPB. |
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Título: |
Cryopreservation in Coffea canephora Pierre seeds: slow and fast cooling. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Ciência e Agrotecnologia, v. 42, n. 6, p. 588-597, Nov/Dec. 2018 |
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Idioma: |
Português |
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Notas: |
Título em português Criopreservação em sementes de Coffea canephora Pierre: Resfriamento lento e rápido. |
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Conteúdo: |
Coffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1 and -5 ºC min-1 until the end temperatures of -40 ºC, -50 ºC and -60 ºC, by means of a bio freezer and subsequently immersed in liquid nitrogen. In the second experiment, the best result was selected of the first experiment and compared with the rapid cooling, in which dried seeds, with 0.25 g g-1 of water content, were immersed directly into liquid nitrogen. Physiological and biochemical alterations occurring in the seeds after cryopreservation were evaluated. Coffea canephora seeds respond better to cryopreservation by rapid cooling, when compared to slow cooling. Drying, one of the cryopreservation steps does not affect the viability of Coffea canephora Pierre seeds, when these seeds are dried to 0.25 g g-1 of water content. Catalase and esterase enzymes are good biochemical markers for cryopreserved coffee seeds and their activity is greater in larger seed physiological quality. MenosCoffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1 and -5 ºC min-1 until the end temperatures of -40 ºC, -50 ºC and -60 ºC, by means of a bio freezer and subsequently immersed in liquid nitrogen. In the second experiment, the best result was selected of the first experiment and compared with the rapid cooling, in which dried seeds, with 0.25 g g-1 of water content, were immersed directly into liquid nitrogen. Physiological and biochemical alterations occurring in the seeds after cryopreservation were evaluated. Coffea canephora seeds respond better to cryopreservation by rapid cooling, when compared to slow cooling. Drying, one of the cryopreservation steps does not affect the viability of Coffea canephora Pierre seeds, when these seeds are dried to 0.25 g g-1 of water content. Cat... Mostrar Tudo |
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Palavras-Chave: |
Cristal de gelo; Drying in silica gel; Ice crystals; Isoenzymes; Physiological quality; Qualidade fisiológica; Secagem em sílica gel; Semente recalcitrante. |
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Thesagro: |
Isoenzima; Secagem. |
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Thesaurus NAL: |
Recalcitrant seeds. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/196979/1/Cryopreservation-in-Coffea-canephora-Pierre-seeds.pdf
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Marc: |
LEADER 02666naa a2200313 a 4500
001 2108759
005 2019-05-06
008 2018 bl uuuu u00u1 u #d
100 1 $aCOELHO, S. V. B.
245 $aCryopreservation in Coffea canephora Pierre seeds$bslow and fast cooling.$h[electronic resource]
260 $c2018
500 $aTítulo em português Criopreservação em sementes de Coffea canephora Pierre: Resfriamento lento e rápido.
520 $aCoffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1 and -5 ºC min-1 until the end temperatures of -40 ºC, -50 ºC and -60 ºC, by means of a bio freezer and subsequently immersed in liquid nitrogen. In the second experiment, the best result was selected of the first experiment and compared with the rapid cooling, in which dried seeds, with 0.25 g g-1 of water content, were immersed directly into liquid nitrogen. Physiological and biochemical alterations occurring in the seeds after cryopreservation were evaluated. Coffea canephora seeds respond better to cryopreservation by rapid cooling, when compared to slow cooling. Drying, one of the cryopreservation steps does not affect the viability of Coffea canephora Pierre seeds, when these seeds are dried to 0.25 g g-1 of water content. Catalase and esterase enzymes are good biochemical markers for cryopreserved coffee seeds and their activity is greater in larger seed physiological quality.
650 $aRecalcitrant seeds
650 $aIsoenzima
650 $aSecagem
653 $aCristal de gelo
653 $aDrying in silica gel
653 $aIce crystals
653 $aIsoenzymes
653 $aPhysiological quality
653 $aQualidade fisiológica
653 $aSecagem em sílica gel
653 $aSemente recalcitrante
700 1 $aROSA, S. D. V. F. da
700 1 $aFANTAZZINI, T. B.
700 1 $aBAUTE, J. L.
700 1 $aSILVA, L. C.
773 $tCiência e Agrotecnologia$gv. 42, n. 6, p. 588-597, Nov/Dec. 2018
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Embrapa Café (CNPCa) |
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