| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Milho e Sorgo. |
|
Data corrente: |
29/05/2014 |
|
Data da última atualização: |
24/05/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
VALICENTE, F. H.; SILVA, R. B. da. |
|
Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS; ROSANE BEZERRA DA SILVA, BOLSISTA. |
|
Título: |
fAFLP analysis of Brazilian Bacillus thuringiensis isolates. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
SpringerPlus, v. 3, n. 256, 2014. |
|
DOI: |
10.1186/2193-1801-3-256 |
|
Idioma: |
Inglês |
|
Conteúdo: |
A total of 65 Bacillus thuringiensis (Bt) isolates were subjected to analysis of genetic relationship using fAFLP (fluorescent Fragment Length Polymorphism), in order to determine the genetic diversity within a group of Bt strains. 26 strains from different subspecies were identified as it follows: 9 kindly provided by the USDA (United States Department of Agriculture), 9 kindly provided by the Institute Pasteur and eight from Embrapa Maize and Sorghum Bt Collection, and 39 strains with no subspecies information also from Embrapa’s Bt Collection. DNA sample was double digested with restriction enzymes EcoRI and MseI, and the fragments were linked to adapters. Selective amplification reactions were performed using five primer combinations and the amplified fragments were separated by gel electrophoresis on an ABI377 sequencer. Genetic distances were obtained by the complement of the Jaccard coefficient and the groups were performed by the UPGMA method. Five primer combinations generated 495 scorable fragments and 483 were found to be polymorphic. Out of 26 subspecies, strains 344 and T09 (B. thuringiensis subsp. tolworthi) showed the highest similarity (15%), while isolates HD3 B. thuringiensis subsp finitimus and T24 B. thuringiensis subsp neoleonensis were the most genetically distant (92%). B. thuringiensis isolates with no subspecies identification, found in samples from Goiás State showed higher similarity forming a group with an average distance of 6%, and the closest subspecies to this group was B. thuringiensis subsp thuringiensis (HD2) with 52% of similarity. This similarity may be due to the fact that these organism exchange genetic material by conjugation, and it is relatively common to have evolutionary characteristics of their ancestors. MenosA total of 65 Bacillus thuringiensis (Bt) isolates were subjected to analysis of genetic relationship using fAFLP (fluorescent Fragment Length Polymorphism), in order to determine the genetic diversity within a group of Bt strains. 26 strains from different subspecies were identified as it follows: 9 kindly provided by the USDA (United States Department of Agriculture), 9 kindly provided by the Institute Pasteur and eight from Embrapa Maize and Sorghum Bt Collection, and 39 strains with no subspecies information also from Embrapa’s Bt Collection. DNA sample was double digested with restriction enzymes EcoRI and MseI, and the fragments were linked to adapters. Selective amplification reactions were performed using five primer combinations and the amplified fragments were separated by gel electrophoresis on an ABI377 sequencer. Genetic distances were obtained by the complement of the Jaccard coefficient and the groups were performed by the UPGMA method. Five primer combinations generated 495 scorable fragments and 483 were found to be polymorphic. Out of 26 subspecies, strains 344 and T09 (B. thuringiensis subsp. tolworthi) showed the highest similarity (15%), while isolates HD3 B. thuringiensis subsp finitimus and T24 B. thuringiensis subsp neoleonensis were the most genetically distant (92%). B. thuringiensis isolates with no subspecies identification, found in samples from Goiás State showed higher similarity forming a group with an average distance of 6%, and the closest s... Mostrar Tudo |
|
Palavras-Chave: |
Bacillus thruringiensis; Diversidade genética. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02280naa a2200169 a 4500
001 1987279
005 2017-05-24
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1186/2193-1801-3-256$2DOI
100 1 $aVALICENTE, F. H.
245 $afAFLP analysis of Brazilian Bacillus thuringiensis isolates.$h[electronic resource]
260 $c2014
520 $aA total of 65 Bacillus thuringiensis (Bt) isolates were subjected to analysis of genetic relationship using fAFLP (fluorescent Fragment Length Polymorphism), in order to determine the genetic diversity within a group of Bt strains. 26 strains from different subspecies were identified as it follows: 9 kindly provided by the USDA (United States Department of Agriculture), 9 kindly provided by the Institute Pasteur and eight from Embrapa Maize and Sorghum Bt Collection, and 39 strains with no subspecies information also from Embrapa’s Bt Collection. DNA sample was double digested with restriction enzymes EcoRI and MseI, and the fragments were linked to adapters. Selective amplification reactions were performed using five primer combinations and the amplified fragments were separated by gel electrophoresis on an ABI377 sequencer. Genetic distances were obtained by the complement of the Jaccard coefficient and the groups were performed by the UPGMA method. Five primer combinations generated 495 scorable fragments and 483 were found to be polymorphic. Out of 26 subspecies, strains 344 and T09 (B. thuringiensis subsp. tolworthi) showed the highest similarity (15%), while isolates HD3 B. thuringiensis subsp finitimus and T24 B. thuringiensis subsp neoleonensis were the most genetically distant (92%). B. thuringiensis isolates with no subspecies identification, found in samples from Goiás State showed higher similarity forming a group with an average distance of 6%, and the closest subspecies to this group was B. thuringiensis subsp thuringiensis (HD2) with 52% of similarity. This similarity may be due to the fact that these organism exchange genetic material by conjugation, and it is relatively common to have evolutionary characteristics of their ancestors.
653 $aBacillus thruringiensis
653 $aDiversidade genética
700 1 $aSILVA, R. B. da
773 $tSpringerPlus$gv. 3, n. 256, 2014.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Meio-Norte. |
|
Data corrente: |
22/08/2022 |
|
Data da última atualização: |
12/12/2023 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
BARROS, E. K. do C.; ROCHA, M. de M.; SILVA, K. J. D. e. |
|
Afiliação: |
ELYNNE KRYSLLEN DO CARMO BARROS, PPGAN/ UFPI; MAURISRAEL DE MOURA ROCHA, CPAMN; KAESEL JACKSON DAMASCENO E SILVA, CPAMN. |
|
Título: |
Teores de proteínas em diferentes classes/subclasses comerciais de feijão-caupi. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA MEIO-NORTE, 5., 2019,Teresina, PI. Anais... Teresina: Embrapa Meio-Norte, 2022. 96 p. (Embrapa Meio-Norte. Documentos, 285). |
|
Páginas: |
p. 89. |
|
Idioma: |
Português |
|
Conteúdo: |
Neste trabalho, foi determinado o conteúdo proteico de 24 genótipos de feijão-caupi cultivados em Teresina, PI, no período de setembro a dezembro de 2018. A análise do teor de proteínas foi baseada na determinação de nitrogênio pelo método de Kjeldahl, utilizando-se o fator de conversão de 6,25. Realizaram-se análises de variância e as médias foram agrupadas pelo teste de Tocher. |
|
Palavras-Chave: |
Qualidade nutricional; Valor proteico. |
|
Thesagro: |
Vigna Unguiculata. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/239248/1/TeoresProteinasDiferentesClassesSubclassesFeijaoCaupiAnaisVJCCPAMNSet2019.pdf
|
|
Marc: |
LEADER 01075nam a2200181 a 4500
001 2145640
005 2023-12-12
008 2022 bl uuuu u00u1 u #d
100 1 $aBARROS, E. K. do C.
245 $aTeores de proteínas em diferentes classes/subclasses comerciais de feijão-caupi.$h[electronic resource]
260 $aIn: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA MEIO-NORTE, 5., 2019,Teresina, PI. Anais... Teresina: Embrapa Meio-Norte, 2022. 96 p. (Embrapa Meio-Norte. Documentos$c2022
300 $ap. 89.
520 $aNeste trabalho, foi determinado o conteúdo proteico de 24 genótipos de feijão-caupi cultivados em Teresina, PI, no período de setembro a dezembro de 2018. A análise do teor de proteínas foi baseada na determinação de nitrogênio pelo método de Kjeldahl, utilizando-se o fator de conversão de 6,25. Realizaram-se análises de variância e as médias foram agrupadas pelo teste de Tocher.
650 $aVigna Unguiculata
653 $aQualidade nutricional
653 $aValor proteico
700 1 $aROCHA, M. de M.
700 1 $aSILVA, K. J. D. e
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio-Norte (CPAMN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
