| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
22/01/2020 |
|
Data da última atualização: |
23/01/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
ALFRADIQUE, V. A. P.; OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; CÔRTES, L. R.; BRANDÃO, F. Z. |
|
Afiliação: |
VIVIAN ANGÉLICO PEREIRA ALFRADIQUE, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; CAROLINA CERQUEIRA SARMENTO OLIVARES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JOANNA MARIA GONÇALVES DE SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; MÁRIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; HELENA FABIANA REIS DE ALMEIDA SARAIVA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; LUANA RANGEL CÔRTES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil. |
|
Título: |
Mini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 47, Pub. 1707, p. 1-8, 2019. |
|
DOI: |
10.22456/1679-9216.98317 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Background: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, mini-Percoll, 1.5 h, 3 h, 6 h and 18 h. In comparison with post-thawing analysis, ovine species demonstrated a reduction (P < 0.05) in most of the sperm motility parameters after mini-Percoll. Conversely, ovine samples presented the highest (P < 0.05) rate of acrosome-reacted cells after mini-Percoll. Heparin supplementation did not affect most of the parameters evaluated (P > 0.05). In caprine and bovine species, a lower (P < 0.05) rate of sperm agglutination was observed in the presence of heparin at 18 h of incubation. In the absence of heparin, ovine samples showed a higher (P < 0.05) agglutination rate compared to the bovine species after long incubation period. Discussion: The present study compared sperm parameters (sperm kinematics, agglutination rate and capacitation status) of ruminant frozen-thawed sperm after mini-Percoll selection followed by in vitro incubation with or without heparin supplementation. In this study, it was observed the same rate of capacitated cells after the sperm selection (min-Percoll) between ruminant species. This indicate that the capacitation process occurs similarly between ruminant species, refuting the first hypothesis of this study. The presence of heparin did not influence the capacitation status of ruminant frozen-thawed sperm after mini-Percoll selection, it demonstrates that the second hypothesis was supported by this study making more economic and practical the use of ruminant frozen-thawed semen. The absence of heparin in the incubation medium did not harmed the capacitation status and sperm agglutination of ruminant frozen-thawed sperm. This supported the third hypothesis of the current study and indicate that the use of mini-Percoll technique regardless the presence of heparin could be a useful alternative for the preparation of ruminant frozen-thawed sperm. In conclusion, the capacitation status of ruminant frozen-thawed sperm is similar whether or not heparin is present after the mini-Percoll technique. MenosBackground: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, ... Mostrar Tudo |
|
Palavras-Chave: |
Billygoats; CTC. |
|
Thesagro: |
Bode; Bovino; Caprino; Carneiro; Espermatozóide; Heparina; Ovino; Reprodução Animal; Sêmen. |
|
Thesaurus Nal: |
Animal reproduction; Assisted reproductive technologies; Bulls; Cattle; Goats; Heparin; Rams; Sheep; Sperm capacitation. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/209450/1/cnpc-2019-Mini-Percoll.pdf
|
|
Marc: |
LEADER 04821naa a2200469 a 4500
001 2119218
005 2020-01-23
008 2019 bl uuuu u00u1 u #d
024 7 $a10.22456/1679-9216.98317$2DOI
100 1 $aALFRADIQUE, V. A. P.
245 $aMini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin.$h[electronic resource]
260 $c2019
520 $aBackground: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, mini-Percoll, 1.5 h, 3 h, 6 h and 18 h. In comparison with post-thawing analysis, ovine species demonstrated a reduction (P < 0.05) in most of the sperm motility parameters after mini-Percoll. Conversely, ovine samples presented the highest (P < 0.05) rate of acrosome-reacted cells after mini-Percoll. Heparin supplementation did not affect most of the parameters evaluated (P > 0.05). In caprine and bovine species, a lower (P < 0.05) rate of sperm agglutination was observed in the presence of heparin at 18 h of incubation. In the absence of heparin, ovine samples showed a higher (P < 0.05) agglutination rate compared to the bovine species after long incubation period. Discussion: The present study compared sperm parameters (sperm kinematics, agglutination rate and capacitation status) of ruminant frozen-thawed sperm after mini-Percoll selection followed by in vitro incubation with or without heparin supplementation. In this study, it was observed the same rate of capacitated cells after the sperm selection (min-Percoll) between ruminant species. This indicate that the capacitation process occurs similarly between ruminant species, refuting the first hypothesis of this study. The presence of heparin did not influence the capacitation status of ruminant frozen-thawed sperm after mini-Percoll selection, it demonstrates that the second hypothesis was supported by this study making more economic and practical the use of ruminant frozen-thawed semen. The absence of heparin in the incubation medium did not harmed the capacitation status and sperm agglutination of ruminant frozen-thawed sperm. This supported the third hypothesis of the current study and indicate that the use of mini-Percoll technique regardless the presence of heparin could be a useful alternative for the preparation of ruminant frozen-thawed sperm. In conclusion, the capacitation status of ruminant frozen-thawed sperm is similar whether or not heparin is present after the mini-Percoll technique.
650 $aAnimal reproduction
650 $aAssisted reproductive technologies
650 $aBulls
650 $aCattle
650 $aGoats
650 $aHeparin
650 $aRams
650 $aSheep
650 $aSperm capacitation
650 $aBode
650 $aBovino
650 $aCaprino
650 $aCarneiro
650 $aEspermatozóide
650 $aHeparina
650 $aOvino
650 $aReprodução Animal
650 $aSêmen
653 $aBillygoats
653 $aCTC
700 1 $aOLIVARES, C. C. S.
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aFONSECA, J. F. da
700 1 $aBATISTA, R. I. T. P.
700 1 $aBALARO, M. F. A.
700 1 $aSARAIVA, H. F. R. de A.
700 1 $aCÔRTES, L. R.
700 1 $aBRANDÃO, F. Z.
773 $tActa Scientiae Veterinariae$gv. 47, Pub. 1707, p. 1-8, 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 43 | |
| 21. |  | SILVA, A. G. DA; DINIZ, B. L. M. T.; CAVALCANTE, A. C. P.; DINIZ NETO, M. A.; SILVA, I. DE F. DA; SILVA, J. C. A. da. Produção do algodão colorido cv. topázio e brs 8h submetido a diferentes coberturas no solo. In: CONGRESSO BRASILEIRO DE MAMONA, 6.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 3., 2014, Fortaleza. Energia e segurança alimentar na agricultura familiar: anais. Campina Grande, PB: Embrapa Algodão, 2014. p. 67| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
|    |
| 25. | | VALE, D. G.; GUIMARAES, F. M.; OLIVEIRA, G. dos S.; CARDOSO, G. D.; ALVES, I.; SILVA, J. C. A. da; CARVALHO, L. P. de; SILVA, O. R. R. F.; CARTAXO, W. V. Algodão colorido : "tecnologia Embrapa para a geração de emprego e renda na agricultura familiar do Brasil". Campina Grande : Embrapa Algodão, 2011. 1 folder.| Tipo: Folder/Folheto/Cartilha |
| Biblioteca(s): Embrapa Algodão. |
| |
| 26. | | VALE, D. G.; BLOCH, D.; ALBUQUERQUE, F. A. de; SANTIAGO, F. S.; GUIMARAES, F. M.; ALVES, I.; SILVA, J. C. A. da; ARRIEL, N. H. C.; LIMA, P. J. B. FERREIRA. Algodão em consórcios agroecológicos. Campina Grande: Embrapa Algodão, 2009. 1 folder| Tipo: Folder/Folheto/Cartilha |
| Biblioteca(s): Embrapa Algodão. |
| |
| 27. | | VALE, D. G.; BLOCH, D.; ALBUQUERQUE, F. A. de; SANTIAGO, F. S.; GUIMARAES, F. M.; ALVES, I.; SILVA, J. C. A. da; ARRIEL, N. H. C.; LIMA, P. J. B. FERREIRA; SILVA, V. A. da. Algodão em consórcios agroecológicos. 3. ed. Campina Grande: Embrapa Algodão, 2011. 1 folder| Biblioteca(s): Embrapa Algodão. |
| |
| 28. | | OLIVEIRA, J. M. C. de; ARAÚJO, J. M. de; SOUSA, M. F. de; ALBUQUERQUE, F. A. de; CARTAXO, W. V.; SILVA, J. C. A. da; VALE, D. G.; CARDOSO, G. D. de; ALVES, I.; CAVALCANTI, M. L. F. Avaliação de unidades demonstrativas de algodoeiro herbáceo de sequeiro no Estado da Paraíba - 2006. In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. Anais... Uberlândia, 2007. p. 1-4| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Algodão. |
| |
| 29. | | SILVA, J. C. A. da; SANTANA, J. C. F. de; CARTAXO, W. V.; VALE, D. G.; VALE JUNIOR, F. de M.; OLIVEIRA, J. M. C. de; ALVES, I.; SOUSA, M. F. de; ALBUQUERQUE, F. A. de; CARDOSO, G. D. Avaliação de unidades de teste e demonstração: qualidade agronômica e tecnológica de cinco cultivares de algodão testadas no município de Quixadá - CE, safra 2002/2003. In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. Anais... Uberlândia, 2007. p. 1-4 1 CD-ROM| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Algodão. |
| |
| 30. | | FREITAS, J. G.; ALBUQUERQUE, F. A. de; SILVA, J. C. A. da; MOTA, J. R.; SILVA, G. A. da; SILVA, C. F. L. da; SILVA, F. de S.; SILVA, J. M. da. Avaliação de quatro densidades populacional cultivar BRS ENERGIA em regime de sequeiro em Irecê-BA. CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão, 2012. p. 267| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
| |
| 31. | | VALE, D. G.; GUIMARAES, F. M.; OLIVEIRA, G. dos S.; CARDOSO, G. D.; ALVES, I.; SILVA, J. C. A. da; SILVA, O. R. R. F.; CARTAXO, W. V.; CARVALHO, L. P. de. BRS Topázio. Campina Grande: Embrapa Algodão, 2011.| Tipo: Folder/Folheto/Cartilha |
| Biblioteca(s): Embrapa Algodão. |
| |
| 34. | | SILVA, J. C. A. da; SANTANA, J. C. F. de; CARTAXO, W. V.; VALE, D. G.; ALVES, I.; SOUSA, M. F. de; OLIVEIRA, J. M. C. de; ALBUQUERQUE, F. A. de; CARDOSO, G. D. Desempenho de UTDs de algodão: avaliação agronômica e tecnológica da fibra da cultivar BRS 187-8H provenientes do Agreste Paraibano safra 2003. In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. Anais... Uberlândia, 2007. p. 1-4 1 CD-ROM| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Algodão. |
| |
| 35. | | SILVA, O. R. R. F. da; FREIRE, E. C.; CARTAXO, W. V.; SILVA, J. C. A. da; SILVA, C. A. P. da; ARAÚJO, M. R. T. de; MONTEIRO, R. C. C.; SILVA, A. V. da; CAVALCANTE, A. J. de A. Projeto Massabielle Campina Grande: Embrapa Algodão, 2003. não paginado Folder| Biblioteca(s): Embrapa Algodão. |
| |
| 36. | | FREITAS, J. G.; SILVA, J. C. A. da; MILANI, M.; ALVES. I.; VALLE, D. G.; AMORIM, R. S.; CARDOSO, G. D.; OLIVEIRA, J. M. C. de; CARTAXO, W. V.; GUIMARÃES, F. M.; MOTA, J. R.; SILVA, G. A. da. RECOMENDAÇÕES técnicas para o cultivo da mamona na Região de Irecê. Campina Grande: Embrapa Algodão, 2008. 1 folder| Tipo: Folder/Folheto/Cartilha |
| Biblioteca(s): Embrapa Algodão. |
|  |
| 37. | | SILVA, J. C. A. da; CARTAXO, W. V.; SILVA, O. R. R. F. da; MOTA, V. de J.; VANDERLEI, J. C.; GOTTENS, W. H.; PEIXOTO, P. C. da C.; FIÚZA, O. P. Núcleos avançados de produção associativa de algodão, na agricultura familiar no Estado de Goiás. Campina Grande: Embrapa Algodão, 2006. não paginado Folder| Tipo: Folder/Folheto/Cartilha |
| Biblioteca(s): Embrapa Algodão. |
| |
| 38. | | FREITAS, J. G.; VALE, D. G.; SILVA, J. C. A. da; ALBUQUERQUE, F. A. de; VALE JUNIOR, F. de M.; AGENOR, A. A.; SOUSA, R. V. e; OLIVEIRA, C. A. L. de; ALVES, I.; GUIMARÃES, F. M.; CARDOSO, G. D.; SOUSA, M. F. de; OLIVEIRA, J. M. C. de; CARTAXO, W. V.; COLUMBIANO, V. de S. Desempenho das unidades demonstrativas de algodoeiro herbáceo de sequeiro instaladas no Estado do Ceará no período de 2002-2004. In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. Anais... Uberlândia, 2007. p. 1-5 1 CD-ROM Trabalhos| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Algodão. |
| |
| 39. | | SILVA, J. C. A. da; VALE, D. G.; ALBUQUERQUE, F. A. de; VALE JUNIOR, F. de M.; AGENOR, A. A.; SOUSA, R. V. e; OLIVEIRA, C. A. L. de; ALVES, I.; GUIMARÃES, F. M.; CARDOSO, G. D.; SOUSA, M. F. de; OLIVEIRA, J. M. C. de; CARTAXO, W. V. Resultados técnico-econômicos de unidades de teste e de demonstração (UTDs) de algodão BRS 8H instaladas em seis municípios do estado do Ceará no ano de 2003. In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. Anais... Uberlândia, 2007. p. 1-4 1 CD-ROM Trabalhos| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Algodão. |
| |
| 40. | | VALE, D. G.; SILVA, J. C. A. da; ALBUQUERQUE, F. A. de; VALE JUNIOR, F. de M.; AGENOR, A. A.; SOUSA, R. V. e; OLIVEIRA, C. A. L. de; ALVES, I.; GUIMARÃES, F. M.; CARDOSO, G. D.; SOUSA, M. F. de; OLIVEIRA, J. M. C. de; CARTAXO, W. V. Resultados técnicos e econômicos de unidades de teste e demonstração (UTDs) de algodão BRS 201 em seis municípios do estado do Ceará, safra 2004. In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. Anais... Uberlândia, 2007. p. 1-4 1 CD-ROM Trabalhos| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Algodão. |
| |
| Registros recuperados : 43 | |
|
| Expressão de busca inválida. Verifique!!! |
|
|
