|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
02/03/2021 |
Data da última atualização: |
06/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CUNHA, A. F.; ANDRADE, H. M.; SOUZA, F. N.; FIALHO JUNIOR, L. C.; ROSA, D. L. S. O.; SANCHEZ, E. M. R.; GIDLUND, M.; GOTO, H.; BRITO, M. A. V. P.; GUIMARÃES, A. S.; LAGE, A. P.; REIS, L. C.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. |
Afiliação: |
A. F. Cunha, UFMG; H. M. Andrade, UFMG; F. N. Souza, UFMG; L. C. Fialho Júnior, UFMG; D. L. S. O. Rosa, UFMG; E. M. Ramos Sanchez, USP; M. Gidlund, USP; H. Goto, USP; M. A. V. P. Brito, Pesquisadora aposentada da Embrapa Gado de Leite.; ALESSANDRO DE SA GUIMARAES, CNPGL; A. P. Lage, UFMG; L. C. Reis, USP; A. M. M. P. Della Libera, USP; M. B. Heinemann, USP; M. M. O. P. Cerqueira, UFMG. |
Título: |
Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Journal of Dairy Science, v. 103, n. 5, p. 4588-4605, 2020. |
Idioma: |
Inglês |
Conteúdo: |
ABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets. MenosABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among ani... Mostrar Tudo |
Palavras-Chave: |
Immunoproteomics; Intramammary infection; Serological proteome analysis. |
Thesagro: |
Staphylococcus Aureus. |
Thesaurus Nal: |
Vaccines. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02811naa a2200349 a 4500 001 2130440 005 2024-02-06 008 2020 bl uuuu u00u1 u #d 100 1 $aCUNHA, A. F. 245 $aComparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine.$h[electronic resource] 260 $c2020 520 $aABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets. 650 $aVaccines 650 $aStaphylococcus Aureus 653 $aImmunoproteomics 653 $aIntramammary infection 653 $aSerological proteome analysis 700 1 $aANDRADE, H. M. 700 1 $aSOUZA, F. N. 700 1 $aFIALHO JUNIOR, L. C. 700 1 $aROSA, D. L. S. O. 700 1 $aSANCHEZ, E. M. R. 700 1 $aGIDLUND, M. 700 1 $aGOTO, H. 700 1 $aBRITO, M. A. V. P. 700 1 $aGUIMARÃES, A. S. 700 1 $aLAGE, A. P. 700 1 $aREIS, L. C. 700 1 $aLIBERA, A. M. M. P. D. 700 1 $aHEINEMANN, M. B. 700 1 $aCERQUEIRA, M. M. O. P 773 $tJournal of Dairy Science$gv. 103, n. 5, p. 4588-4605, 2020.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
01/11/2016 |
Data da última atualização: |
27/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
PIMENTA, M. R.; SILVA, P. A.; MENDES, G. C.; ALVES, J. R.; CAETANO, H. D. N.; MACHADO, J. P. B.; BRUSTOLINI, O. J. B.; CARPINETTI, P. A.; MELO, B. P.; SILVA, J. C. F.; ROSADO, G. L.; FERREIRA, M. F. S.; DAL-BIANCO, M.; PICOLI, E. A. de T.; ARAGAO, F. J. L.; RAMOS, H. J. O.; FONTES, E. P. B. |
Afiliação: |
MAIANA REIS PIMENTA, UFV; PRISCILA ALVES SILVA, UFV; GISELLE CAMARGO MENDES, UFV; JANAÍNA ROBERTA ALVES, UFV; HANNA DURSO NEVES CAETANO, UFV; JOAO PAULO BATISTA MACHADO, UFV; OTAVIO JOSÉ BERNARDES BRUSTOLINI, UFV; PAOLA AVELAR CARPINETTI, UFV; BRUNO PAES MELO, UFV; JOSÉ CLEYDSON FERREIRA SILVA, UFV; GUSTAVO LEÃO ROSADO, UFV; MÁRCIA FLORES SILVA FERREIRA, UFV; MAXIMILLIR DAL-BIANCO, UFV; EDGARD AUGUSTO DE TOLEDO PICOLI, UFV; FRANCISCO JOSE LIMA ARAGAO, Cenargen; HUMBERTO JOSUÉ OLIVEIRA RAMOS, UFV; ELIZABETH PACHECO BATISTA FONTES, UFV. |
Título: |
The Stress-Induced Soybean NAC transcription factor GmNAC81 plays a positive role in developmentally programmed leaf senescence. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Plant and Cell Physiology, v. 57, n. 5, p. 1098-1114, 2016. |
DOI: |
10.1093/pcp/pcw059 |
Idioma: |
Inglês |
Palavras-Chave: |
Developmental regulation; GmNAC81; Leaf senescence; NAC family; Transcription factor. |
Thesagro: |
Glycine Max. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01164naa a2200385 a 4500 001 2055743 005 2023-03-27 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1093/pcp/pcw059$2DOI 100 1 $aPIMENTA, M. R. 245 $aThe Stress-Induced Soybean NAC transcription factor GmNAC81 plays a positive role in developmentally programmed leaf senescence.$h[electronic resource] 260 $c2016 650 $aGlycine Max 653 $aDevelopmental regulation 653 $aGmNAC81 653 $aLeaf senescence 653 $aNAC family 653 $aTranscription factor 700 1 $aSILVA, P. A. 700 1 $aMENDES, G. C. 700 1 $aALVES, J. R. 700 1 $aCAETANO, H. D. N. 700 1 $aMACHADO, J. P. B. 700 1 $aBRUSTOLINI, O. J. B. 700 1 $aCARPINETTI, P. A. 700 1 $aMELO, B. P. 700 1 $aSILVA, J. C. F. 700 1 $aROSADO, G. L. 700 1 $aFERREIRA, M. F. S. 700 1 $aDAL-BIANCO, M. 700 1 $aPICOLI, E. A. de T. 700 1 $aARAGAO, F. J. L. 700 1 $aRAMOS, H. J. O. 700 1 $aFONTES, E. P. B. 773 $tPlant and Cell Physiology$gv. 57, n. 5, p. 1098-1114, 2016.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|