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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
04/10/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
POPPL, A. G.; VALLE, S. C.; GONZÁLEZ, F. H. D.; KUCHARSKI, L. C.; SILVA, R. S. M. da. |
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Afiliação: |
ÁLAN G. POPPL, UFRGS; SANDRA C. VALLE, UFRGS; FÉLIX H. D. GONZÁLEZ, UFRGS; LUIZ C. KUCHARSKI, UFRGS; ROSELIS S. M. DA SILVA, UFRGS. |
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Título: |
Insulin binding characteristics in canine muscle tissue: effects of the estrous cycle phases. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 8, p. 761-766, ago. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Hormonal fluctuations during the different estrous cycle are a well-recognized cause of insulin resistance in bitches, and little is known about insulin receptor binding or post-binding defects associated with insulin resistance in dogs. To evaluate insulin binding characteristics in muscle tissue of bitches during the estrous cycle, 17 owned bitches were used in the study (six in anestrus, five in estrus, and six in diestrus). An intravenous glucose tolerance test (IVGTT) was performed in all patients by means of injection of 1mL/kg of a glucose 50% solution (500mg/kg), with blood sample collection for glucose determination at 0, 3, 5, 7, 15, 30, 45 and 60 minutes after glucose infusion. Muscle samples, taken after spaying surgery, were immediately frozen in liquid nitrogen and then stored at -80 ºC until the membranes were prepared by sequential centrifugation after being homogenized. For binding studies, membranes were incubated in the presence of 20,000cpm of human 125I-insulin and in increasing concentrations of unlabeled human regular insulin for cold saturation. The IVGTT showed no differences among bitches during the estrous cycle regarding baseline glycemia or glycemic response after glucose infusion. Two insulin binding sites - high-affinity and low-affinity ones - were detected by Scatchard analysis, and significant statistical differences were observed in the dissociation constant (Kd1) and maximum binding capacity (Bmax1) of the high-affinity binding sites. The Kd1 for the anestrus group (6.54±2.77nM/mg of protein) was smaller (P<0.001) than for the estrus (28.54±6.94nM/mg of protein) and diestrus (15.56±3.88nM/mg of protein) groups. Bmax1 in the estrus (0.83±0.42nM/mg of protein) and diestrus (1.24±0.24nM/mg of protein) groups were also higher (P<0.001) than the values observed in anestrus (0.35±0.06nM/mg of protein). These results indicate modulation of insulin binding characteristics during different phases of the estrous cycle in dogs, showing that muscle insulin binding affinity for its receptor is reduced during estrus and diestrus. However, this poor hormone-receptor affinity is compensated for by a greater total binding capacity, once there is no difference in patients? glycemic response after an intravenous glucose load. MenosHormonal fluctuations during the different estrous cycle are a well-recognized cause of insulin resistance in bitches, and little is known about insulin receptor binding or post-binding defects associated with insulin resistance in dogs. To evaluate insulin binding characteristics in muscle tissue of bitches during the estrous cycle, 17 owned bitches were used in the study (six in anestrus, five in estrus, and six in diestrus). An intravenous glucose tolerance test (IVGTT) was performed in all patients by means of injection of 1mL/kg of a glucose 50% solution (500mg/kg), with blood sample collection for glucose determination at 0, 3, 5, 7, 15, 30, 45 and 60 minutes after glucose infusion. Muscle samples, taken after spaying surgery, were immediately frozen in liquid nitrogen and then stored at -80 ºC until the membranes were prepared by sequential centrifugation after being homogenized. For binding studies, membranes were incubated in the presence of 20,000cpm of human 125I-insulin and in increasing concentrations of unlabeled human regular insulin for cold saturation. The IVGTT showed no differences among bitches during the estrous cycle regarding baseline glycemia or glycemic response after glucose infusion. Two insulin binding sites - high-affinity and low-affinity ones - were detected by Scatchard analysis, and significant statistical differences were observed in the dissociation constant (Kd1) and maximum binding capacity (Bmax1) of the high-affinity binding sites. The ... Mostrar Tudo |
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Palavras-Chave: |
Insulin sensitivity; Receptor tirosina quinase; Resistência insulinica; Tyrosine kinase receptors. |
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Thesagro: |
Cão; Ciclo estral; Musculo. |
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Thesaurus Nal: |
diabetes mellitus; Dogs; Estrous cycle; Insulin resistance; Muscle tissues. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148294/1/Insulin-binding-characteristics.pdf
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Marc: |
LEADER 03212naa a2200313 a 4500
001 2054054
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aPOPPL, A. G.
245 $aInsulin binding characteristics in canine muscle tissue$beffects of the estrous cycle phases.
260 $c2016
520 $aHormonal fluctuations during the different estrous cycle are a well-recognized cause of insulin resistance in bitches, and little is known about insulin receptor binding or post-binding defects associated with insulin resistance in dogs. To evaluate insulin binding characteristics in muscle tissue of bitches during the estrous cycle, 17 owned bitches were used in the study (six in anestrus, five in estrus, and six in diestrus). An intravenous glucose tolerance test (IVGTT) was performed in all patients by means of injection of 1mL/kg of a glucose 50% solution (500mg/kg), with blood sample collection for glucose determination at 0, 3, 5, 7, 15, 30, 45 and 60 minutes after glucose infusion. Muscle samples, taken after spaying surgery, were immediately frozen in liquid nitrogen and then stored at -80 ºC until the membranes were prepared by sequential centrifugation after being homogenized. For binding studies, membranes were incubated in the presence of 20,000cpm of human 125I-insulin and in increasing concentrations of unlabeled human regular insulin for cold saturation. The IVGTT showed no differences among bitches during the estrous cycle regarding baseline glycemia or glycemic response after glucose infusion. Two insulin binding sites - high-affinity and low-affinity ones - were detected by Scatchard analysis, and significant statistical differences were observed in the dissociation constant (Kd1) and maximum binding capacity (Bmax1) of the high-affinity binding sites. The Kd1 for the anestrus group (6.54±2.77nM/mg of protein) was smaller (P<0.001) than for the estrus (28.54±6.94nM/mg of protein) and diestrus (15.56±3.88nM/mg of protein) groups. Bmax1 in the estrus (0.83±0.42nM/mg of protein) and diestrus (1.24±0.24nM/mg of protein) groups were also higher (P<0.001) than the values observed in anestrus (0.35±0.06nM/mg of protein). These results indicate modulation of insulin binding characteristics during different phases of the estrous cycle in dogs, showing that muscle insulin binding affinity for its receptor is reduced during estrus and diestrus. However, this poor hormone-receptor affinity is compensated for by a greater total binding capacity, once there is no difference in patients? glycemic response after an intravenous glucose load.
650 $adiabetes mellitus
650 $aDogs
650 $aEstrous cycle
650 $aInsulin resistance
650 $aMuscle tissues
650 $aCão
650 $aCiclo estral
650 $aMusculo
653 $aInsulin sensitivity
653 $aReceptor tirosina quinase
653 $aResistência insulinica
653 $aTyrosine kinase receptors
700 1 $aVALLE, S. C.
700 1 $aGONZÁLEZ, F. H. D.
700 1 $aKUCHARSKI, L. C.
700 1 $aSILVA, R. S. M. da
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 8, p. 761-766, ago. 2016.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Pecuária Sudeste. |
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Data corrente: |
02/12/2014 |
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Data da última atualização: |
10/06/2022 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SILVA, J. A. R. da; SANTOS, N. de F. A. dos; ARAÚJO, A. A. de; LOURENÇO JUNIOR, J. de B.; GARCIA, A. R.; CORDEIRO, C. da C.; COSTA, T. S. A. da; JUCÁ, A. C. C. |
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Afiliação: |
Jamile Andréa Rodrigues da Silva, UFRA; Núbia de Fátima Alves dos Santos, UFRA; Airton Alencar de Araújo, UECE; José de Brito Lourenço Júnior, UEPA; ALEXANDRE ROSSETTO GARCIA, CPATU; Camila da Conceição Cordeiro, GRADUANDA UFRA; Thays Syntya Antunes da Costa, GRADUANDA UFRA; Ana Carolina Cavalcante Jucá, GRADUANDA UFRA. |
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Título: |
Avaliação de dois diferentes sistemas de criação para o conforto térmico de búfalas Murrah, criadas na Amazônia Oriental. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 24., 2014, Vitória. A zootecnia fazendo o Brasil crescer: anais... Vitória: Universidade Federal do Espírito Santo, 2014. |
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Idioma: |
Português |
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Conteúdo: |
Esta pesquisa objetivou avaliar o conforto térmico de búfalas Murrah, em dois diferentes sistemas de criação: Sistemas Tradicional (ST) e Silvipastoril (SSP), na Amazônia Oriental. O experimento foi realizado na Embrapa Amazônia Oriental, em tipo climático Afi, segundo Köppen. O período experimental foi de doze meses, em dois turnos (manhã e tarde). Os animais foram inseridos no ST (n=10), composto por três piquetes, sem acesso à água para banho e sombreamento, e SSP (n=10), com sombreamento da espécie florestal Racosperma mangium. Foram aferidas variáveis meteorológicas e fisiológicas, e calculado o Índice de Conforto de Benezra (ICB), para os dois sistemas de criação, e comparados pelo Teste t (P<0,05). A temperatura retal (TR) esteve acima da faixa de variação normal para bubalinos. A frequência respiratória (FR), no turno da tarde, para os dois sistemas de criação, estavam acima dos níveis considerados normais para búfalos. Os ICBs no ST ficaram na faixa de 2,25 ± 0,18 a 2,50 ± 0,32 e no SSP entre 2,22 ± 0,19 e 2,41 ± 0,27 (P>0,05). Por disponibilizar sombreamento, o SSP propiciou melhores condições de conforto térmico para as búfalas. |
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Palavras-Chave: |
Bubalino; Conforto térmico; Raça Murrah. |
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Thesagro: |
Bioclimatologia. |
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Thesaurus NAL: |
Amazonia. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/112769/1/R1236-1-374436174.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/115514/1/PROCI-2014.00209.pdf
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Marc: |
LEADER 02089nam a2200253 a 4500
001 2001263
005 2022-06-10
008 2014 bl uuuu u00u1 u #d
100 1 $aSILVA, J. A. R. da
245 $aAvaliação de dois diferentes sistemas de criação para o conforto térmico de búfalas Murrah, criadas na Amazônia Oriental.
260 $aIn: CONGRESSO BRASILEIRO DE ZOOTECNIA, 24., 2014, Vitória. A zootecnia fazendo o Brasil crescer: anais... Vitória: Universidade Federal do Espírito Santo$c2014
520 $aEsta pesquisa objetivou avaliar o conforto térmico de búfalas Murrah, em dois diferentes sistemas de criação: Sistemas Tradicional (ST) e Silvipastoril (SSP), na Amazônia Oriental. O experimento foi realizado na Embrapa Amazônia Oriental, em tipo climático Afi, segundo Köppen. O período experimental foi de doze meses, em dois turnos (manhã e tarde). Os animais foram inseridos no ST (n=10), composto por três piquetes, sem acesso à água para banho e sombreamento, e SSP (n=10), com sombreamento da espécie florestal Racosperma mangium. Foram aferidas variáveis meteorológicas e fisiológicas, e calculado o Índice de Conforto de Benezra (ICB), para os dois sistemas de criação, e comparados pelo Teste t (P<0,05). A temperatura retal (TR) esteve acima da faixa de variação normal para bubalinos. A frequência respiratória (FR), no turno da tarde, para os dois sistemas de criação, estavam acima dos níveis considerados normais para búfalos. Os ICBs no ST ficaram na faixa de 2,25 ± 0,18 a 2,50 ± 0,32 e no SSP entre 2,22 ± 0,19 e 2,41 ± 0,27 (P>0,05). Por disponibilizar sombreamento, o SSP propiciou melhores condições de conforto térmico para as búfalas.
650 $aAmazonia
650 $aBioclimatologia
653 $aBubalino
653 $aConforto térmico
653 $aRaça Murrah
700 1 $aSANTOS, N. de F. A. dos
700 1 $aARAÚJO, A. A. de
700 1 $aLOURENÇO JUNIOR, J. de B.
700 1 $aGARCIA, A. R.
700 1 $aCORDEIRO, C. da C.
700 1 $aCOSTA, T. S. A. da
700 1 $aJUCÁ, A. C. C.
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Embrapa Amazônia Oriental (CPATU) |
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