| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agrobiologia. |
|
Data corrente: |
12/04/1999 |
|
Data da última atualização: |
12/04/1999 |
|
Autoria: |
MARTINS, L. M. V.; XAVIER, G. R.; NEVES, Maria Cristina P.; RUMJANEK, Norma G. |
|
Título: |
Aluminio como elemento determinante na resistencia intrinseca a antibioticos em populacao de rizobio que nodula caupi (Vigna unguiculata (L.) Walp). |
|
Ano de publicação: |
1997 |
|
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE CIENCIA DO SOLO, 26., jul. 1997, Rio de Janeiro. Resumos... Rio de Janeiro: Sociedade Brasileira de Ciencia do Solo, 1997. Secao Tematica 3. 1 CD-ROM. |
|
Idioma: |
Português |
|
Notas: |
Mini-resumo impresso na pagina 128. |
|
Conteúdo: |
Uma colecao de rizobio de caupi, foi coletada de solos do Nordeste Brasileiro e avaliada quanto a resistencia intrinseca a oito antibioticos comerciais, de modo a correlaciona-la com fatores edafico-climaticos. Os niveis de aluminio no solo foram capazes de influenciar a distribuicao da populacao de rizobio. Isolados sensiveis a 5 antibioticos foram mais frequentes em solos isentos de aluminio. Nesse trabalho, sugere-se que a selecao de um inoculante deve levar em consideracao, nao somente seu potencial genetico, mas tambem a interacao deste com as condicoes edafico-climaticos. |
|
Palavras-Chave: |
Al; Resistance to chemicals. |
|
Thesagro: |
Alumínio; Antibiótico; Feijão de Corda; Resistência a Produtos Químicos; Rhizobium; Vigna Unguiculata. |
|
Thesaurus Nal: |
aluminum; antibiotics; cowpeas. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01630naa a2200301 a 4500
001 1621012
005 1999-04-12
008 1997 bl uuuu u00u1 u #d
100 1 $aMARTINS, L. M. V.
245 $aAluminio como elemento determinante na resistencia intrinseca a antibioticos em populacao de rizobio que nodula caupi (Vigna unguiculata (L.) Walp).
260 $c1997
500 $aMini-resumo impresso na pagina 128.
520 $aUma colecao de rizobio de caupi, foi coletada de solos do Nordeste Brasileiro e avaliada quanto a resistencia intrinseca a oito antibioticos comerciais, de modo a correlaciona-la com fatores edafico-climaticos. Os niveis de aluminio no solo foram capazes de influenciar a distribuicao da populacao de rizobio. Isolados sensiveis a 5 antibioticos foram mais frequentes em solos isentos de aluminio. Nesse trabalho, sugere-se que a selecao de um inoculante deve levar em consideracao, nao somente seu potencial genetico, mas tambem a interacao deste com as condicoes edafico-climaticos.
650 $aaluminum
650 $aantibiotics
650 $acowpeas
650 $aAlumínio
650 $aAntibiótico
650 $aFeijão de Corda
650 $aResistência a Produtos Químicos
650 $aRhizobium
650 $aVigna Unguiculata
653 $aAl
653 $aResistance to chemicals
700 1 $aXAVIER, G. R.
700 1 $aNEVES, Maria Cristina P.
700 1 $aRUMJANEK, Norma G.
773 $tIn: CONGRESSO BRASILEIRO DE CIENCIA DO SOLO, 26., jul. 1997, Rio de Janeiro. Resumos... Rio de Janeiro: Sociedade Brasileira de Ciencia do Solo, 1997. Secao Tematica 3. 1 CD-ROM.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
08/12/2011 |
|
Data da última atualização: |
05/02/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
SILVA, I. O. e; VASCONCELOS, R. B.; CAETANO, J. V. O.; GULART, L. V. M.; CAMARGO, L. S. de A.; BÁO, S. N.; SILVA, A. A. M. R. e. |
|
Afiliação: |
I. OLIVEIRA E SILVA, UNB; R. B. VASCONCELOS, UNB; J. V. O. CAETANO, UNB; L. V. M. GULART, UNB; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; S. N. BÁO, UNB; A. A. M. ROSA E SILVA, UNB. |
|
Título: |
Induction of reversible meiosis arrest of bovine oocytes using a two-step procedure under defined and nondefined conditions. |
|
Ano de publicação: |
2011 |
|
Fonte/Imprenta: |
Theriogenology, v. 75, n. 6, p. 1115-1124, 2011. |
|
DOI: |
https://doi.org/10.1016/j.theriogenology.2010.11.021 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The objective was to study the effect of a defined culture system, on nuclear and cytoplasmic maturation of bovine oocytes, using the two-step procedure of IVM to detect possible inhibition and subsequent resumption of meiosis arrest. In the first step, called the prematuration period (PMP), COCs were cultured in T1-non-defined medium (NDM), or T2-defined medium (DM), both for 24 h. In step 2, called the resumption period (RP), COCs were cultured in: NDM (T1); DM + NDM (T3); or DM+DM (T4) for 24 h in each medium. The NDM was composed of TCM-199 supplemented with FCS and FSH. The DM was composed of alpha-MEM supplemented with PVA, insulin, IGF-1, androstenedione, nonessential amino acids, transferrin, and sodium selenium. Oocytes from T2 had a lower (P < 0.05) rate of nuclear maturation (19.8%) than T1 oocytes (83.2%). Also, T2 COCs appeared to be in the process of cytoplasmic maturation, according to the distribution of organelles assessed by transmission electron microscopy (MET). These COCs had characteristics previously described as mature: erect microvilli on the plasmembrane, presence of cortical/evenly distributed mitochondria throughout the ooplasm, and presence of 50% aligned/cluster cortical granules. Immature characteristics such as small PvS, compact cumulus cells, and presence of 50% cortical granule clusters were also observed. The T1 COCs had only characteristics of maturation (P < 0.05). In step 2 (RP), meiosis arrest induced by DM was resumed after an additional 24 h of culture in NDM (T3) with 79.2% mature COCs, whereas in T4, meiosis arrest was maintained, resulting in almost 70% immature COCs (P < 0.05). At the end of RP, T3 COCs had the mature characteristics of mitochondria spread throughout the cytoplasm (P < 0.05), cumulus expansion, and alignment of cortical granules, whereas the T4 group had both immature and mature characteristics. We inferred that DM can be used in lieu of meiosis inhibitors and furthermore, it can provide extra time to study nuclear and cytoplasmic maturation synchrony of IVM. MenosThe objective was to study the effect of a defined culture system, on nuclear and cytoplasmic maturation of bovine oocytes, using the two-step procedure of IVM to detect possible inhibition and subsequent resumption of meiosis arrest. In the first step, called the prematuration period (PMP), COCs were cultured in T1-non-defined medium (NDM), or T2-defined medium (DM), both for 24 h. In step 2, called the resumption period (RP), COCs were cultured in: NDM (T1); DM + NDM (T3); or DM+DM (T4) for 24 h in each medium. The NDM was composed of TCM-199 supplemented with FCS and FSH. The DM was composed of alpha-MEM supplemented with PVA, insulin, IGF-1, androstenedione, nonessential amino acids, transferrin, and sodium selenium. Oocytes from T2 had a lower (P < 0.05) rate of nuclear maturation (19.8%) than T1 oocytes (83.2%). Also, T2 COCs appeared to be in the process of cytoplasmic maturation, according to the distribution of organelles assessed by transmission electron microscopy (MET). These COCs had characteristics previously described as mature: erect microvilli on the plasmembrane, presence of cortical/evenly distributed mitochondria throughout the ooplasm, and presence of 50% aligned/cluster cortical granules. Immature characteristics such as small PvS, compact cumulus cells, and presence of 50% cortical granule clusters were also observed. The T1 COCs had only characteristics of maturation (P < 0.05). In step 2 (RP), meiosis arrest induced by DM was resumed after an additio... Mostrar Tudo |
|
Palavras-Chave: |
Chemically defined medium; Cytoplasmic maturation; Nuclear maturation. |
|
Thesaurus NAL: |
Oocytes. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02902naa a2200253 a 4500
001 1909108
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2010.11.021$2DOI
100 1 $aSILVA, I. O. e
245 $aInduction of reversible meiosis arrest of bovine oocytes using a two-step procedure under defined and nondefined conditions.$h[electronic resource]
260 $c2011
520 $aThe objective was to study the effect of a defined culture system, on nuclear and cytoplasmic maturation of bovine oocytes, using the two-step procedure of IVM to detect possible inhibition and subsequent resumption of meiosis arrest. In the first step, called the prematuration period (PMP), COCs were cultured in T1-non-defined medium (NDM), or T2-defined medium (DM), both for 24 h. In step 2, called the resumption period (RP), COCs were cultured in: NDM (T1); DM + NDM (T3); or DM+DM (T4) for 24 h in each medium. The NDM was composed of TCM-199 supplemented with FCS and FSH. The DM was composed of alpha-MEM supplemented with PVA, insulin, IGF-1, androstenedione, nonessential amino acids, transferrin, and sodium selenium. Oocytes from T2 had a lower (P < 0.05) rate of nuclear maturation (19.8%) than T1 oocytes (83.2%). Also, T2 COCs appeared to be in the process of cytoplasmic maturation, according to the distribution of organelles assessed by transmission electron microscopy (MET). These COCs had characteristics previously described as mature: erect microvilli on the plasmembrane, presence of cortical/evenly distributed mitochondria throughout the ooplasm, and presence of 50% aligned/cluster cortical granules. Immature characteristics such as small PvS, compact cumulus cells, and presence of 50% cortical granule clusters were also observed. The T1 COCs had only characteristics of maturation (P < 0.05). In step 2 (RP), meiosis arrest induced by DM was resumed after an additional 24 h of culture in NDM (T3) with 79.2% mature COCs, whereas in T4, meiosis arrest was maintained, resulting in almost 70% immature COCs (P < 0.05). At the end of RP, T3 COCs had the mature characteristics of mitochondria spread throughout the cytoplasm (P < 0.05), cumulus expansion, and alignment of cortical granules, whereas the T4 group had both immature and mature characteristics. We inferred that DM can be used in lieu of meiosis inhibitors and furthermore, it can provide extra time to study nuclear and cytoplasmic maturation synchrony of IVM.
650 $aOocytes
653 $aChemically defined medium
653 $aCytoplasmic maturation
653 $aNuclear maturation
700 1 $aVASCONCELOS, R. B.
700 1 $aCAETANO, J. V. O.
700 1 $aGULART, L. V. M.
700 1 $aCAMARGO, L. S. de A.
700 1 $aBÁO, S. N.
700 1 $aSILVA, A. A. M. R. e
773 $tTheriogenology$gv. 75, n. 6, p. 1115-1124, 2011.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
