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Registros recuperados : 7 | |
1. | | PEREIRA, M. da C. P.; SOUZA, N. O. S.; FERREIRA, M. E.; REIS, M. I. C. C. dos; LIMA, B. P. de. Avaliação de características morfofisiológicas em uma coleção nuclear de arroz para tolerância à seca. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 8., 2015, Goiânia. O melhoramento de plantas, o futuro da agricultura e a soberania nacional: anais. Goiânia: UFG: SBMP, 2015. Resumo 348-259. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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2. | | LIMA, B. P. de; ALVES-FREITAS, D. M. T.; MELO, F. L. de; CARVALHO, R. de C. P.; FARIA, J. C. de; RIBEIRO, S. G. Characterization of a new whitefly-transmitted (Bemisia tabaci Meam 1) cytorhabdovirus infecting common bean. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 12., 2017, Piracicaba. Produtividade e sustentabilidade da cultura do feijão: do campo para a mesa: resumos. Piracicaba: CENA: IAC, 2017. p. 52. CONAFE Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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3. | | LIMA, B. P. de; ALVES-FREITAS, D. M. T.; MELO, F. L. de; CARVALHO, R. de C. P.; FARIA, J. C. de; RIBEIRO, S. G. Characterization of a new whitefly-transmitted (Bemisia tabaci Meam 1) cytorhabdovirus infecting common bean. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 12., 2017, Piracicaba. Produtividade e sustentabilidade da cultura do feijão: do campo para a mesa: resumos. Piracicaba: CENA: IAC, 2017. p. 52. CONAFE Biblioteca(s): Embrapa Arroz e Feijão. |
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4. | | FELIX, G. P.; ALVES-FREITAS, D. M. T.; LIMA, B. P. de; LACERDA, A. L. M.; CARVALHO, M. M. R. de; VIDAL, A. H.; FARIA, J. C. de; RIBEIRO, S. G. High seed transmission rates of cowpea mild mottle virus in common bean,. In: CONGRESSO BRASILEIRO DE VIROLOGIA, 29.; ENCONTRO DE VIROLOGIA DO MERCOSUL, 13., 2018, Gramado. Resumos... Brasília, DF: SBV, 2018. Poster 390. Biblioteca(s): Embrapa Arroz e Feijão. |
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5. | | FELIX, G. P.; LIMA, B. P. de; VIDAL, A. H.; ALVES-FREITAS, D. M. T.; LACERDA, A. M. L.; CARVALHO, M. M. R. de; SARAIVA, M. A. de P.; PINHEIRO, P. V.; LACORTE, C.; FARIA, J. C. de; RIBEIRO, S. da G. Cowpea mild mottle virus (CPMMV) seed transmission in common bean cultivars. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia, 2019. p. 834 Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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6. | | VIDAL, A. H.; FELIX, G. P.; LIMA, B. P. de; SANCHES, M. M.; ABREU, E. F. M.; ALVES-FREITAS, D. M. T.; LACORTE, C.; ROSA, R. C. C.; JESUS, O. N. de; CAMPOS, M. de A.; RESENDE, R. O.; VARSANI, A.; RIBEIRO, S. da G. Incidence of cucurbitaphid-borne yellowsvirus and cowpeaaphid-borne mosaic virus on Passiflora spp. plants in Bahia State, Brazil. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia, 2019., 2019 p. 833 Biblioteca(s): Embrapa Agrobiologia. |
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7. | | VIDAL, A. H.; FELIX, G. P.; LIMA, B. P. de; SANCHES, M. M.; ABREU, E. F. M.; ALVES-FREITAS, D. M. T.; LACORTE, C.; ROSA, R. C. C.; JESUS, O. N. de; CAMPOS, M. de A.; RESENDE, R. O.; VARSANI, A.; RIBEIRO, S. da G. Incidence of cucurbitaphid-borne yellowsvirus and cowpeaaphid-borne mosaic virus on Passiflora spp. plants in Bahia State, Brazil. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia, 2019. p. 833 Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 7 | |
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Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
17/12/2018 |
Data da última atualização: |
18/02/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SILVA, I. H. S. da; GOMÉZ, I.; SÁNCHEZ, J.; CASTRO, D. L. M. de; VALICENTE, F. H.; SOBERÓN, M.; POLANCZYK, R. A.; BRAVO, A. |
Afiliação: |
Igor Henrique Sena da Silva, Universidade Estadual Paulista; Isabel Goméz, Universidad Nacional Autónoma de México; Jorge Sánchez, Universidad Nacional Autónoma de México; Diana L. Martínez de Castro, Universidad Nacional Autónoma de México; FERNANDO HERCOS VALICENTE, CNPMS; Mario Soberón, Universidad Nacional Autónoma de México; Ricardo Antonio Polanczyk, Universidade Estadual Paulista; Alejandra Bravo, Universidad Nacional Autónoma de México. |
Título: |
Identification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera: Noctuidae) that bind to Cry1Ac toxin. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Plos One, San Francisco, v. 13, n. 12, e0207789, 2018. |
DOI: |
10.1371/journal.pone.0207789 |
Idioma: |
Inglês |
Conteúdo: |
Helicoverpa armigera is a polyphagous pest sensitive to Cry1Ac protein from Bacillus thuringiensis (Bt). The susceptibility of the different larval instars of H. armigera to Cry1Ac protoxin showed a significant 45-fold reduction in late instars compared to early instars. A possible hypothesis is that gut surface proteins that bind to Cry1Ac differ in both instars, although higher Cry toxin degradation in late instars could also explain the observed differences in susceptibility. Here we compared the Cry1Ac-binding proteins from second and fifth instars by pull-down assays and liquid chromatography coupled to mass spectrometry analysis (LC-MS/MS). The data show differential protein interaction patterns of Cry1Ac in the two instars analyzed. Alkaline phosphatase, and other membrane proteins, such as prohibitin and an anion selective channel protein were identified only in the second instar, suggesting that these proteins may be involved in the higher toxicity of Cry1Ac in early instars of H. armigera. Eleven Cry1Ac binindg proteins were identified exclusively in late instar larvae, like different proteases such as trypsin-like protease, azurocidin-like proteinase, and carboxypeptidase. Different aminopeptidase N isofroms were identified in both instar larvae. We compared the Cry1Ac protoxin degradation using midgut juice from late and early instars, showing that the midgut juice from late instars is more efficient to degrade Cry1Ac protoxin than that of early instars, suggesting that increased proteolytic activity on the toxin could also explain the low Cry1Ac toxicity in late instars. MenosHelicoverpa armigera is a polyphagous pest sensitive to Cry1Ac protein from Bacillus thuringiensis (Bt). The susceptibility of the different larval instars of H. armigera to Cry1Ac protoxin showed a significant 45-fold reduction in late instars compared to early instars. A possible hypothesis is that gut surface proteins that bind to Cry1Ac differ in both instars, although higher Cry toxin degradation in late instars could also explain the observed differences in susceptibility. Here we compared the Cry1Ac-binding proteins from second and fifth instars by pull-down assays and liquid chromatography coupled to mass spectrometry analysis (LC-MS/MS). The data show differential protein interaction patterns of Cry1Ac in the two instars analyzed. Alkaline phosphatase, and other membrane proteins, such as prohibitin and an anion selective channel protein were identified only in the second instar, suggesting that these proteins may be involved in the higher toxicity of Cry1Ac in early instars of H. armigera. Eleven Cry1Ac binindg proteins were identified exclusively in late instar larvae, like different proteases such as trypsin-like protease, azurocidin-like proteinase, and carboxypeptidase. Different aminopeptidase N isofroms were identified in both instar larvae. We compared the Cry1Ac protoxin degradation using midgut juice from late and early instars, showing that the midgut juice from late instars is more efficient to degrade Cry1Ac protoxin than that of early instars, suggesti... Mostrar Tudo |
Thesagro: |
Praga de Planta; Proteína; Toxina. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/188667/1/Identification-midgut.pdf
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Marc: |
LEADER 02401naa a2200253 a 4500 001 2101792 005 2019-02-18 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0207789$2DOI 100 1 $aSILVA, I. H. S. da 245 $aIdentification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera$bNoctuidae) that bind to Cry1Ac toxin.$h[electronic resource] 260 $c2018 520 $aHelicoverpa armigera is a polyphagous pest sensitive to Cry1Ac protein from Bacillus thuringiensis (Bt). The susceptibility of the different larval instars of H. armigera to Cry1Ac protoxin showed a significant 45-fold reduction in late instars compared to early instars. A possible hypothesis is that gut surface proteins that bind to Cry1Ac differ in both instars, although higher Cry toxin degradation in late instars could also explain the observed differences in susceptibility. Here we compared the Cry1Ac-binding proteins from second and fifth instars by pull-down assays and liquid chromatography coupled to mass spectrometry analysis (LC-MS/MS). The data show differential protein interaction patterns of Cry1Ac in the two instars analyzed. Alkaline phosphatase, and other membrane proteins, such as prohibitin and an anion selective channel protein were identified only in the second instar, suggesting that these proteins may be involved in the higher toxicity of Cry1Ac in early instars of H. armigera. Eleven Cry1Ac binindg proteins were identified exclusively in late instar larvae, like different proteases such as trypsin-like protease, azurocidin-like proteinase, and carboxypeptidase. Different aminopeptidase N isofroms were identified in both instar larvae. We compared the Cry1Ac protoxin degradation using midgut juice from late and early instars, showing that the midgut juice from late instars is more efficient to degrade Cry1Ac protoxin than that of early instars, suggesting that increased proteolytic activity on the toxin could also explain the low Cry1Ac toxicity in late instars. 650 $aPraga de Planta 650 $aProteína 650 $aToxina 700 1 $aGOMÉZ, I. 700 1 $aSÁNCHEZ, J. 700 1 $aCASTRO, D. L. M. de 700 1 $aVALICENTE, F. H. 700 1 $aSOBERÓN, M. 700 1 $aPOLANCZYK, R. A. 700 1 $aBRAVO, A. 773 $tPlos One, San Francisco$gv. 13, n. 12, e0207789, 2018.
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