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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
26/12/2018 |
Data da última atualização: |
09/08/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; ALFRADIQUE, V. A. P.; ARASHIRO, E. K. N.; COSENTINO, I. O.; PINTO, P. H. N.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC. |
Título: |
Dose and administration protocol for FSH used for ovarian stimulation affect gene expression in sheep cumulus-oocyte complexes. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 30, n. 9, p. 1234-1244, 2018. |
DOI: |
https://doi.org/10.1071/rd17337 |
Idioma: |
Inglês |
Conteúdo: |
The present study evaluated the effect of four ovarian stimulation protocols on the follicular population and molecular status of cumulus-oocyte complexes (COCs). Twelve Santa Inês ewes (in a cross-over design) received 80 or 120mg FSH alone in a multiple-dose (MD80 and MD120) regimen or in combination with 300IU equine chorionic gonadotrophin (eCG) in a one-shot (OS80 and OS120) protocol. The follicular population, COC recovery rate, mean COCs per ewe and the rate of brilliant Cresyl blue-positive (BCB+) COCs were similar among treatments (P>0.05). The expression of markers of oocyte competence (ZAR1, zygote arrest 1; MATER, maternal antigen that embryo requires; GDF9, growth differentiation factor 9; BMP15, bone morphogenetic protein 15; Bcl-2, B-cell lymphoma 2; BAX, Bcl-2 associated X protein) and the steroidogenic pathway (ER?, oestrogen receptor ?; LHr, LH receptor; FSHr, FSH receptor; STAR, steroidogenic acute regulatory protein) was affected by stimulation. Specifically, the expression of markers of the steroidogenic pathway was reduced with increasing FSH dose in the OS protocol. FSH at a dose of 80mg reduced the expression of FSHr and ER? in the OS versus MD protocol. Conversely, in MD protocol, only LHr was affected by increasing FSH dose. In conclusion, 80mg FSH in the MD or OS protocol was sufficient to promote the development of multiple follicles and obtain fully grown (BCB+) oocytes. The MD protocol may be more appropriate for the production of better-quality oocytes. MenosThe present study evaluated the effect of four ovarian stimulation protocols on the follicular population and molecular status of cumulus-oocyte complexes (COCs). Twelve Santa Inês ewes (in a cross-over design) received 80 or 120mg FSH alone in a multiple-dose (MD80 and MD120) regimen or in combination with 300IU equine chorionic gonadotrophin (eCG) in a one-shot (OS80 and OS120) protocol. The follicular population, COC recovery rate, mean COCs per ewe and the rate of brilliant Cresyl blue-positive (BCB+) COCs were similar among treatments (P>0.05). The expression of markers of oocyte competence (ZAR1, zygote arrest 1; MATER, maternal antigen that embryo requires; GDF9, growth differentiation factor 9; BMP15, bone morphogenetic protein 15; Bcl-2, B-cell lymphoma 2; BAX, Bcl-2 associated X protein) and the steroidogenic pathway (ER?, oestrogen receptor ?; LHr, LH receptor; FSHr, FSH receptor; STAR, steroidogenic acute regulatory protein) was affected by stimulation. Specifically, the expression of markers of the steroidogenic pathway was reduced with increasing FSH dose in the OS protocol. FSH at a dose of 80mg reduced the expression of FSHr and ER? in the OS versus MD protocol. Conversely, in MD protocol, only LHr was affected by increasing FSH dose. In conclusion, 80mg FSH in the MD or OS protocol was sufficient to promote the development of multiple follicles and obtain fully grown (BCB+) oocytes. The MD protocol may be more appropriate for the production of better-quality... Mostrar Tudo |
Palavras-Chave: |
Oócito. |
Thesagro: |
Biologia Molecular; Ovelha. |
Thesaurus Nal: |
Molecular biology; Oocytes. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02470naa a2200301 a 4500 001 2102508 005 2022-08-09 008 2018 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1071/rd17337$2DOI 100 1 $aBRAGANÇA, G. M. 245 $aDose and administration protocol for FSH used for ovarian stimulation affect gene expression in sheep cumulus-oocyte complexes.$h[electronic resource] 260 $c2018 520 $aThe present study evaluated the effect of four ovarian stimulation protocols on the follicular population and molecular status of cumulus-oocyte complexes (COCs). Twelve Santa Inês ewes (in a cross-over design) received 80 or 120mg FSH alone in a multiple-dose (MD80 and MD120) regimen or in combination with 300IU equine chorionic gonadotrophin (eCG) in a one-shot (OS80 and OS120) protocol. The follicular population, COC recovery rate, mean COCs per ewe and the rate of brilliant Cresyl blue-positive (BCB+) COCs were similar among treatments (P>0.05). The expression of markers of oocyte competence (ZAR1, zygote arrest 1; MATER, maternal antigen that embryo requires; GDF9, growth differentiation factor 9; BMP15, bone morphogenetic protein 15; Bcl-2, B-cell lymphoma 2; BAX, Bcl-2 associated X protein) and the steroidogenic pathway (ER?, oestrogen receptor ?; LHr, LH receptor; FSHr, FSH receptor; STAR, steroidogenic acute regulatory protein) was affected by stimulation. Specifically, the expression of markers of the steroidogenic pathway was reduced with increasing FSH dose in the OS protocol. FSH at a dose of 80mg reduced the expression of FSHr and ER? in the OS versus MD protocol. Conversely, in MD protocol, only LHr was affected by increasing FSH dose. In conclusion, 80mg FSH in the MD or OS protocol was sufficient to promote the development of multiple follicles and obtain fully grown (BCB+) oocytes. The MD protocol may be more appropriate for the production of better-quality oocytes. 650 $aMolecular biology 650 $aOocytes 650 $aBiologia Molecular 650 $aOvelha 653 $aOócito 700 1 $aBATISTA, R. I. T. P. 700 1 $aSOUZA-FABJAN, J. M. G. 700 1 $aALFRADIQUE, V. A. P. 700 1 $aARASHIRO, E. K. N. 700 1 $aCOSENTINO, I. O. 700 1 $aPINTO, P. H. N. 700 1 $aCAMARGO, L. S. de A. 700 1 $aFONSECA, J. F. da 700 1 $aBRANDÃO, F. Z. 773 $tReproduction, Fertility and Development$gv. 30, n. 9, p. 1234-1244, 2018.
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Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
09/08/2005 |
Data da última atualização: |
10/10/2023 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
AZEVEDO, S. S. N.; LIMA, M. A. C. de; PAES, P. de C.; SANTOS, P. de S.; SILVA, F. O. da. |
Afiliação: |
SUELLEN SORAIA NUNES AZEVED0; MARIA AUXILIADORA COELHO DE LIMA, CPATSA; PRISSILA DE CASTRO PAES; POLYANE DE SÁ SANTOS; FRANCINAIDE OLIVEIRA DA SILVA. |
Título: |
Armazenamento refrigerado da uva Superior Seedless associado a atmosfera modificada e choque de CO2. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO CIENTÍFICA, 9., 2005, Recife. Anais... Recife: FACEPE; CNPq, 2005. |
Páginas: |
p. 51-52. |
Idioma: |
Português |
Conteúdo: |
Este trabalho teve o objetivo de avaliar a eficiência do armazenamento refrigerado da uva Superior Seedless associado à atmosfera modificada e choque de CO2 |
Palavras-Chave: |
Atmosfera modificada; CO2. |
Thesagro: |
Pós-Colheita; Uva; Variedade. |
Thesaurus NAL: |
Grapes; Postharvest physiology; Postharvest technology; Postharvest treatment. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178400/1/Jornada-de-Iniciacao-Cientifica-2005-p.-51-52.pdf
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Marc: |
LEADER 00986nam a2200277 a 4500 001 1155826 005 2023-10-10 008 2005 bl uuuu u00u1 u #d 100 1 $aAZEVEDO, S. S. N. 245 $aArmazenamento refrigerado da uva Superior Seedless associado a atmosfera modificada e choque de CO2. 260 $aIn: JORNADA DE INICIAÇÃO CIENTÍFICA, 9., 2005, Recife. Anais... Recife: FACEPE; CNPq$c2005 300 $ap. 51-52. 520 $aEste trabalho teve o objetivo de avaliar a eficiência do armazenamento refrigerado da uva Superior Seedless associado à atmosfera modificada e choque de CO2 650 $aGrapes 650 $aPostharvest physiology 650 $aPostharvest technology 650 $aPostharvest treatment 650 $aPós-Colheita 650 $aUva 650 $aVariedade 653 $aAtmosfera modificada 653 $aCO2 700 1 $aLIMA, M. A. C. de 700 1 $aPAES, P. de C. 700 1 $aSANTOS, P. de S. 700 1 $aSILVA, F. O. da
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