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Registro Completo |
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Biblioteca(s): |
Embrapa Territorial. |
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Data corrente: |
02/04/2015 |
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Data da última atualização: |
01/06/2017 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
VIEIRA, S. R.; GREGO, C. R.; TOPP, G. C.; REYNOLDS, W. D. |
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Afiliação: |
SIDNEY ROSA VIEIRA, IAC; CELIA REGINA GREGO, CNPM; GEORGE CLARKE TOPP, Eastern Cereal and Oilssed Research Centre, Agriculture and Agri-Food Canada; WILLIAN DANIEL REYNOLDS, Greenhouse and Processing Crops Research Centre, Agriculture and Agri-Food Canada. |
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Título: |
Spatial Relationships Between Soil Water Content and Hydraulic Conductivity in a Highly Structured Clay Soils. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
TEIXEIRA, W. G.; CEDDIA, M. B.; OTTONI, M. V.; DONNAGEMA, G. K. (Ed.). Application of soil physics in environmental analyses: measuring, modelling and data integration. New York: Springer, 2014. |
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Páginas: |
p. 75-90. |
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Série: |
(Progress in Soil Science) |
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Idioma: |
Inglês |
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Conteúdo: |
The water, element essential for life, is present as a large reservoir in the soil. Soil water content varies in space as a consequence of the variability of other related properties and its study is necessary to know how this variation occurs in space and time. The objective of this study was to assess the spatial and temporal variability of soil water content and its relationships with hydraulic conductivity. Soil water content was measured with a TDR equipment and the hydraulic conductivity was measured with a constant head well permeameter at 15 and 50 em depth, in a nearly flat 1.2 ha field at the Central Experimental Farm of the Agriculture Canada, Ottawa. The soil is classified as a Rideau soil series, is primarily clay to silty clay texture. Sampling was made on a 10 m spacing square grid with 164 sampling points. Soil water content was measured on 33 dates during the frost free months in 1987, 1988 and 1989. The rate of change in soil water content was calculated for the periods which did not have any rain. The spatial variability was analyzed examining the descriptive statistical parameters, the parameters of the models fitted to individual semivariograms as a function of time and the maps obtained by kriging interpolation. It was concluded that soil structure played a very important role on the spatial distribution of hydraulic conductivity and water content. Temporal stability of water in the soil is quite different during the year probably because of the hydraulic conductivity role. Parabolic trend removal MenosThe water, element essential for life, is present as a large reservoir in the soil. Soil water content varies in space as a consequence of the variability of other related properties and its study is necessary to know how this variation occurs in space and time. The objective of this study was to assess the spatial and temporal variability of soil water content and its relationships with hydraulic conductivity. Soil water content was measured with a TDR equipment and the hydraulic conductivity was measured with a constant head well permeameter at 15 and 50 em depth, in a nearly flat 1.2 ha field at the Central Experimental Farm of the Agriculture Canada, Ottawa. The soil is classified as a Rideau soil series, is primarily clay to silty clay texture. Sampling was made on a 10 m spacing square grid with 164 sampling points. Soil water content was measured on 33 dates during the frost free months in 1987, 1988 and 1989. The rate of change in soil water content was calculated for the periods which did not have any rain. The spatial variability was analyzed examining the descriptive statistical parameters, the parameters of the models fitted to individual semivariograms as a function of time and the maps obtained by kriging interpolation. It was concluded that soil structure played a very important role on the spatial distribution of hydraulic conductivity and water content. Temporal stability of water in the soil is quite different during the year probably because of the hydraul... Mostrar Tudo |
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Palavras-Chave: |
Semivariogram; TDR temporal stability. |
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Thesaurus Nal: |
Geostatistics. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/121771/1/4427.pdf
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Marc: |
LEADER 02329naa a2200217 a 4500
001 2012680
005 2017-06-01
008 2014 bl uuuu u00u1 u #d
100 1 $aVIEIRA, S. R.
245 $aSpatial Relationships Between Soil Water Content and Hydraulic Conductivity in a Highly Structured Clay Soils.
260 $c2014
300 $ap. 75-90.
490 $a(Progress in Soil Science)
520 $aThe water, element essential for life, is present as a large reservoir in the soil. Soil water content varies in space as a consequence of the variability of other related properties and its study is necessary to know how this variation occurs in space and time. The objective of this study was to assess the spatial and temporal variability of soil water content and its relationships with hydraulic conductivity. Soil water content was measured with a TDR equipment and the hydraulic conductivity was measured with a constant head well permeameter at 15 and 50 em depth, in a nearly flat 1.2 ha field at the Central Experimental Farm of the Agriculture Canada, Ottawa. The soil is classified as a Rideau soil series, is primarily clay to silty clay texture. Sampling was made on a 10 m spacing square grid with 164 sampling points. Soil water content was measured on 33 dates during the frost free months in 1987, 1988 and 1989. The rate of change in soil water content was calculated for the periods which did not have any rain. The spatial variability was analyzed examining the descriptive statistical parameters, the parameters of the models fitted to individual semivariograms as a function of time and the maps obtained by kriging interpolation. It was concluded that soil structure played a very important role on the spatial distribution of hydraulic conductivity and water content. Temporal stability of water in the soil is quite different during the year probably because of the hydraulic conductivity role. Parabolic trend removal
650 $aGeostatistics
653 $aSemivariogram
653 $aTDR temporal stability
700 1 $aGREGO, C. R.
700 1 $aTOPP, G. C.
700 1 $aREYNOLDS, W. D.
773 $tTEIXEIRA, W. G.; CEDDIA, M. B.; OTTONI, M. V.; DONNAGEMA, G. K. (Ed.). Application of soil physics in environmental analyses: measuring, modelling and data integration. New York: Springer, 2014.
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Registro original: |
Embrapa Territorial (CNPM) |
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
03/03/2017 |
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Data da última atualização: |
08/03/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SANTOS, R. C. dos; SILVA, C. R. C. da; SOARES, T. da C; CAVALCANTI, J. J. V.; LIMA, L. M. de; MARTINS, E. S.; PONTES, R. G. M. S. de. |
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Afiliação: |
ROSEANE CAVALCANTI DOS SANTOS, CNPA; CARLIANE REBECA COELHO da SILVA, UFRPE; TAÍZA da CUNHA SOARES, UFRPE; JOSE JAIME VASCONCELOS CAVALCANTI, CNPA; LIZIANE MARIA DE LIMA, CNPA; ÉRICA SOARES MARTINS, Cenargen; ROSE GOMES MONNERAT SOLON DE PONTES, Cenargen. |
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Título: |
Relative expression of CRy10 in GM cotton lines resistant to boll weevil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 87. |
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Idioma: |
Inglês |
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Conteúdo: |
Boll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and high in 8H- 336 (14x). These data agreed with previous results obtained by ELISA assays. Eleven lines derivate from 8H-336 (T1) were analyzed by PCR assays with genomic DNA, using 2 primer combinations. More than 50% showed amplicons confirming the presence of gene in selected lines. Taking in account that a reasonable level of resistance should overcoming 2 ug of protein/g tissue, we suggested that 8H-366 is the best genotype for control the cotton boll weevil. This material will be further advanced for entomological assays with larvae and adults of boll weevil. MenosBoll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and ... Mostrar Tudo |
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Palavras-Chave: |
Boll weevil. |
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Thesagro: |
Algodão; Anthonomus Grandis; Bicudo. |
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Thesaurus NAL: |
Cotton. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157044/1/Relative-expression-of-Cry10.pdf
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Marc: |
LEADER 02851nam a2200241 a 4500
001 2066096
005 2017-03-08
008 2016 bl uuuu u00u1 u #d
100 1 $aSANTOS, R. C. dos
245 $aRelative expression of CRy10 in GM cotton lines resistant to boll weevil.$h[electronic resource]
260 $aIn: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 87.$c2016
520 $aBoll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and high in 8H- 336 (14x). These data agreed with previous results obtained by ELISA assays. Eleven lines derivate from 8H-336 (T1) were analyzed by PCR assays with genomic DNA, using 2 primer combinations. More than 50% showed amplicons confirming the presence of gene in selected lines. Taking in account that a reasonable level of resistance should overcoming 2 ug of protein/g tissue, we suggested that 8H-366 is the best genotype for control the cotton boll weevil. This material will be further advanced for entomological assays with larvae and adults of boll weevil.
650 $aCotton
650 $aAlgodão
650 $aAnthonomus Grandis
650 $aBicudo
653 $aBoll weevil
700 1 $aSILVA, C. R. C. da
700 1 $aSOARES, T. da C
700 1 $aCAVALCANTI, J. J. V.
700 1 $aLIMA, L. M. de
700 1 $aMARTINS, E. S.
700 1 $aPONTES, R. G. M. S. de
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